| تعداد نشریات | 24 |
| تعداد شمارهها | 886 |
| تعداد مقالات | 7,900 |
| تعداد مشاهده مقاله | 14,905,373 |
| تعداد دریافت فایل اصل مقاله | 12,668,139 |
Characterization of dengue virus in Aedes aegypti and Aedes albopictus spp. of mosquitoes: A study in Khyber Pakhtunkhwa, Pakistan | ||
| Molecular Biology Research Communications | ||
| مقاله 4، دوره 7، شماره 2، 2018، صفحه 77-82 اصل مقاله (128.03 K) | ||
| نوع مقاله: Short communication | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2018.29073.1315 | ||
| نویسندگان | ||
| Mubbashir Hussain* 1؛ Shahzad Munir2؛ Kashif Rahim3؛ Nawaz Haider Bashir2؛ Abdul Basit1؛ Baharullah Khattak1 | ||
| 1Vector Borne Diseases Lab, Department of Microbiology, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, 26000 Pakistan | ||
| 2Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan, China | ||
| 3Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing 100875, China | ||
| چکیده | ||
| Dengue is a vector-borne disease caused by dengue virus. According to the recent report of CDC that one-third population of the world are at high risk with Dengue fever. The prevalence of the dengue hemorrhagic fever was found more in tropical and sub-tropical regions of the world. Aedes mosquitoes was reported as the main cause of transmission of dengue virus. So the current study was planned to characterize the virus in Aedes mosquitoes collected from different area of Pakistan. In current investigation, Aedes mosquitoes and larvae were trapped under conducive conditions which are counted as 495 Aedes mosquitoes and 260 Aedes larvae. First of all, adult mosquitoes were identified morphologically under microscopy, counted as 73.3% Ae. aegypti and 26.7% Ae. albopictus. Finally, reverse transcriptase polymerase chain reaction analyses that only 4 adults of Aedes mosquitoes and 10 Aedes larvae as naturally infected with dengue virus with possible source Ae. aegypti. This study basically uncovered the presence of virus in different species of mosquitoes in southern regions of Pakistan. The present study will also give us an insight for vector control programs of dengue virus in the affected area. | ||
| کلیدواژهها | ||
| Dengue Virus؛ Aedes mosquitoes؛ Khyber Pakhtunkhwa Pakistan | ||
| مراجع | ||
|
1. Alkenani NA. Influence of the mixtures composed of slow–release insecticide formulations against Aedes aegypti mosquito larvae reared in pond water. Saudi J Biol Sci 2017;24:1181-1185. 2. Agramonte NM, Bloomquist JR, Bernier UR. Pyrethroid resistance alters the blood-feeding behavior in Puerto Rican Aedes aegypti mosquitoes exposed to treated fabric. PLoS Negl Trop Dis 2017;11:e0005954. 3. Hasan Z, Razzak S, Farhan M, Rahim M, Islam N, Samreen A, Khan E. Increasing usage of rapid diagnostics for dengue virus detection in Pakistan. J Pak Med Assoc 2017;67:548. 4. Tsujimoto H, Hanley KA, Sundararajan A, Devitt NP, Schilkey FD, Hansen IA. Dengue virus serotype 2 infection alters midgut and carcass gene expression in the Asian tiger mosquito, Aedes albopictus. PloS One 2017;12:e0171345. 5. Doosti S, Yaghoobi-Ershadi MR, Schaffner F, Moosa-Kazemi SH, Akbarzadeh K, Gooya MM, Vatandoost H, Shirzadi MR, Mosta-Favi E. Mosquito surveillance and the first record of the invasive mosquito species Aedes (Stegomyia) albopictus (Skuse)(Diptera: Culicidae) in southern Iran. Iran J Public Health 2016;45:1064. 6. Agarwal K, Malik S, Mittal P. A retrospective analysis of the symptoms and course of dengue infection during pregnancy. Int J Gynaecol Obstet 2017;139:4-8. 7. Jahan F. Dengue fever (DF) in Pakistan. Asia Pac Fam Med 2011;10:1. 8. Khan E, Kisat M, Khan N, Nasir A, Ayub S, Hasan R. Demographic and clinical features of dengue fever in Pakistan from 2003-2007: a retrospective cross-sectional study. PLoS One 2010;5:e12505. 9. Ahmed A, Tanveer M, Khan GM, Imran M. Dengue fever again in Pakistan: are we going in the right direction?. Public Health 2017;152:153-6. 10. Arslan A, Rathor HR, Mukhtar MU, Mushtaq S, Bhatti A, Asif M, Arshad I, Ahmad JF. Spatial distribution and insecticide susceptibility status of Aedes aegypti and Aedes albopictus in dengue affected urban areas of Rawalpindi, Pakistan. J Vector Borne Dis 2016;53:136. 11. Itrat A, Khan A, Javaid S, Kamal M, Khan H, Javed S, Kalia S, Khan AH, Sethi MI, Jehan I. Knowledge, awareness and practices regarding dengue fever among the adult population of dengue hit cosmopolitan. PLoS One 2008;3:e2620. 12. Suleman M, Faryal R, Alam MM, Sharif S, Shaukat S, Aamir UB, Khurshid A, Angez M, Umair M, Sufian MM, Arshad Y. Dengue virus serotypes circulating in Khyber Pakhtunkhwa Province, Pakistan, 2013-2015. Ann Lab Med 2017;37:151-154. 13. Mukhtar M, Tahir Z, Baloch TM, Mansoor F, Kamran J. Entomological investigations of dengue vectors in epidemic-prone districts of Pakistan during 2006-2010.WHO Reginal Office for South-East Asia. http://www.who.int/iris/handle/10665/171002. 14. Nasiruddin M. A note on the natural infectivity of mosquitoes in East Pakistan. Pak J Health 1952;2:113-5. 15. Huang YM. Medical entomology studies-XI. The subgenus Stegomyia of Aedes in the Oriental region with keys to the species (Diptera: Culicidae). SMITHSONIAN INSTITUTION WASHINGTON DC; 1979. 16. Kanthong N, Khemnu N, Sriurairatana S, Pattanakitsakul SN, Malasit P, Flegel TW. Mosquito cells accommodate balanced, persistent co-infections with a densovirus and Dengue virus. Dev Comp Immunol 2008;32:1063-75. 17. Joshi V, Mourya DT, Sharma RC. Persistence of dengue-3 virus through transovarial transmission passage in successive generations of Aedes aegypti mosquitoes. The Am J Trop Med Hyg. 2002;67:158-61. 18. Cruz EI, Salazar FV, Porras E, Mercado R, Orais V, Bunyi J. Entomological survey of dengue vectors as basis for developing vector control measures in Barangay Poblacion, Muntinlupa City, Philippines, 2008. 19. Khan J, Munir W, Khan BT, Ahmad Z, Shams WA, Khan A. Dengue outbreak 2013: Clinical profile of patients presenting at DHQ Burner and THQ Shangla, Khyber Pakhtunkhwa, Pakistan. Immunity Diseases 2015;3:a11. 20. Scott TW, Amerasinghe PH, Morrison AC, Lorenz LH, Clark GG, Strickman D, Kittayapong P, Edman JD. Longitudinal studies of Aedes aegypti (Diptera: Culicidae) in Thailand and Puerto Rico: blood feeding frequency. J Med Entomol 2000;37:89-101. | ||
|
آمار تعداد مشاهده مقاله: 2,398 تعداد دریافت فایل اصل مقاله: 2,556 |
||