| تعداد نشریات | 24 |
| تعداد شمارهها | 916 |
| تعداد مقالات | 8,202 |
| تعداد مشاهده مقاله | 15,918,399 |
| تعداد دریافت فایل اصل مقاله | 13,468,759 |
Orexin A promotes granulosa cell secretion of progesterone in sheep | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 9، دوره 20، شماره 2 - شماره پیاپی 67، 2019، صفحه 136-142 اصل مقاله (437.38 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2019.5264 | ||
| نویسندگان | ||
| M. Li1؛ N. Zu1؛ C. S. Zhang1؛ M. Y. Xie1؛ Y. Z. Liu2؛ X. J. Xu* 3 | ||
| 1MSc Student in Developmental Biology, Animal Biotechnology Laboratory, College of Veterinary Medicine, The Inner Mongolia Agricultural University, Hohehot, Inner Mongolia, China | ||
| 2Agriculture and Animal Husbandry Department of Inner Mongolia Autonomous Region, Hohehot, Inner Mongolia, China | ||
| 3Animal Biotechnology Laboratory, College of Veterinary Medicine, The Inner Mongolia Agricultural University, Hohehot, Inner Mongolia, China | ||
| چکیده | ||
| Background: Orexin A, a small-molecule peptide, can regulate female hormones, but limited evidence for its mechanism of activity exists in ovine. Aims: The objective of this study was to investigate the effect of orexin A on progesterone (P4) secretion in cultured granulosa of sheep follicles. Methods: Sheep ovarian granulosa were isolated and identified, pre-incubated with luteinizing hormone (LH) (2.5 IU/ml), follicle-stimulating hormone (FSH) (2.5 IU/ml), or oestrogen (1 µg/ml); and cultured in vitro. The pretreated sheep ovarian granulosa were subsequently cultured with different concentrations (1 nM, 10 nM, 58 nM, 100 nM, and 145 nM) of orexin A for varying amounts of time (0 h, 24 h, 48 h, and 72 h). Then, the expression levels of P4, steroidogenic acute regulatory protein (StAR), 3β-Hydroxysteroid dehydrogenase (3β-HSD) and cytochrome P450 (CYP11) were determined. Results: The results showed that the sheep ovarian granulosa were correctly identified. The different concentrations of orexin A promoted the secretion of P4 from granulosa in the ovine ovary compared with that in the control. The expression of StAR, 3β-HSD and P450 (CYP11) gradually increased, and then decreased with increasing concentrations of orexin A, but the expression of P450 (CYP11) decreased with the increase of time. Conclusion: These results revealed that orexin A promotes the secretion of P4 by regulating the expression of StAR, 3β-HSD, and P450 (CYP11). Understanding the mechanism underlying the promotion of P4 by orexin A could open new therapeutic possibilities in the treatment of hormone homeostasis. | ||
| کلیدواژهها | ||
| 3β-HSD؛ P450 (CYP11)؛ progesterone؛ Orexin A؛ StAR | ||
| مراجع | ||
|
Aston-Jones, G; Smith, RJ; Sartor, GC; Moorman, DE; Massi, L; Tahsili-Fahadan, P and Richardson, KA (2010). Lateral hypothalamic orexin/hypocretin neurons: a role in reward-seeking and addiction. Brain Res. J. Virol., 1314: 74-90.
Cai, XJ; Widdowson, PS; Harrold, J; Wilson, S; Buckingham, RE; Arch, JR; Tadayyon, M; Clapham, JC; Wilding, J and Williams, G (1999). Hypothalamic orexin expression: modulation by blood glucose and feeding. Diabetes J. Virol., 48: 2132-2137. Cataldi, NI; Lux-Lantos, VA and Libertun, C (2010). Effects of orexins A and B on expression of orexin receptors and progesterone release in luteal and granulosa ovarian cells. Regul. Pept., 178: 56-63.
Cravioto, MD; Ulloa-Aguirre, A; Bermudez, JA; Herrera, J; Lisker, R; Mendez, JP and Perez-Palacios, G (1986). A new inherited variant of the 3 beta-hydroxysteroid dehydrogenase-isomerase deficiency syndrome: evidence for the existence of two isoenzymes. J. Clin. Endocrinol. Metab., 63: 360-367. Diep, CH; Daniel, AR; Mauro, LJ; Knutson, TP and Lange, CA (2015). Progesterone action in breast, uterine, and ovarian cancers. J. Mol. Endocrinol., 54: R31-53. Gaskins, GT and Moenter, SM (2012). Orexin a suppresses gonadotropin-releasing hormone (GnRH) neuron activity in the mouse. Endocrinology. 153: 3850-3860. Hosseini, A; Niasari-Naslaji, A; Vojgani, M and Gharagozloo, F (2018). Effect of time of eCG administration on the fate of ovarian follicle in Holstein heifers. Iran. J. Vet. Res., 19(1): 15-21. Hu, MC; Hsu, HJ; Guo, IC and Chung, BC (2004). Function of Cyp11a1 in animal models. Mol. Cell. Endocrinol., 215: 95-100. Ida, T; Nakahara, K; Murakami, T; Hanada, R; Nakazato, M and Murakami, N (2000). Possible involvement of orexin in the stress reaction in rats. Biochem. Biophys. Res. Commun., 270: 318-323. Kaynard, AH; Periman, LM; Simard, J and Melner, MH (1992). Ovarian 3 beta-hydroxysteroid dehydrogenase and sulfated glycoprotein-2 gene expression are differentially regulated by the induction of ovulation, pseudopregnancy, and luteolysis in the immature rat. Endocrinology. 130: 2192-2200. Kok, SW; Roelfsema, F; Overeem, S; Lammers, GJ; Frolich, M; Meinders, AE and Pijl, H (2004). Pulsatile LH release is diminished, whereas FSH secretion is normal, in hypocretin-deficient narcoleptic men. Am. J. Physiol. Endocrinol. Metab., 287: E630-6. Komatsu, K and Masubuchi, S (2017). The concentration-dependent effect of progesterone on follicle growth in the mouse ovary. J. Reprod. Dev., 63: 271-277. Lachance, Y; Luu-The, V; Labrie, C; Simard, J; Dumont, M; de Launoit, Y; Guerin, S; Leblanc, G and Labrie, F (1992). Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells. J. Biol. Chem., 267: 3551. Li, J; Hu, Z and de Lecea, L (2014). The hypocretins/orexins: integrators of multiple physiological functions. Br. J. Pharmacol. J. Virol., 171: 332-350. Miller, WL and Strauss, JF (1999). Molecular pathology and mechanism of action of the steroidogenic acute regulatory protein, StAR. J. Steroid. Biochem. Mol. Biol., 69: 131-141. Muschamp, JW; Dominguez, JM; Sato, SM; Shen, RY and Hull, EM (2007). A role for hypocretin (orexin) in male sexual behavior. J. Neurosci., 27: 2837-2845. Piper, DC; Upton, N; Smith, MI and Hunter, AJ (2000). The novel brain neuropeptide, orexin-A, modulates the sleep-wake cycle of rats. Eur. J. Neurosci., 12: 726-730. Pu, S; Jain, MR; Kalra, PS and Kalra, SP (1998). Orexins, a novel family of hypothalamic neuropeptides, modulate Puett, D; Angelova, K; da Costa, MR; Warrenfeltz, SW and Fanelli, F (2010). The luteinizing hormone receptor: insights into structure-function relationships and hormone-receptor-mediated changes in gene expression in ovarian cancer cells. Mol. Cell. Endocrinol., 329: 47-55. Russell, SH; Small, CJ; Kennedy, AR; Stanley, SA; Seth, A; Murphy, KG; Taheri, S; Ghatei, MA and Bloom, SR (2001). Orexin A interactions in the hypothalamo-pituitary gonadal axis. Endocrinology. 142: 5294-5302. Smart, D; Jerman, JC; Brough, SJ; Rushton, SL; Murdock, PR; Jewitt, F; Elshourbagy, NA; Ellis, CE; Middlemiss, DN and Brown, F (1999). Characterization of recombinant human orexin receptor pharmacology in a Chinese hamster ovary cell-line using FLIPR. Br. J. Pharmacol., 128: 1-3. Stocco, DM (2000). The role of the StAR protein in steroidogenesis: challenges for the future. J. Endocrinol. J. Virol., 164: 247-253. Stocco, DM (2001). StAR protein and the regulation of steroid hormone biosynthesis. Annu. Rev. Physiol. J. Virol., 63: 193-213. Storbeck, KH; Swart, P and Swart, AC (2007). Cytochrome P450 side-chain cleavage: insights gained from homology modeling. Mol. Cell. Endocrinol., 265-266: 65-70. Wei, S; Gong, Z; Guo, H; Zhang, T and Ma, Z (2017). FSH and eCG impact follicles development and expression of ovarian FSHR and caspase-9 in mice. Iran. J. Vet. Res., 18: 79-85. | ||
|
آمار تعداد مشاهده مقاله: 1,051 تعداد دریافت فایل اصل مقاله: 571 |
||