| تعداد نشریات | 24 |
| تعداد شمارهها | 851 |
| تعداد مقالات | 7,575 |
| تعداد مشاهده مقاله | 13,413,646 |
| تعداد دریافت فایل اصل مقاله | 11,580,412 |
Cytotoxic effects of three Persian Gulf species of Holothurians | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 4، دوره 20، شماره 1 - شماره پیاپی 66، 2019، صفحه 19-26 اصل مقاله (449.16 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2019.5138 | ||
| نویسندگان | ||
| S. Mashjoor؛ M. Yousefzadi* | ||
| Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran | ||
| چکیده | ||
| Background: Pharmaceutical industries around the world are struggling for finding new approaches to fight cancer and many researchers are involved in this process to find new drug candidates. Aims: The current study aimed at investigating the new marine natural products with anticancer potential from the three Persian Gulf Holothuria sea cucumbers. Methods: We evaluated the cytotox activity of different organs of three Holothuria sea cucumbers species (H. scabra, H. parva, and H. leucospilota) using organic extract (OE): n-Hexane (nH), ethyl acetate (E), and methanol (M). Cytotoxicity potential of three fractions was estimated using two toxicity models: brine shrimp (Artemia salina) lethality assay (BSA) and tetrazolium-based colorimetric assay (MTT) assay in human cancer cell lines (MCF-7( and normal cell lines (HeLa). Results: The data illustrated that toxicity depends on concentration but BSA was highest for the M extracts of cuvierian tubules (CT) organs of H. leucospilota (up to 95% at 1000 µg/ml, LC50 = 616.4 μg/ml) and respiratory tree (RT) organs of H. parva (up to 86% at 1000 µg/ml, LC50 = 607.2 μg/ml). Based on cell lines, the more effective extracts were noticed for E fractions of CT organs of H. leucospilota (up to 85% at 250 µg/ml, LC50 = 37.25 μg/ml) against MCF-7 and for E extracts of intestine tract (IT) organs of H. parva (up to 80% at 250 µg/ml, LC50 = 46.25 μg/ml) against HeLa cells. This variation indicates that the possible cytotoxic compounds in fractions are selective toxicity toward cell lines. Conclusion: The data demonstrated that Holothuria species are an interesting source for discovery of drugs. | ||
| کلیدواژهها | ||
| Biological activity؛ Cell line؛ Persian Gulf؛ Sea cucumber؛ Secondary metabolites | ||
| مراجع | ||
|
Afkhami, M; Ehsanpour, M; Khazaali, A; Kamrani, E; Mokhlesi, A and Darvish, BK (2012). Sea cucumber fisheries of Qeshm Island, Persian Gulf. SPC. Beche-de-mer. Info. Bull., 32: 60-61.
Albuntana, A; Yasman, Y and Wardhana, W (2011). Toxicity test of extracts of the four sea cucumber (family Holothuriidae) from East Penjaliran Island, Seribu Islands, Jakarta based on the brine shrimp lethality test (Bslt). J. Ilmu. Teknol. Kel. Trop., 3: 65-72.
Althunibat, OY; Ridzwan, BH; Taher, M; Daud, JM; Ikeda, MA and Zali, BI (2009). In vitro antioxidant and ntiproliferative activities of three Malaysian sea cucumber species. Eur. J. Sci. Res., 37: 376-387.
Alves, R; Preto, M; Vasconcelos, V; Oliveira, RS and Martins, R (2015). Cytotoxicity induced by extracts of Pisolithus tinctorius spores on human cancer and normal cell lines-evaluation of the anticancer potential. J. Toxicol. Environ. Health A. 78: 840-847.
Aminin, DL; Menchinskaya, ES; Pisliagin, EA; Silchenko, AS; Avilov, SA and Kalinin, VI (2015). Anticancer activity of sea cucumber triterpene glycosides. Mar. Drugs. 13: 1202-1223.
Assawasuparerk, K; Rawangchue, T and Phonarknguen, R (2016). Scabraside D derived from sea cucumber induces apoptosis and inhibits metastasis via iNOS and STAT-3 expression in human cholangiocarcinoma xenografts. Asian Pac. J. Cancer Prev., 17: 2151-2157.
AVMA (American Veterinary Medical Association) (2013). AVMA Guidelines for the Euthanasia of Animals, 2013. Ed https://www.avma.org/KB/Policies/Documents/euthanasia.pdf.
Bordbar, S; Anwar, F and Saari, N (2011). High-value components and bioactives from sea cucumbers for functional foods-A Review. Mar. Drugs. 9: 1761-1805.
Cancer Genome Atlas Network (2012). Comprehensive molecular portraits of human breast tumours. Nature. 490: 61-70.
Caulier, G; Flammang, P; Rakotorisoa, P; Gerbaux, P; Demeyer, M and Eeckhaut, I (2013). Preservation of the bioactive saponins of Holothuria scabra through the processing of trepang. Car. Biol. Mar., 54: 685-690.
Dabbagh, AR (2011). A sea cucumber, Holothuria (Selenkothuria) parva Krauss in Lampert, 1885 for the first time in the Bandar-E. Lengeh coast (Persian Gulf, Iran). World J. Zool., 6: 243-245.
Ehsanpour, Z; Archangi, B; Salimi, M; Salari, MA and Zolgharnein, H (2015). Cytotoxic assessment of extracted fractions of sea cucumber Holothuria parva on cancer cell line (MCF7) and normal cells. joc., 6: 89-96 (in Persian).
Fitch, K; Iwasaki, K and Pyenson, B (2010). Parity for oral and intravenous injected cancer drugs. Milliman Report.
Han, H; Li, L; Yi, YH; Wang, XH and Pan, MX (2012a). Triterpene glycosides from sea cucumber Holothuria scabra with cytotoxic activity. Chin. J. Herb Med., 4: 183-188.
Han, H; Yi, Y; Xu, Q; La, M and Zhang, H (2009). Two new cytotoxic triterpene glycosides from the sea cucumber Holothuria scabra. Planta Med., 75: 1608-1612.
Han, H; Yi, Y; Zhang, W; Wang, X; Pan, M and Li, L (2012b). Cytotoxic triterpene glycosides from sea cucumber Holothuria leucospilota. Chin. Pharmaceut. J., 47: 346-350.
Han, H; Zhang, W; Yi, Y; Liu, B; Pan, M and Wang, X (2010). A novel sulfated holostane glycoside from sea cucumber Holothuria leucospilota. Chem. Biodivers. 7: 1764-1769.
Hickman, CJ (1998). A fieldguide to sea stars and other echinoderms of Galapagos. 1st Edn., Lexington, VA, USA, Sugar Spring Press. PP: 1-83.
Janakiram, N; Mohammed, A and Rao, C (2015). Sea cucumbers metabolites as potent anti-cancer agents. Mar. Drugs. 13: 2909-2923.
Kerr, AM and Kim, J (2001). Phylogeny of Holothuroidea (Echinodermata) inferred from morphology. Zool. J. Linn Soc., 133: 63-81.
Layson, R; Criselda, M; Rodil, A; Mojica, E and Deocaris, C (2014). Potential anti-cancer and anti-bacterial activities of Philippine Echinoderm extracts. Trop. Life Sci. Res., 4: 175-181.
Mashjoor, S and Yousefzadi, M (2017). Holothurians antifungal and antibacterial activity to human pathogens in the Persian Gulf. J. Mycol. Méd., 27: 46-56.
Mazzaferro, S; Bouchemal, K and Ponchel, G (2013). Oral delivery of anticancer drugs I: general considerations. Drug Discov. Today. 18: 25-34.
Meyer, BN; Ferrigni, NR; Putnam, JE; Jacobsen, LB; Nichols, DE and McLaughlin, JL (1982). Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med., 45: 31-34.
Ming, JS; Guan, JH; Chieh, HW; Jwo, SC; Heng, YH; Shu, J and Jing, GC (2008). Ethanol extract of Dunaliella salina induces cell cycle arreat and apoptosis in A549 Human Non-small cell lung cancer. In vivo. 22: 369-378.
Mohammadizadeh, F; Ehsanpor, M; Afkhami, M; Mokhlesi, A; Khazaali, A and Montazeri, S (2013a). Evaluation of antibacterial, antifungal and cytotoxic effects of Holothuria scabra from the North coast of the Persian Gulf. J. Mycol. Méd., 23: 225-229. Mohammadizadeh, F; Ehsanpor, M; Afkhami, M; Mokhlesi, A; Khazaali, A and Montazeri, S (2013b). Antibacterial, antifungal and cytotoxic effects of a sea cucumber Holothuria leucospilota, from the North coast of the Persian Gulf. J. Mar. Biol. Assoc. UK. 93: 1401-1405.
Mokhlesi, A; Saeidnia, S; Gohari, AR; Shahverdi, AR; Nasrolahi, A; Farahani, F; Khoshnood, R and Eshaghi, N (2012). Biological activities of the sea cucumber Holothuria leucospilota. Asian J. Anim. Veterin. Advanc., 7: 243-249.
Nguyen, HD; Van Thanh, N; Van Kiem, P; Le, MH; Chau, VM and Young, HK (2007). Two new triterpene glycosides from the Vietnamese sea cucumber Holothuria scabra. Arch. Pharm. Res., 30: 1387-1391.
Ridzwan, B; Kaswandi, M; Azman, Y and Fuad, M (1995). Screening for antibacterial agents in three species of sea cucumber from coastal areas of Sabah. Gene Pharmacol., 26: 1539-1543.
Roginsky, A; Singh, B; Ding, XZ; Collin, P; Woodward, C; Talamonti, MS; Bell, RH and Adrian, TE (2004). Frondanol (R)-A5p from the sea cucumber, Cucumaria frondosa induces cell cycle arrest and apoptosis in pancreatic cancer cells. Pancreas. 29: 335.
Samyn, Y; Vanden Spiegel, D and Massin, C (2006). Taxonomy of sea cucumbers in Comores. ABC Taxa., 1: 1-130.
Sarhadizadeh, N; Afkhami, M and Ehsanpour, M (2014). Evaluation bioactivity of a sea cucumber, Stichopus hermanni from Persian Gulf. Eur. J. Exp. Biol., 4: 254-258.
Seydi, E; Motallebi, A; Dastbaz, M; Dehghan, S; Salimi, A; Nazemi, M and Pourahmad, J (2015). Selective toxicity of Persian Gulf sea cucumber (Holothuria parva) and sponge (Haliclona oculata) methanolic extracts on liver mitochondria isolated from an animal model of hepatocellular carcinoma. Hepat Mon., 15: e33073.
Shokrgozar, MA; Zali, H; Rezaie, TM and Aman Zadeh, A (2007). A comparing two MTT calorimetry and triban blue methods for determining citotoxicity Cal Protectin on human stomach carcinoma cells in vitro. Kousar Med. J., 12: 127-137.
Soltani, M and Baharara, J (2014). Antioxidant and antiprolifereative capacity of dichloromethane extract of Holoturia leucospilota sea cucumber. Int. J. Cel. Mol. Bio., 2014: 1-9.
Suarez-Jimenez, GM; Burgos-Hernandez, A and Ezquerra-Brauer, JM (2012). Bioactive peptides and depsipeptides with anti-cancer potential-sources from marine animals. Mar. drugs. 10: 963-986.
Taiyeb-Ali, TB; Zainuddin, SLA; Swaminathan, D and Yaacob, H (2003). Efficacy of “Gamadent” toothpaste on the healing of gingival tissues: a preliminary report. J. Oral Sci., 45: 153-159.
Tian, F; Zhang, X; Tong, Y; Yi, Y; Zhang, S; Li, L; Sun, P; Lin, L and Ding, JPE (2005). A new sulfated saponin from sea cucumber, exhibits anti-angiogenic and anti-tumor activities in vitro and in vivo. Cancer Biol. Therap., 4: 874-882.
Vargo-Gogola, T and Rosen, JM (2007). Modelling breast cancer: one size does not fit all. Nature Rev. Cancer. 7: 659-672.
Wang, J; Han, H; Chen, X; Yi, Y and Sun, H (2014). Cytotoxic and apoptosis-inducing activity of triterpene glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 cells. Mar. drugs. 12: 4274-4290. | ||
|
آمار تعداد مشاهده مقاله: 1,037 تعداد دریافت فایل اصل مقاله: 912 |
||