| تعداد نشریات | 24 |
| تعداد شمارهها | 852 |
| تعداد مقالات | 7,575 |
| تعداد مشاهده مقاله | 13,420,803 |
| تعداد دریافت فایل اصل مقاله | 11,583,431 |
Genetic variations among three major ethnic groups in Nigeria using RAPD | ||
| Molecular Biology Research Communications | ||
| مقاله 1، دوره 7، شماره 2، 2018، صفحه 51-58 اصل مقاله (164.28 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2018.26098.1280 | ||
| نویسندگان | ||
| Olukanni A. Titilayo1؛ Amoo O. Samuel2؛ Olukanni O. David* 3؛ Taiwo I. Adewunmi1 | ||
| 1Department of Cell Biology and Genetics, University of Lagos. P.M.B. 56, Akoka, Lagos State, Nigeria Nigeria | ||
| 2Human Virology Laboratory, Nigerian Institute of Medical Research, P.M.B 2013, Yaba, Lagos, Nigeria | ||
| 3Department of Chemical Sciences (Biochemistry), Redeemer’s University. P.M.B. 230, Ede, Osun State, Nigeria | ||
| چکیده | ||
| Genetically, every individual is unique; this may stem from inheritance, geographical locations, and/or environmental interactions. This study examined the possibility of developing a cheap and easy-to-use marker that can distinguish among the three ethnic groups in Nigeria using RAPD-PCR. Five RAPD primers, OPA1-3 and OPC1-2, were randomly selected and used to amplify DNA samples isolated from blood of eighteen human subjects representing the three major ethnic groups in Nigeria (six subjects each). Genomic DNAs were extracted using DNA isolation kit, RAPD-PCR amplification was performed and gel electrophoresis was done. Genetic similarity between the band polymorphism was evaluated as frequencies of occurrence and the phylogenetic tree constructed. Three of the five primers show various polymorphisms; the highest frequency band for primer OPA1 is 50% while that of primer OPA2 is 100% and for OPC2 is 83.33%. Although OPA2 has common bands in majority of the samples few of the bands are ethnic group specific. Bands 471 and 435 bp are specific for the Hausa ethnic group at 66.67% frequency. Similarly, in primer OPC2, band 320 can be used to distinguish the Hausas from the other two ethnic groups. Analysis of variance (ANOVA) and test for homogeneity showed that there is no significant difference in the polymorphism between and among the groups. In conclusion this research has given an insight into the possibility of developing RAPD primers that could be used to distinguish people of different ethnic groups. | ||
| کلیدواژهها | ||
| Nigerian ethnic؛ RAPD-PCR؛ Genetic Variation؛ PyElph؛ Phylogeny | ||
| مراجع | ||
|
1. Erskine W, Muehlbauer FJ. Allozyme and morphological variability, out crossing rate and core collection formation in lentil germplasm. Theor Appl Genet 1991;83:119-125 2. Kumar P, Gupta VK, Misra AK, Modi DR, Pandey BK. Potential of molecular markers in plant biotechnology. Plant Omics 2009;2:141-162. 3. Bennett JS, Jolley KA, Earle SG, Corton C, Bentley SD, Parkhill J, Maiden MCJ. A genomic approach to bacterial taxonomy: an examination and proposed reclassification of species within the genus Neisseria. Microbiology 2012;158:1570-1580. 4. Kumar NS, Gurusubramanian G. Random amplified polymorphic DNA (RAPD) markers and its applications. “National Level Workshop on Random Amplified Polymorphic DNA (RAPD) markers and it’s Applications.” Sci Vision 2011;11:116-124. 5. Williams JG, Kbelik AR, Livak KJ, Rafalsk JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990;18:6531-6535. 6. Mbwana J, Bolin L, Lyamuya E, Mhalu F, Lagergard T. Molecular characterization of Haemophilus ducreyi isolates from different geographical locations. J Clinical Microbiol 2006;44:132-137. 7. Nur-Nazifah M, Sabri MY, Zamri-Saad M, Siti-Zahrah A, Firdaus-Nawi M. Random amplified polymorphic DNA (RAPD): a powerful method to differentiate Streptococcus agalactiae strains. In Symposia Proceeding of Diseases in Asian Aquaculture VII ed. Bondad-Reantaso, MG, Jones, JB, Corsin, F and Aoki T. 2011; pp. 29-38. Selangor, Malaysia: Fish Health Section, Asian Fisheries Society. 8. Arif IA, Bakir MA, Khan HA, Alfarhan AH, Al Homaidan AA, Bahkali AH, Alsadoon M, Shobrak M. Application of RAPD for molecular characterization of plant species of medicinal value from an arid environment. Genet Mol Res.2010;9:2191-2198. 9. Liu P, Yang YS, Hao CY Guo WD. Ecological risk assessment using RAPD and distribution pattern of a rare and endangered species. Chemosphere 2007;68:1497-1505. 10. Zheng W, Wang L, Meng L, Liu J. Gene variation in the endangered Anisodu Tanguticus (Solanacceae) an alphine perennial endemic to the Qinghai-Tibetan Plateau. Genetica 2008; 132:123-129. 11. Sifau MO, Akinpelu A, Ogunkanmi LA, Adekoya KO, Oboh BO, Ogundipe OT. Genetic diversity in Nigerian brinjal egg plant (Solanum melongena L.) as revealed by random amplified polymorphic DNA (RAPD) markers. Afr J Biotechnol2014;13:2119-2126. 12. Sharma A, Kaur R, Gauguly NK, Singh PD, Chakraborti A. Subtype distribution of Haemophilus influenzae isolates from north India. J Med Microbiol 2002;51:399-404. 13. Eboh DE. Fingerprint patterns in relation to gender and blood group among students of Delta State University, Abraka, Nigeria. J Exp Clin Anat 2013;12:82-86. 14. Oladapo OO, Igbigbi PS. Palmar and digital dermatogylyphic patterns of the three ethnic groups in Nigeria. Afr J Med Med Sci 2009;37:333-337. 15. Pavel AB, Vasile CL. PyElph - a software tool for gel images analysis and phylogenetics. BMC bioinformatics 2012;13:9. 16. Papadopoulos S, Benter T, Anastassiou G, Pape M, Gerhard S, Bornfeld N, Ludwig WD, Dorken B. Assessment of genomic instability in breast cancer and uveal melanoma by random amplified polymorphic DNA analysis. Int J Cancer 2002;99:193-200. 17. Grundman H, Scheider C, Hartung D, Daschner FD, Pitt TL. Discriminatory power of three based typing techniques or P. aeruginosa. J Clin Microbiol1995; 33:528-534. 18. Ismaeel HM. Identification of genomic markers by RAPD – PCR Primers in Iraq Breast Cancer patients. Iraq J Sci 2013;54:97-104. 19. Bryc K, Auton A, Nelson MR, Oksenberg JR, Hauser SL, Williams S, Froment A, Bodo JM,Wambebe C, Tishkoff SA, Bustamante CD. Genome-wide patterns of population structure and admixture in West Africans and African Americans. Proc Natl Acad Sci USA 2010; 107:786-791. | ||
|
آمار تعداد مشاهده مقاله: 1,843 تعداد دریافت فایل اصل مقاله: 2,169 |
||