| تعداد نشریات | 24 |
| تعداد شماره‌ها | 929 |
| تعداد مقالات | 8,346 |
| تعداد مشاهده مقاله | 16,804,190 |
| تعداد دریافت فایل اصل مقاله | 14,118,139 |
Pathogenicity characteristics of Enterococcus faecium from diseased black bears | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 2، دوره 19، شماره 2 - شماره پیاپی 63، 2018، صفحه 82-86 اصل مقاله (456.42 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2018.4850 | ||
| نویسندگان | ||
| F. Dai1؛ X. Xiang1؛ G. Duan1؛ B. Duan2؛ X. Xiao1؛ H. Chang* 1 | ||
| 1Department of Clinical Veterinary, College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan, 650201, China | ||
| 2Animal Disease Prevention and Control Center of Yunnan Province, Kunming, Yunnan, 650201, China | ||
| چکیده | ||
| The death of several black bears at the black bear breeding base in Yunnan Pingbian Daweishan is a matter of concern. Multiple black bears exhibited decreased appetite or unusual waste, and some were soporific or suffered from vomiting and anhelation. In order to ascertain the cause of death, 16S rDNA gene sequencing and phylogenetic analysis was performed on bacteria isolated from tissue samples obtained from dead bears. The biochemical characteristics of the isolated bacteria were subsequently analyzed using different biochemical test systems. The bacteria can decompose glucose, but it cannot produce gas. The fermentation study of sucrose, lactose, trehalose, glycerol and mannitol yielded positive results; while it was unable to decompose urea or ODC (ornithine decarboxylase). Basic Local Alignment Search Tool (BLAST) analysis of a ~1500-bp DNA product amplified from the 16S rDNA of the bacterial isolate revealed that Enterococcus faecium from black bears is highly similar to other E. faecium isolates in the National Center for Biotechnology Information (NCBI) database, and the highest sequence similarity (99%) was with the reference strain. In addition, mice infected with the E. faecium isolate succumbed to severe damage to the lungs, liver, spleen, myocardium, and kidney tissues. In summary, the isolated E. faecium from dead black bears induced pathological changes in mice. | ||
| کلیدواژه‌ها | ||
| Black bear؛ Characterization؛ Enterococcus faecium؛ Isolation | ||
| مراجع | ||
|
Abeijón, MC;Medina, RB;Katz, MB and González, SN (2006). Technological properties of Enterococcus faecium isolated from ewe’s milk and cheese with importance for flavour development. Can. J. Microbiol., 52: 237-245.
Abriouel, H;Lucas, R; Ben, ON;Valdivia, E;Maqueda, M;Martínez-Cañamero, M andGálvez, A (2005). Enterocin AS-48RJ: a variant of enterocin AS-48 chromosomally encoded by Enterococcus faecium RJ16 isolated from food. Syst. Appl. Microbiol., 28: 383-397.
Belgacem, ZB; Abriouel, H; Omar, NB;Lucasb, R;Martínez-Canamerob, M;Gálvezb, A and Manaia, M (2009). Antimicrobial activity, safety aspects and some technological properties of bacteriocinogenic Enterococcus faecium from artisanal Tunisian fermented meat. Food Contr., 21: 462-470.
Cocolin, L; Foschino, R; Comi, G and Grazia, FM (2007). Description of the bacteriocins produced by two strains of Enterococcus faecium isolated from Italian goat milk. Food Microbiol., 24: 752-758.
Fisher, K and Phillips, C (2009). The ecology, epidemiology and virulence of Enterococcus. Microbiology. 155: 1749-1757.
Garcia-Migura, L;Liebana, E and Jensen, LB (2007). Transposon characterization of vancomycin-resistant Enterococcus faecium (VREF) and dissemination of resistance associated with transferable plasmids. J. Antimicrob. Chemother., 60: 263-268.
Izquierdo, E; Bednarczyk, A;Schaeffer, C;Cai, Y;Marchioni, E;Van, DAand Ennahar, S (2008). Production of enterocin L50A, L50B, and IT, a new enterocin, by Enterococcus faecium IT62, a strain isolated from Italian ryegrass in Japan. Antimicrob. Agents Chemother., 52: 1917-1923.
Koch, S;Hufnagel, M;Theilacker, Cand Huebner, J (2004). Enterococcal infections: host response, therapeutic, and prophylactic possibilities. Vaccine. 22: 822-830.
Leavis, HL; Willems, RJ;van, WWJ;Schuren, FH; Caspers, MP and Bonten, MJ (2007). Insertion sequence-driven diversification creates a globally dispersed emerging multiresistant subspecies of E-faecium. PLoS Pathog., 3: e7.
Liu, G; Griffiths, MW; Wu, P; Wang, H; Zhang, X and Li, P (2011). Enterococcus faecium LM-2, a multi-bacteriocinogenic strain naturally occurring in “Byaslag”, a traditional cheese of inner Mongolia in China. Food Contr., 22: 283-289.
Lleò, MM;Bonato, B;Benedetti, D and Canepari, P (2005). Survival of enterococcal species in aquatic environments. FEMS Microbiol. Ecol., 54: 189-196.
Palmer, KL;Kos, VN and Gilmore, MS (2010). Horizontal gene transfer and the genomics of enterococcal antibiotic resistance. Curr. Opin. Microbiol., 13: 632-639.
Petersen, A and Dalsgaard, A (2003). Antimicrobial resistance of intestinal Aeromonas spp. and Enterococcus spp. in fish cultured in integrated broiler-fish farms in Thailand. Aquaculture. 219: 71-82.
Sood, S; Malhotra, M; Das, BK andKapil, A (2012). Enterococcus in wound infections: virulence and antimicrobial resistance. Acta Microbiol. Immunol. Hung., 59: 263-269.
Strompfová, V and Lauková, A (2007). In vitro study on bacteriocin production of enterococci associated with chickens. Anaerobe. 13: 228-237.
Wilson, IG andMcAfee, GG (2002). Vancomycin-resistant enterococci in shellfish, unchlorinated waters, and chicken. Int. J. Food Microbiol., 79: 143-151.
Yoon, MY; Kim, YJ and Hwang, HJ (2008). Properties and safety aspects of Enterococcus faecium strains isolated from Chungkukjang, a fermented soy product. Food Sci. Technol., 41: 925-933. | ||
|
آمار تعداد مشاهده مقاله: 1,005 تعداد دریافت فایل اصل مقاله: 754 |
||