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Gill lesions and mortality in common carp (Cyprinus carpio) with a dense bloom of Heterosigma-like algae in Khuzestan province, Iran | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 12، دوره 20، شماره 1 - شماره پیاپی 66، 2019، صفحه 64-67 اصل مقاله (323.13 K) | ||
| نوع مقاله: Scientific report | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2019.5146 | ||
| نویسندگان | ||
| R. Peyghan* 1؛ A. Rezaei2؛ Z. Tulaby Dezfuly3؛ M. Halimi3 | ||
| 1Departmentof Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 3Ph.D. Student in Aquatic Animal Health, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| چکیده | ||
| Background: Widespread common carp (Cyprinus carpio) mortalities have been recorded in Khuzestan province fish farms in recent years. In summer of 2017 two cases of harmful algal bloom were encountered that led to massive mortality in common carp in Khuzestan, Iran. Aims: The aim of this study was to identify the possible etiologic agent of two mortalities with characteristic symptoms of gill lesions due to harmful algae. Methods: Water samples were collected and 5 moribund fishes were examined by histophatologic, scanning electron microscopic and PCR examination. Results: In wet smear preparations, a lot of algal cells and fragments, and sloughed, necrotic epithelial cells were observed between the lamellae. In histopathologic examination of gills, hyperplasia, necrosis and algal cells surrounded by hyperplastic cells were seen in tissue sections. No inflammatory cell aggregation was noticed. In scanning electron microscopic examination the algae was found attached to the gill surface (cell diameter: 8.5 ± 4.2 µm) with 2 equal flagella. Conclusion: Phytoplankton analysis using direct microscopy and electron microscopy, morphologically resembling Heterosigma was identified, however, in PCR tests, Heterosigma analysis showed negative results, therefore the causative agent was called “Heterosigma-like” algae. | ||
| کلیدواژهها | ||
| Common carp؛ Gill lesions؛ Heterosigma sp؛ Khuzestan province؛ Mortality | ||
| مراجع | ||
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Abou El-Gheit, EN; Abdo, MH and Mahmoud, SA (2012). Impacts of blooming phenomenon on water quality and fishes in Qarunlake, Egypt. J. Environ. Sci. Eng., 3: 11-23.
Akase, SI; Yoshikawa, T; Hayakawa, N; Maeda, H and Sakata, T (2004). Molecular identification of red tide-causing microalga Heterosigma akashiwo strains based on their chloroplast DNA sequences. Fish. Sci., 70: 1043-1050.
Fredrickson, KA; Storm, SL; Crim, R and Coyne, K (2011). Interstrain variability in physiology and genetics of Heterosigma akashiwo (Raphidophyceae) from the west coast of North America. J. Phycol., 47: 25-35.
Gilad, O; Yun, S; Andree, KB; Adkison, MA; Zlotkin, A; Bercovier, H; Eldar, A and Hedrick RP (2002). Initial characteristics of koi herpesvirus and development of a polymerase chain reaction assay to detect the virus in koi, Cyprinus carpio koi. Dis. Aquat. Organ., 48: 101-108.
Hara, Y; Inouye, I and Chihara, M (1985). Morphology, ultrastructure of Olisthodiscus luteus (Raphidophyceae) with special reference to the taxonomy. Bot. Mag., 98: 251-262.
Higashi, A; Nagai, S; Seone, S and Ueki, S (2017). A hypervariable mitochondrial protein coding sequence associated with geographical origin in a cosmopolitan bloom-forming alga, Heterosigma akashiwo. Biol. Lett., 13: 20160976.
Martinez, R; Orive, E; Laza-Martinez, A and Seoane, S (2010). Growth response of six strains of Heterosigma akashiwo to varying temperature, salinity and irradiance conditions. J. Plankton. Res., 32: 529-538.
Shikata, T; Nagasoe, S; Matsubara, T; Yoshikawa, S; Yamasaki, Y; Shimasaki, Y; Oshima, Y; Jenkinson, IR and Honjo, T (2008). Factors influencing the initiation of blooms of the raphidophyte Heterosigma akashiwo and the diatom Skeletonema costatum in a port in Japan. Limnol. Oceanogr., 53: 2503-2518.
Siwicki, AK; Lepa, A; Malaczewska, J; Kazuñ, B; Kazun, K and Terech-Majewska, E (2006). Isolation and identification of carp interstitial nephritis and gill necrosis virus (CNGV) in fingerling common carp (Cyprinus carpio L.). Arch. Pol. Fish., 14: 157-167.
Storm, SL; Harvey, EL; Fredrickson, KA and Menden-Deuer, S (2013). Broad salinity tolerance as a refuge from predation in the harmful raphidophyte alga Heterosigma akashiwo (Raphidophyceae). J. Phycol., 49: 20-31.
Taylor, FJR and Haigh, R (1993). The ecology of fish-killing blooms of the chloromonad flagellate Heterosigma in the Strait of Georgia and adjacent waters. In: Smayda, TJ and Shimizu, Y (Eds.), Toxic phytoplankton blooms in the sea. Proceedings of the Fifth International Conference on Toxic Marine Phytoplankton, Newport, Rhode Island, USA. 28 Oct-1 Nov 1991. PP: 705-710.
Yu, J; Yang, G and Tian, J (2010). The effects of the harmful algae Heterosigma akashiwo on cultures of Schmackeria inopinus (Copepoda, Calanoida). J. Sea. Res., 64: 287-294. | ||
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