| تعداد نشریات | 24 |
| تعداد شمارهها | 917 |
| تعداد مقالات | 8,230 |
| تعداد مشاهده مقاله | 16,274,423 |
| تعداد دریافت فایل اصل مقاله | 13,750,855 |
Activating natural killer (NK) cytotoxicity of canine CD5−CD21− cells requires low surface CD5 density NK cells | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 3، دوره 19، شماره 2 - شماره پیاپی 63، 2018، صفحه 87-95 اصل مقاله (1.2 M) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2018.4851 | ||
| نویسندگان | ||
| C.S. Lin1، 2؛ C.P. Chang1؛ H. C. Chiang1، 2؛ T. F. Chuang2؛ C. H. Hsu1؛ C. C. Liu* 1 | ||
| 1Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan | ||
| 2Animal Cancer Center, College of Bio-Resources and Agriculture, National Taiwan University, Taipei, Taiwan | ||
| چکیده | ||
| Natural killer (NK) cells play a crucial role in regulating immune functions. Few studies have characterized canine NK cells. We previously demonstrated that canine peripheral blood lymphocytes (PBLs) with a low surface CD5 density (CD5lo) are considered a critical NK population. Natural killer cells in most mammals do not express T-cell markers, but canine CD5lo cells express surface molecules, such as CD3 T-cell receptors. These features make canines unique models for the study of comparative immunology in NK cells. In this study, we discovered that CD5lo and CD21 double-negative (CD5lo-ne/CD2−) cells were originally low in NK cytotoxicity and their NK cytotoxicity was highly activated when co-cultured with CD5lo NK cells. The cytotoxicity was not activated when co-cultured with other cell types, such as high surface CD5 density (CD5hi) cells. The CD5lo-negative (CD5lo-ne) population comprises CD5− and CD5hi cells. CD5-cells were low in NK cytotoxicity initially or after culturing with interleukin-2 (IL-2) without CD5lo cells; however, the addition of CD5lo cells in a similar medium markedly enhanced the NK activity. By contrast, CD5hi cells were always NK inactive, irrespective of them being cultured with CD5lo cells or not. We further verified that only the CD5−CD21− cells, which were separated from CD5−CD21+ cells in the entire CD5− population, showed activated NK activity through CD5lo cell induction. This study is the first to reveal that canine NK cells enhanced NK-inert cells to become NK-cytotoxic cells. Additionally, it is concluded that in beagles, except for CD5lo cells, CD5−CD21− cells show NK activity. | ||
| کلیدواژهها | ||
| CD5−CD21− cells؛ Cytotoxicity؛ Low surface CD5 density؛ Natural killer cells | ||
| مراجع | ||
|
Biron, CA (2010). More things in heaven and earth: defining innate and adaptive immunity. Nat. Immunol., 11: 1080-1082. Brudno, JN; Somerville, RP; Shi, V; Rose, JJ; Halverson, DC; Fowler, DH; Gea-Banacloche, JC; Pavletic, SZ; Hickstein, DD; Lu, TL; Feldman, SA; Iwamoto, AT; Hansen, BG; Blacklock-Schuver, B; Hakim, FT; Rosenberg, SA; Gress, RE and Kochenderfer, JN (2016). Allogeneic T cells that express an anti-CD19 chimeric antigen receptor induce remissions of B-cell malignancies that progress after allogeneic hematopoietic stem-cell transplantation without causing graft-versus-host disease. J. Clin. Oncol., 34: 1112-1121. Chiang, HC; Liao, AT; Jan, TR; Wang, YS; Lei, HJ; Tsai, MH; Chen, MF; Lee, CY; Lin, YC; Chu, RM and Lin, CS (2013). Gene-expression profiling to identify genes related to spontaneous tumor regression in a canine cancer model. Vet. Immunol. Immunopathol., 151: 207-216. Christaki, E; Diza, E; Giamarellos-Bourboulis, EJ; Papadopoulou, N; Pistiki, A; Droggiti, DI; Georgitsi, M; Machova, A; Lambrelli, D; Malisiovas, N; Nikolaidis, P and Opal, SM (2015). NK and NKT cell depletion alters the outcome of experimental pneumococcal pneumonia: relationship with regulation of interferon-γ production. J. Immunol., Article ID 532717. Denecke, C; Yuan, X; Ge, X; Kim, IK; Bedi, D; Boenisch, O; Weiland, A; Jurisch, A; Kotsch, K; Pratschke, J; Reutzel-Selke, A and Tullius, SG (2013). Synergistic effects of prolonged warm ischemia and donor age on the immune response following donation after cardiac death kidney transplantation. Surgery. 153: 249-261. Duggan, MC; Campbell, AR; McMichael, EL; Opheim, KS; Levine, KM; Bhave, N; Culbertson, MC; Noel, T; Yu, Li and Carson, WE (2017). Co-stimulation of the fc receptor and interleukin-12 receptor on human natural killer cells leads to increased expression of cd25. Oncoimmunology. 16: 7(2): e1381813. Gerner, W; Kaser, T and Saalmuller, A (2009). Porcine T lymphocytes and NK cells--an update. Dev. Comp. Immunol., 33: 310-320. Hsiao, YW; Liao, KW; Hung, SW and Chu, RM (2004). Tumor-infiltrating lymphocyte secretion of IL-6 antagonizes tumor-derived TGF-beta 1 and restores the lymphokine-activated killing activity. J. Immunol., 172: 1508-1514. Huang, YC; Hung, SW; Jan, TR; Liao, KW; Cheng, CH; Wang, YS and Chu, RM (2008). CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells. J. Leukocyte Biol., 84: 1501-1510. Knapp, DW; Leibnitz, RR; DeNicola, DB; Turek, JJ; Teclaw, R; Shaffer, L and Chan, TC (1993). Measurement of NK activity in effector cells purified from canine peripheral lymphocytes. Vet. Immunol. Immunopathol., 35: 239-251. Knapp, DW; Turek, JJ; DeNicola, DB; Chan, TC; Carter, WO; Snyder, PW and Robinson, JP (1995). Ultra-structure and cytochemical staining characteristics of canine natural killer cells. Anat. Rec., 243: 509-515. Macêdo, AA; Marciano, AP; Rocha, LM; Alves-Júnior, JR; Faria, AM; Bittar, JF; Araújo, MS; Santos, RL and Martins-Filho, OA (2013). Comparative phenotypic profile of subpopulations of peripheral blood leukocytes in European (Bos taurus taurus) and Zebu cattle (Bos taurus indicus). Genet. Mol. Res., 12: 6838-6849. Mathew, S; Kim, J and Mathew, P (2014). Characterization of human CS1 gene promoter in NK and B cells. J. Immunol., 126: 10. Michael, HT; Ito, D; Cullar, VC; Zhang, B; Miller, JS and Modiano, JF (2013). Isolation and characterization of canine natural killer cells. Vet. Immunol. Immunop., 155: 211-217. Nariai Nakada, Y; Nariai, K; Kosaka, T; Kuwabara, M and Kiuchi, Y (1999). Morphological observation of canine Odeberg, J; Browne, H; Metkar, S; Froelich, CJ; Branden, L; Cosman, D and Soderberg-Naucler, C (2003). The human cytomegalovirus protein UL16 mediates increased resistance to natural killer cell cytotoxicity through resistance to cytolytic proteins. J. Virol., 77: 4539-4545. Orange, JS (2006). Human natural killer cell deficiencies. Curr. Opin Allergy Clin. Immunol., 6: 399-409. Ribatti, D (2017). Historical overview on the morphological characterization of large granular lymphocytes/natural killer cells. Immunol. Lett., 190: 58-63. Roquilly, A; Broquet, A; Jacqueline, C; Masson, DP; Segain, JP; Braudeau, C; Vourc’h, M; Caillon, JP; Altare, F; Josien, R; Retière, C; Villadangos, J and Asehnoune, K (2014). Hydrocortisone prevents immunosuppression by interleukin-10+ natural killer cells after trauma-hemorrhage. Crit. Care Med., 42: e752-e761. Shin, DJ; Park, JY; Jang, YY; Lee, JJ; Lee, YK; Shin, MG; Jung, JY; Carson, WE 3rd; Cho, D and Kim, SK (2013). Ex vivo expansion of canine cytotoxic large granular lymphocytes exhibiting characteristics of natural killer cells. Vet. Immunol. Immunopathol., 153: 249-259. Stojanovic, A; Fiegler, N; Brunner-Weinzierl, M and Cerwenka, A (2014). CTLA-4 is expressed by activated mouse NK cells and inhibits NK cell IFN-γ production in response to mature dendritic cells. J. Immunol., 192: 4184-4191. Talleur, AC; Triplett, BM; Federico, S; Mamcarz, E; Janssen, W; Wu, W; Shook, D; Leung, W and Furman, WL (2017). Consolidation therapy for newly diagnosed pediatric patients with high-risk neuroblastoma using busulfan/melphalan, autologous hematopoietic cell transplantation, anti-GD2 antibody, granulocyte-macrophage colony-stimulating factor, interleukin-2, and haploidentical natural killer cells. Biol. Blood Marrow Tr., 23: 1910-1917. Vankayalapati, R; Klucar, P; Wizel, B; Weis, SE; Samten, B; Safi, H; Shams, H and Barnes, PF (2004). NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen. J. Immunol., 172: 130-137. | ||
|
آمار تعداد مشاهده مقاله: 1,699 تعداد دریافت فایل اصل مقاله: 623 |
||