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Induction of apoptosis and necrosis in human acute erythroleukemia cells by inhibition of long non-coding RNA PVT1 | ||
| Molecular Biology Research Communications | ||
| مقاله 6، دوره 7، شماره 2، 2018، صفحه 89-96 اصل مقاله (390.94 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2018.29081.1316 | ||
| نویسندگان | ||
| Mahsa Salehi؛ Mohammadreza Sharifi* | ||
| Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| چکیده | ||
| Recent advances in molecular medicine have proposed new therapeutic strategies for cancer. One of the molecular research lines for the diagnosis and treatment of cancer is the use of long non-coding RNAs (LncRNAs) which are a class of non-coding RNA molecules longer than 200 base pairs in length that act as the key regulator of gene expression. Different aspects of cellular activities like cell growth, proliferation, differentiation, apoptosis and migration are regulated by lncRNAs. In various cancers, aberrant expression of lncRNAs has been reported. One of the lncRNAs that showed upregulation in human acute myeloid leukemia (AML) is lncRNA plasmacytoma variant translocation 1 (PVT1). Here, we performed blockage of lncRNA PVT1 in human acute erythroleukemia (AEL) cell line (KG1) using antisense LNA GapmeRs. Then, at different time points (24, 48 and 72 hours) after transfection, qRT‑real‑time PCR and Annexin‑V/Propidium Iodide staining assay were performed. The data were processed using the ANOVA test. At all three time points, the ratio of apoptotic cells in the PVT1 antisense LNA GapmeRs treated group was higher than the other groups. The ratio of necrotic cells in the antisense LNA GapmeRs group was also higher than the other groups. These assessments show that inhibition of lncRNA PVT1 could significantly induce apoptosis and necrosis in KG1 cells. Our findings can be used in translational medicine for future investigation in acute erythroleukemia and treatment approach based on antisense therapy. | ||
| کلیدواژهها | ||
| Long non-coding RNA؛ LncRNA PVT1؛ Apoptosis؛ Acute erythroleukemia | ||
| مراجع | ||
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1. Fang Y, Fullwood MJ. Roles, function, and mechanisms of long non-coding RNAs in cancer. Genomics Proteomics Bioinf 2016;14:42-54. 2. Bartonicek N, Maag JLV, Dinger ME. Long noncoding RNAs in cancer: mechanisms of action and technological advancements. Mol Cancer 2016;15:43. 3. Rodríguez-Malavé NI, Rao DS. Long noncoding RNAs in hematopoietic malignancies. Briefings Funct Genomics 2016;15:227-238. 4. Harries Lorna W. Long non-coding RNAs and human disease. Biochem Soc Trans 2012;40: 902-906. 5. Jalali F, Sharifi M, Salehi R. Kefirinduces apoptosis and inhibits cell proliferation in human acute erythroleukemia. Med Oncol 2016;33:7. 6. Zuo Z, Polski JM, Kasyan A, Medeiros LJ. Acute erythroid leukemia. Arch Pathol Lab Med 2010;134:1261-1270. 7. Santos FP, Bueso-Ramos CE, Ravandi F. Acute erythroleukemia: diagnosis and management. Expert Rev Hematol 2010;3:705-718. 8. Wei S, Wang K. Long noncoding RNAs: pivotal regulators in acute myeloid leukemia. Exp Hematol Oncol 2016;5:30. 9. Zeng C, Yu X, Lai J, Yang L, Chen S, Li Y. Overexpression of the long non-coding RNA PVT1 is correlated with leukemic cell proliferation in acute promyelocytic leukemia. J Hematol Oncol 2015;8:126. 10. Graham M. Adams JM. Chromosome 8 breakpoint far 3' of the c-myc oncogene in a Burkitt's lymphoma 2;8 variant translocation is equivalent to the murine pvt-1 locus. EMBO J 1986;5:2845-2851. 11. Zhuang C, Li J, Liu Y, Chen M, Yuan J, Fu X, Zhan Y, Liu L, Lin J, Zhou Q. Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells. Oncotarget 2015;6:41194. 12. Ding J, Li D, Gong M, Wang J, Huang X, Wu T, Wang C. Expression and clinical significance of the long non-coding RNA PVT1 in human gastric cancer. OncoTargets Ther 2014;7:1625. 13. Takahashi Y, Sawada G, Kurashige J, Uchi R, Matsumura T, Ueo H, Takano Y, Eguchi H, Sudo T, Sugimachi K. Amplification of PVT-1 is involved in poor prognosis via apoptosis inhibition in colorectal cancers. Br J Cancer 2014;110:164. 14. Guan Y, Kuo W-L, Stilwell JL, Takano H, Lapuk AV, Fridlyand J, Mao J-H, Yu M, Miller MA, Santos JL. Amplification of PVT1 contributes to the pathophysiology of ovarian and breast cancer. Clin Cancer Res 2007;13:5745-5755. 15. Colombo T, Farina L, Macino G, Paci P. PVT1: a rising star among oncogenic long noncoding RNAs. BioMed Res Int2015:304208 16. Parasramka MA, Maji S, Matsuda A, Yan IK, Patel T. Long non-coding RNAs as novel targets for therapy in hepatocellular carcinoa. Pharmacol Ther 2016;161:67-78. 17. Lai J, Ozen A, Mouritzen P, Tolstrup N, Frandsen NM. Abstract PR14: Potent knock down of lncRNAs in vitro and in vivo with antisense LNA™ GapmeRs. Cancer Res 2016; 76:PR14. 18. Cui M, You L, Ren X, Zhao W, Liao Q, Zhao Y. Long non-coding RNA PVT1 and cancer. Biochem Biophys Res Commun 2016;471:10-14. 19. Saultz JN, Garzon R. Acute myeloid leukemia: A Concise Review. J Clin Med 2016;5:33. 20. Molina CA, Rodríguez MJR, de Marcos NS, Font P. Acute myeloblastic leukemia. Adv Ther 2011;28:10. 21. Huang C, Liu S, Wang H, Zhang Z, Yang Q, Gao F. LncRNA PVT1 overexpression is a poor prognostic biomarker and regulates migration and invasion in small cell lung cancer. Am J Transl Res 2016;8:5025-5034. 22. Pickard MR, Williams GT. Targeting long non-coding RNAs (lncRNAs) with oligonucleotides in cancer therapy. Transl Cancer Res 2016;5:S926-S927. 23. Wong RSY. Apoptosis in cancer: from pathogenesis to treatment. J Exp Clin Cancer Res 2011;30:87. 24. Sakamoto KM, Grant S, Saleiro D, Crispino JD, Hijiya N, Giles F, Platanias L, Eklund EA. Targeting novel signaling pathways for resistant acute myeloid leukemia. Mol Genet Metab 2015;114:397-402 25. Li J, Meng H, Bai Y, Wang K. Regulation of lncRNA and its role in cancer metastasis. Oncol Res 2016;23:205-217. | ||
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