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Quantification and comparison of TLR2 activity in monocyte-derived macrophages of zebu and crossbred cattle | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 6، دوره 19، شماره 4 - شماره پیاپی 65، 2018، صفحه 283-289 اصل مقاله (253.63 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2018.5057 | ||
| نویسندگان | ||
| A. Baranwal؛ A. Sonwane* ؛ A. Chauhan؛ M. Panigrahi؛ A. K. Sharma | ||
| Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, Uttar Pradesh, India | ||
| چکیده | ||
| The present study was conducted to quantify and compare TLR2 (toll-like receptor 2) activity in monocyte-derived macrophages of zebu (Tharparkar) and crossbred (Holstein-Friesian × Jersey × Brown Swiss × Hariana) cattle. The cells were either induced with Pam3CSK4 or kept as control. The TLR2 activity was quantified in terms of IκB-α inhibitory subunit (NFKBIA) messenger RNA (mRNA) copies using real-time, one-step reverse transcription-polymerase chain reaction (RT-PCR). Toll-like receptor 2 activity of induced cells was in the range of 1060421 ± 477937 (n=3) to 3514715 ± 290222 (n=3) copies for Tharparkar cattle (n=7) and in the range of 1365532 ± 47243 (n=3) to 3016510 ± 172340 (n=3) copies for the crossbred cattle (n=7). For uninduced cells, this activity was within the range of 117 ± 51 (n=3) to 293 ± 103 (n=3) copies for the Thraparkar cattle (n=7), and in the range of 182 ± 122 (n=3) to 296 ± 88 (n=3) copies for the crossbred cattle (n=7). The TLR2 activity of induced cells in both groups was found to be significantly higher than that of the respective uninduced cells (P<0.0001). Furthermore, upon comparison, TLR2 activities of induced and uninduced cells of the Tharparkar were not found to be significantly different from those of the crossbred cattle (P=0.8154 and P=0.6670). In the present study, we have quantified and compared, for the first time, TLR2 activity in terms of NFKBIA mRNA copies in monocyte-derived macrophages of Tharparkar and crossbred cattle and found that both have equivalent TLR2 activity. | ||
| کلیدواژهها | ||
| Crossbred؛ Monocyte-derived macrophages؛ NFKBIA؛ Tharparkar؛ TLR2 | ||
| مراجع | ||
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Adams, LG and Schutta, JC (2010). Natural resistance against brucellosis: a review. Open. Vet. Sci. J., 4: 61-71.
Adams, LG and Templeton, JW (1998). Genetic resistance to bacterial diseases of animals. Rev. Sci. Tech. Off. Int. Epiz., 17: 200-219.
Aliprantis, AO; Weiss, DS and Zychlinsky, AJ (2001). Toll-like receptor-2 transduces signals for NF-kappa B activation, apoptosis and reactive oxygen species production. Endotoxin Res., 7: 287-291.
Aliprantis, AO; Yang, RB; Mark, MR; Suggett, S; Devaux, B; Radolf, JD; Klimpel, GR; Godowski, P and Zychlinsky, A (1999). Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2. Science. 285: 736-739.
Barthel, R; Feng, J; Piedrahita, JA; Mcmurray, JA; Templeton, DN and Adams, LG (2001). Stable transfection of bovine Nramp1 gene into murine RAW264.7 cells: effect on Brucella abortus survival. Infect. Immun., 69: 3110-3119.
Basith, S; Manavalan, B; Lee, G; Kim, SG and Choi, S (2011). Toll-like receptor modulators: a patent review (2006-2010). Expert Opin. Ther. Pat., 21: 927-944.
Beutler, B (2004). Innate immunity: an overview. Mol. Immunol., 40: 845-859.
Blackwell, JM and Searle, S (1999). Genetic regulation of macrophage activation: understanding the function of Nramp1 (=Ity/Lsh/Bcg). Immunol. Lett., 65: 73-80.
Bock, RE; Kingston, TG and De Vos, AJ (1999). Effect of breed of cattle on transmission rate and innate resistance to infection with Babesia bovis, Babesia bigemina transmitted by Boophilus microplus. Aust. Vet. J., 77: 461-464.
Bottero, V; Imbert, V; Frelin, C; Formento, JL and Peyron, JF (2003). Monitoring NF-kappa B transactivation potential via real-time PCR quantification of I kappa B-alpha gene expression. Mol. Diagn., 7: 187-194.
Chan, EKF; Nagaraj, SH and Reverter, A (2010). The evolution of tropical adaptation: comparing taurine and zebu cattle. Ani. Genet., 41: 467-477.
Creagh, EM and O’Neill, LA (2006). TLRs, NLRs and RLRs: a trinity of pathogen sensors that co-operate in innate immunity. Trends Immunol., 27: 352-357.
Cunningham, EP and Syrstad, O (1987). Crossbreeding bos indicus and bos taurus for milk production in the tropics. FAO Animal Production and Health Paper 68. FAO of the UN. ftp://ftp.fao.org/docrep/fao/009/t0095e/T0095E00.pdf.
Eskra, L; Mathison, A and Splitter, G (2003). Microarray analysis of mRNA levels from RAW264.7 macrophages infected with Brucella abortus. Infect. Immun., 71: 1125-1133.
Gruenheid, S; Canonne-Hergaux, F; Gauthier, S; Hackam, DJ; Grinstein, S and Gros, P (1995). The iron transport protein Nramp2 is an integral membrane glycoprotein that colocalizes with transferrin in recycling endosomes. J. Exp. Med., 189: 831-841.
Jiang, X and Baldwin, CL (1993). Effects of cytokines on intracellular growth of Brucella abortus. Infect. Immun. 61: 124-134.
Jiang, X; Leonard, B; Benson, R and Baldwin, CL (1993). Macrophage control of Brucella abortus: role of reactive oxygen intermediates and nitric oxide. Cell. Immunol., 151: 309-319.
Kawai, T and Akira, S (2010). The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat. Immunol., 11: 373-384.
Kirschning, CJ and Schumann, RR (2002). TLR2: cellular sensor for microbial and endogenous molecular patterns. Curr. Top. Microbiol. Immunol., 270: 121-144.
Ko, J; Gendron-Fitzpatrick, A; Ficht, TA and Splitter, GA (2002). Virulence criteria for Brucella abortus strains as determined by interferon regulatory factor-1 deficient mice. Infect. Immun., 70: 7004-7012.
Ko, J and Splitter, GA (2003). Molecular host-pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans. Clin. Microbiol. Rev., 6: 65-78.
Macedo, AA; Costaa, EA; Silva, APC; Paixão, TA and Santos, RL (2014). Monocyte-derived macrophages from Zebu (Bos taurus indicus) are more efficient to control Brucella abortus intracellular survival than macrophages from European cattle (Bos taurus taurus). Vet. Immunol. Immunopathol., 151: 294-302.
Macmicking, J; Xie, QW and Nathan, C (1997). Nitric oxide macrophage functions. Annu. Rev. Immunol., 15: 323-350.
Martínez, R; Dunner, S; Toro, R; Tobón, J; Gallego, J and Cãnón, J (2010). Effect of polymorphisms in the Slc11a1 coding region on resistance to brucellosis by macrophages in vitro and after challenge in two Bos breeds (Blanco Orejinegro and Zebu). Genet. Mol. Biol., 33: 463-470.
Miggin, SM and O’Neill, LA (2006). New insights into the regulation of TLR signaling. J. Leukoc. Biol., 80: 220-226.
Oliveira, SC; de Oliveira, FS; Macedo, GC; de Almeida, LA and Carvalho, NB (2008). The role of innate immune receptors in the control of Brucella abortus infection: toll-like receptors and beyond. Microbes Infect., 10: 1005-1009.
Oliveira, SC; Soeurt, N and Splitter, GA (2002). Molecular and cellular interactions between Brucella abortus antigens and host immune responses. Vet. Microbiol., 90: 417-424.
Parker, RJ; Shepherd, RK; Trueman, KF; Jones, GW; Kent, AS and Polkinghorne, IG (1985). Susceptibility of Bos indicus and Bos taurus to Anaplasma marginale and Babesia bigemina infections. Vet. Parasitol., 17: 205-213.
Pẽna, MT; Miller, JE; Wyatt, W and Kearney, MT (2000). Differences in susceptibility to gastrointestinal nematode infection between Angus and Brangus cattle in south Louisiana. Vet. Parasitol., 8: 51-61. Potempa, J and Pike, RN (2009). Corruption of innate immunity by bacterial proteases. J. Innate Immun., 1: 70-87.
Price, RE; Templeton, JW; Smith III, R and Adams, LG (1990). Ability of phagocytes from cattle naturally resistant or susceptible to brucellosis to control in vitro intracellular survival of Brucella abortus. Infect. Immun., 58: 879-886.
Qureshi, T; Templeton, JW and Adams, LG (1996). Intracellular survival of Brucella abortus, Mycobacterium bovis BCG. Salmonella Dublin and Salmonella typhimurium in macrophages from cattle genetically resistant to Brucella abortus. Vet. Immunol. Immunopathol., 50: 55-65.
Rechav, Y and Kostrzewski, MW (1991). Relative resistance of six cattle breeds to the tick Boophilus decoloratus in South Africa. Onderstepoort J. Vet. Res., 58: 181-186.
Splitter, G; Oliveira, SC; Carey, M; Miller, C; Ko, J and Covert, J (1996). T lymphocyte mediated protection against facultative intracellular bacteria. Vet. Immunol. Immunopathol., 54: 309-319.
Sun, YH; Hartigh, ABD; Santos, RL; Adams, LG and Tsolis, RM (2002). VirB-mediated survival of Brucella abortus in mice and macrophages is independent of a functional inducible nitric oxide synthase or macrophage NADPH oxidase in macrophages. Infect. Immun., 70: 4826-4832.
Sun, L; Song, Y; Riaz, H; Yang, H; Hua, G; Guo, A and Yang, L (2012). Polymorphisms in toll-like receptor 1 and 9 genes and their association with tuberculosis susceptibility in Chinese Holstein cattle. Vet. Immunol. Immunopathol., 147: 195-201.
Sun, KY; Xu, DH; Xie, C; Plummer, S; Tang, J; Yang, XF and Ji, XH (2017). Lactobacillus paracasei modulates LPS-induced inflammatory cytokine release by monocyte macrophages via the up-regulation of negative regulators of NF-kappaB signaling in aTLR2-dependent manner. Cytokine. 92: 1-11
Surendran, N; Hiltbold, EM; Heid, B; Akira, S; Standiford, TJ; Sriranganathan, N; Boyle, SM; Zimmerman, KL; Makris, MR and Witonsky, SG (2012). Role of TLRs in Brucella mediated murine DC activation in vitro and clearance of pulmonary infection in vivo. Vaccine. 30: 1502-1512.
Utech, KBW; Wharton, RH and Kerr, JD (1978). Resistance to Boophilus microplus (Canestrini) in different breeds of cattle. Aust. J. Agric. Res., 29: 885-895.
Wambura, PN; Gwakisa, PS; Silayo, RS and Rugaimukamu, EA (1998). Breed-associated resistance to tick infestation in Bos indicus and their crosses with Bos taurus. Vet. Parasitol., 77: 63-70.
Wang, M; Qureshi, N; Soeurt, N and Splitter, G (2001). High levels of nitric oxide production decrease early but increase late survival of Brucella abortus in macrophages. Microb. Pathol., 31: 221-230.
Yapan, S; Liping, S; Aizhen, G and Liguo, Y (2014). Effects of toll-like receptor 2 gene mutation on resistance to bovine brucellosis. Livest. Sci., 170: 30-34.
Zhan, Y; Liu, Z and Cheers, C (1996). Tumor necrosis factor alpha and interleukin 12 contribute to resistance to the intracellular bacterium Brucella abortus by different mechanisms. Infect. Immun., 64: 2782-2786. | ||
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