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Hemati, Zahra, Haghkhah, Masoud, Derakhshandeh, Abdollah, Singh, Shoor Vir, Kumar Chaubey, Kundan. (1397). Cloning and characterization of MAP2191 gene, a mammalian cell entry antigen of Mycobacterium avium subspecies paratuberculosis. , 7(4), 165-172. doi: 10.22099/mbrc.2018.30979.1354
Zahra Hemati; Masoud Haghkhah; Abdollah Derakhshandeh; Shoor Vir Singh; Kundan Kumar Chaubey. "Cloning and characterization of MAP2191 gene, a mammalian cell entry antigen of Mycobacterium avium subspecies paratuberculosis". , 7, 4, 1397, 165-172. doi: 10.22099/mbrc.2018.30979.1354
Hemati, Zahra, Haghkhah, Masoud, Derakhshandeh, Abdollah, Singh, Shoor Vir, Kumar Chaubey, Kundan. (1397). 'Cloning and characterization of MAP2191 gene, a mammalian cell entry antigen of Mycobacterium avium subspecies paratuberculosis', , 7(4), pp. 165-172. doi: 10.22099/mbrc.2018.30979.1354
Hemati, Zahra, Haghkhah, Masoud, Derakhshandeh, Abdollah, Singh, Shoor Vir, Kumar Chaubey, Kundan. Cloning and characterization of MAP2191 gene, a mammalian cell entry antigen of Mycobacterium avium subspecies paratuberculosis. , 1397; 7(4): 165-172. doi: 10.22099/mbrc.2018.30979.1354

Cloning and characterization of MAP2191 gene, a mammalian cell entry antigen of Mycobacterium avium subspecies paratuberculosis

Molecular Biology Research Communications
مقاله 2، دوره 7، شماره 4، 2018، صفحه 165-172    اصل مقاله (273.73 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2018.30979.1354
نویسندگان
Zahra Hemati1؛ Masoud Haghkhah* 1؛ Abdollah Derakhshandeh1؛ Shoor Vir Singh2؛ Kundan Kumar Chaubey2
1Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
2Department of Biotechnology, Institute of Applied Sciences and Humanities, GLA University, Chaumuhan, Mathura, UP, India 281406
چکیده
The aim of this study is to identify, clone and express a Mycobacterium avium subsp. paratuberculosis specific immunogenic antigen candidate, in order to develop better reagents for diagnosis and vaccines for the protection of the host. Therefore, MAP2191 gene (a member of MAPmce5 operon) from MAP, was isolated and characterized by Bioinformatics tools and in vitro experiments. Then, a novel Mce-whole protein encoded by MAP2191 gene was amplified and sub-cloned into E. coli. We tried to express the Mce/whole protein in different condition along with a positive expression control (pET28a-Mce/truncated plasmid that we know express well), to ensure that nothing is wrong regarding culture/induction condition. The level of the recombinant protein expression was analyzed by means of SDS-PAGE and Western blotting. Western blot analysis toward full-length MAP2191 protein and its truncation only demonstrated Mce/truncated protein. The concurrence of in-silico prediction of primary structure of MAP2191 protein results along with experimental results confirmed that expression of Mce/whole protein was affected by the hydrophobicity nature of this protein. Our data support the hypothesis that the presence of hydrophobic regions in protein structure can influence the level of recombinant protein expression. This stresses the importance of gene selection and the protein sequence checking of the hydrophobic content in any protein purification project in order to achieve a large amount of desirable proteins.
کلیدواژه‌ها
Johne's disease؛ Control؛ mce gene؛ MAP2191؛ Hydrophobicity
مراجع

1. Chaubey KK, Singh SV, Bhatia AK. ‘Indigenous’ and ‘Ethanol Vortex’ ELISA kits for diagnosis of Mycobacterium avium subsp. paratuberculosis infection in cattle: Is there a ‘globally relevant kit’ in the ‘Reverse Ice-burg’ environment? Indian J Exp Biol 2018;56:279-286.

2. Machado G, Kanankege K, Schumann V, Wells S, Perez A, Alvarez J. Identifying individual animal factors associated with Mycobacterium avium subsp. paratuberculosis (MAP) milk ELISA positivity in dairy cattle in the Midwest region of the United States. BMC Vet Res 2018;14:28.

3. Rathnaiah G, Zinniel DK, Bannantine JP, Stabel JR, Grohn YT, Collins MT, Barletta RG. Pathogenesis, Molecular genetics, and genomics of Mycobacterium avium subsp. paratuberculosis, the etiologic agent of Johne's disease. Front Vet Sci 2017;4:187.

4. Sahzad, Singh SV, Gupta S, Chaubey KK, Singh M, Dayal D, Dhama K, Hemati Z, Mansi S. Comparison of antibody response and direct microscopy between male and female goats in response to infection of Mycobacterium avium subspecies paratuberculosis in goatherds endemic for Johne’s disease. JSM Microbiol 2017;5:1035.

5. Rossi G, Grohn YT, Schukken YH, Smith RL. The effect of Mycobacterium avium ssp. Paratuberculosis infection on clinical mastitis occurrence in dairy cows. J Dairy Sci 2017; 100:7446-7454.

6. Hussain T, Zhao D, Shah SZA, Wang J, Yue R, Liao Y, Sabir N, Yang L, Zhou X. MicroRNA27a-3 pregulates antimicrobial responses of murine macrophages infected by Mycobacterium avium subspecies paratuberculosis by targeting interleukin-10 and TGF-β-activated protein kinase 1 binding protein 2. Front Immunol 2018;8:1915.

7. Singh M, Singh SV, Gupta S, Chaubey KK, Stephan BJ, Sohal JS, Dutta M.'Nano-immunetest' for the detection of live Mycobacterium avium subspecies paratuberculosis bacilli in the milk samples using magnetic nano-particles and chromogen. Vet Res Commun 2018; 42:183-194.

8. Gupta S, Singh SV, Gururaj K, Chaubey KK, Singh M, Lahiri B, Agarwal P, Kumar A, Misri J, Hemati Z, Bhatia AK. Comparison of IS900 PCR with ‘Taqman probe PCR’ and ‘SYBR Green Real time PCR’ assays in patients suffering with thyroid disorder and sero-positive for Mycobacterium avium subspecies paratuberculosis. Indian J Biotechnol 2017;228:234.

9. Kuenstner JT, Naser S, Chamberlin W, Borody T, Graham DY, McNees A, Hermon-Taylor J, Hermon-Taylor A, Dow CT, Thayer W, Biesecker J, Collins MT, Sechi LA, Singh SV, Zhang P, Shafran I, Weg S, Telega G, Rothstein R, Oken H, Schimpff S, Bach H, Bull T, Grant I, Ellingson J, Dahmen H, Lipton J, Gupta S, Chaubey K, Singh M, Agarwal P, Kumar A, Misri J, Sohal J, Dhama K, Hemati Z, Davis W, Hier M, Aitken J, Pierce E, Parrish N, Goldberg N, Kali M, Bendre S, Agrawal G, Baldassano R, Linn P, Sweeney RW, Fecteau M, Hofstaedter C, Potula R,  Timofeeva O, Geier S, John K, Zayanni N, Malaty HM, Kahlenborn C, Kravitz A, Bulfon A, Daskalopoulos G, Mitchell H, Neilan B, Timms V, Cossu D, Mameli G, Angermeier P, Jelic T, Goethe R, Juste RA, Kuenstner L. The Consensus from the Mycobacterium avium ssp. paratuberculosis (MAP) Conference 2017. Front Public Health 2017;5:208.

10. Dheenadhayalan V, Shin KS, Chang CF, Chang CD, Wang SJ, McDonough S, McDonough P, Stehman S, Shin S, Torres A, Chang YF. Cloning and characterization of the genes coding for antigen 85A, 85B and 85C of Mycobacterium avium subsp. paratuberculosis. DNA Seq 2002;13:287-294.

11. Okuni JB, Kateete DP, Okee M, Nanteza A, Joloba M, Ojok L. Application of antibodies to recombinant heat shock protein 70 in immune-histochemical diagnosis of Mycobacterium avium subspecies paratuberculosis in tissues of naturally infected cattle. Ir Vet J 2017; 270:10.

12. Mohn WW, vander-Geize R, Stewart GR, Okamoto S, Liu J, Dijkhuizen L, Eltis LD. The actinobacterial mce4 locus encodes a steroid transporter. J Biol Chem 2017;283:35368-35374.

13. Arruda S, Bomfim G, Knights R, Huimo-Byron T, Riley LW. Cloning of a M. tuberculosis DNA fragment associated with entry and survival inside cells. Science 1993;261:1454-1457.

14. Lu S, Tager LA, Chitale S, Riley LW. A cell-penetrating peptide derived from mammalian cell uptake protein of Mycobacterium tuberculosis. Anal Biochem 2006;353:7-14.

15. Takenami I, de Oliveira CC, Lima FR, Soares J, Machado A Jr, Riley LW, Arruda S. ImmunoglobulinG response to mammalian cell entry1A (Mce1A) protein as biomarker of active tuberculosis. Tuberculosis (Edinb) 2016;100:82-88.

16. Haile Y, Caugant DA, Bjune G, Wiker HG. Mycobacterium tuberculosis mammalian cell entry operon mce homologs in Mycobacterium other than tuberculosis MOTT. FEMS Immunol Med Microbiol 2002;332:125-132.

17. Garcia-Fernandez J, Papavinasasundaram K, Galán B, Sassetti CM, García JL. Molecular and functional analysis of the mce4 operon in Mycobacterium smegmatis. Environ Microbiol 2017;19:3689-699.

18. Lima FR, Takenami I, Cavalcanti MA, Riley LW, Arruda S. ELISA-based assay of immunoglobulinG antibodies against mammalian cell entry1A (Mce1A) protein: a novel diagnostic approach for leprosy. MemInst Oswaldo Cruz 2017;112:844-849.

19. Zhang Y, Li J, Li B, Wang J, Liu CH. Mycobacterium tuberculosis Mce3C promotes mycobacteria entry into macrophages through activation of β2 integrin-mediated signalling pathway. Cell Microbiol 2017;12:800.

20. Cangelosi GA, Do JS, Freeman R, Bennett JG, Semret M, Behr MA. The two-component regulatory system mtrAB is required for morphotypic multidrug resistance in Mycobacterium avium. Antimicrob Agents Chemother 2006;502:461-468.

21. Alexander DC, Turenne CY, Behr MA. Insertion and deletion events that define the pathogen Mycobacterium avium subsp. paratuberculosis. J Bacteriol 2009;913:1018-1025.

22. Semret M, Alexander DC, Turenne CY, de-Haas P, Overduin P, van Soolingen D, Cousins D, Behr MA. Genomic polymorphisms for Mycobacterium avium subsp. paratuberculosis diagnostics. J Clin Microbiol 2005;43:3704-3712.

23. Singh N, Singh SV, Gupta VK, Sharma VD, Sharma RK, Katoch VM. Isolation and identification of Mycobacterium paratuberculosis from naturally infected goatherds in India. Indian J Vet Pathol 1996;20:104-108.

24. Millar D, Ford J, Sanderson J, Withey S, Tizard M, Doran T, Hermon-Taylor J. IS900 PCR to detect Mycobacterium paratuberculosis in retail supplies of whole pasteurized cows’ milk in England and Wales. Appl Environ Microbiol 1996;62:3446-3452.

25. Sevilla I, Singh SV, Garrido JM, Aduriz G, Rodriguez S, Geijo MV, Cousins D, Behr MA. Molecular typing of Mycobacterium avium subspecies paratuberculosis strains from different hosts and regions. Rev Sci Techn 2005;24:1061-1066.

26. Whittington RJ, Marsh IB, Whitlock RH. Typing of IS1311 polymorphisms confirms that bison (Bison bison) with paratuberculosis in Montana are infected with strain of Mycobacterium avium subspecies paratuberculosis distinct from that occurring in cattle and other domestic livestock. Mole Cell Probes 2001;15:139-145.

27. Mukartal SY, Rathannama D, Narayanswamy HD, Gupta S, Chaubey KK, Singh M, Hemati Z, Nishanth C, Pachoori A, Dhama K, Singh SV. Assessment of Ovine Johne’s disease in the Mandya sheep breed in South India using multiple diagnostic tests and bio-typing of Mycobacterium avium subspecies paratuberculosis infection. Cogent Food Agric 2017;3: 1298391.

28. Rodriguez DC, Ocampo M, Varela Y, Curtidor H, Patarroyo MA, Patarroyo ME. Mce4F Mycobacterium tuberculosis protein peptides can inhibit invasion of human cell lines. Pathog Dis 2015;73:1-12

29. Castellanos E, Aranaz A, de Juan L, Dominguez L, Linedale R, Bull TJ. A 16 kb naturally occurring genomic deletion including mce and PPE genes in Mycobacterium avium subspecies paratuberculosis isolates from goats with Johne's disease. Vet Microbiol 2012; 159:60-68.

30. Sekar SC, Goswami PP, Deb R. Expression and purification of a gene encoding a 9.7 kDa PE protein of Mycobacterium avium subsp. paratuberculosis. 3 Biotech 2016;6:198.

31. Rosano GL, Ceccarelli EA. Recombinant protein expression in Escherichia coli: advances and challenges. Front Microbiol 2014;5:172.

32. Cho D, Shin SJ, Talaat AM, Collins MT. Cloning, expression, purification and sero-diagnostic evaluation of fourteen Mycobacterium paratuberculosis proteins. Protein Expr Purif 2007;53:411-420.

33. Strych U, Penland RL, Jimenez M, Krause KL, Benedik MJ. Characterization of the alanineracemases from two mycobacteria. FEMS Microbiol Lett 2001;196:93-98.

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