| تعداد نشریات | 24 |
| تعداد شمارهها | 903 |
| تعداد مقالات | 8,081 |
| تعداد مشاهده مقاله | 15,203,535 |
| تعداد دریافت فایل اصل مقاله | 12,847,894 |
Evaluation of genetic relationship between 15 bamboo species of North-East India based on ISSR marker analysis | ||
| Molecular Biology Research Communications | ||
| مقاله 2، دوره 7، شماره 1، 2018، صفحه 7-15 اصل مقاله (286.42 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2018.28378.1303 | ||
| نویسندگان | ||
| Thoungamba Amom؛ Leimapokpam Tikendra؛ Hamidur Rahaman؛ Angamba Potshangbam؛ Potshangbam Nongdam* | ||
| Deparment of Biotechnology, Manipur University, India | ||
| چکیده | ||
| The classification of bamboos based on floral morphology and reproductive characters is very hard due to erratic flowering behavior and unusually long reproductive cycle. The application of reliable and effective DNA molecular markers is highly essential to address this problem. In the present investigation, inter-simple sequence repeats (ISSR) markers were employed to study phylogenetic relationship of 15 different bamboos of North-East India. The 10 ISSR primers generated 116 polymorphic loci or scorable bands with average of 11.6 bands per primer. The genetic similarity coefficient ranged from 0.41-0.76 showing high genetic polymorphism among different bamboo species. The phylogenetic tree constructed based on genetic similarity matrix revealed genetic proximity of endemic Bambusa mizorameana to other five Dendrocalamus species by clustering into a single group, while Dendrocalamus manipureanus segregated from the cluster indicating its genetic divergent character. Except for Schizotachyum fuchsianum, the three Schizotachyum species viz., S. dullooa, S. pergracile and S. munroi exhibited close genetic affinity by grouping into a single minor clade. Principal coordinates analysis (PCoA) showed distribution of different bamboos species in the plot in accordance to unweighted pair-group method with arithmetic average (UPGMA) cluster analysis. Genetic relationship of 15 different bamboos as revealed from the dendrogram and PCoA analysis reasonably conformed to traditional system of classification though slight disagreement existed. This is the first report on the successful use of ISSR markers in the phylogenetic and genetic variation studies of 15 important bamboos of the region including the endemic bamboo species of B. mizorameana, B. manipureana, D. sikkimensis and D. manipureanus. | ||
| کلیدواژهها | ||
| bamboos؛ Genetic diversity؛ ISSR markers؛ Bambusa؛ Dendrocalamus | ||
| مراجع | ||
|
1. Scurlock JMO, Dayton DC, Hames B. Bamboo: An overlooked biomass resource? Biomass Energy 2000;19:229-244.
2. Nongdam P, Tikendra L. The nutritional facts of bamboo shoots and their usage as important traditional foods of northeast India. Int Sch Res Notices 2014;2014:17.
3. Das M, Bhattacharya S, Singh P, Filgueiras T, Pal A. Bamboo taxonomy and diversity in the era of molecular markers. Adv Bot Res 2008;47:225-268.
4. Ohrnberger D, Goerrings J. The Bamboos of the World. 1986; Odenthal, Germani.
5. Ohrnberger D, Goerrings J. The Bamboos of the World. 2002; Elsevier Science, Amsterdam.
6. Brown-Guedira GL, Thompson JA, Nelson RL, Warburton ML. Evaluation of genetic diversity of soybean introductions and North American ancestors using RAPD and SSR markers. Crop Sci 2000;40:815-823.
7. Fan D, Jiarong X, Yuming Y, Chaomao H, Jing W. Study on flowering phenomenon and its type of bamboo in Yunnan in past fifteen years. Sci Silvae Sin 2000; 3: 57–68.
8. Ramanayake SMSD, Meemaduma VN, Weerawardene TE. Genetic diversity and relationships between nine species of bamboo in Sri Lanka, using random amplified polymorphic DNA. Plant Syst Evol 2007;269:55-61.
9. Nayak S, Rout GR, Das P. Evaluation of the genetic variability in bamboo using RAPD markers. Plant Soil Environ 2003;49:24-28.
10. Nongdam P, Nirmala C. Genetic variability in four species of Cymbidium based on isozyme markers. Physiol Mol Biol Plants2007;13:65-68.
11. Tiwari G, Singh R, Singh N, Choudhury DR Paliwal R, Kumar A, Gupta V. Study of arbitrarily amplified (RAPD and ISSR) and gene targeted (SCoT and CBDP) markers for genetic diversity and population structure in Kalmegh [Andrographis paniculata (Burm. f.) Nees]. Ind Crops Prod 2016; 86:1-11.
12. Uma MS, Hegde N, Hittalmani S. Identification of SSR marker associated with rust resistance in cowpea (Vigna unguiculata L.) using bulk segregant analysis. Legume Res 2016; 39:39-42.
13. Sebastiani MS, Ficcadenti N. In vitro plant regeneration from cotyledonary explants of Cucumis melo L.var. cantalupensis and genetic stability evaluation using RAPD analysis. Plant Cell Tissue Organ Cult2016;124:69-79.
14. Chu YE, Chou TS, Li YS, Shih CY, Woo SC. Identification of Bamboo clones, Dendrocalamus Latiflorus, in Taiwan. Bot Bull Acad Sin 1972;13:11-18.
15.Das M, Bhattacharya S, Basak J, Pal A. Phylogenetic relationships among the bamboo species as revealed by morphological characters and polymorphism analyses. Biol Plantarum 2007;51:667-672.
16. Tiwari C, Bakshi M. Isozymatic characterization of accessions of Arundinaria falcata (Nees). For Res 2014;4:133.
17. Friar E, Kochert G. A study of genetic variation and evolution of Phyllostachys (Bambusoideae: Poaceae) using nuclear restriction fragment length polymorphisms. Theor Appl Genet 1994;89:265-270.
18. Loh JP, Kiew R, Set O, Gan LH, Gan YY. A study of genetic variation and relationships within the bamboo subtribe Bambusinae using amplified fragment length polymorphism. Ann Bot 2000;85:607-612.
19. Wang MX, Zhang HL, Zhang DL, Qi YW, Fan ZL, Li DY, Pan DJ, Cao YS, Qiu ZE, Yu P, Yang QW, Wang XK, Li ZC. Genetic structure of Oryza rufipogon Griff. in China. Heredity 2008;101:527-535.
20. Razaq M, Heikrujam M, Chetri SK, Agrawal V. In vitro clonal propagation and genetic fidelity of the regenerants of Spilanthes calva DC. using RAPD and ISSR marker. Physiol Mol Biol Plants2013;19:251-260.
21. Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 1996;2:225-238.
22. Saha S, Adhikari S, Dey T, Ghosh P. RAPD and ISSR based evaluation of genetic stability of micropropagated plantlets of Morus alba L. variety S-1. Meta Gene 2016;7:7-15.
23. Yang HQ, An MY, Gu ZJ, Tian B. Genetic diversity and differentiation of Dendrocalamus membranaceus (Poaceae: Bambusoideae), a declining bamboo species in Yunnan, China, as based on Inter-Simple Sequence Repeat (ISSR) analysis. Int J Mol Sci 2012;13:4446-4447.
24. Tian B, Yang HQ, Wong KM, Liu AZ, Ruan ZY. ISSR analysis shows low genetic diversity versus high genetic differentiation for giant bamboo, Dendrocalamus giganteus (Poaceae: Bambusoideae), in China populations. Genet Resour Crop Ev 2012;59:901-908.
25. Nilkanta H, Amom T, Tikendra L, Rahaman H, Nongdam P. ISSR Marker Based Population Genetic Study of Melocanna baccifera (Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India. Scientifica 2017;2017:9.
26. Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochem Bull 1987;19:11-15.
27. Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 1979;76:5269-5273.
28. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-2739.
29. Peakall R, Smouse PE. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research- an update. Bioinformatics 2012;28:2537-2539.
30. Zhou ZY, Xia QY, Lu C, Feng LC, Xiang ZH 1998. The correlation between the number of RAPD-loci and the reliability of the information on genetic variation in molecular phylogenetic studies. Hereditas (Beijing) 1998;20:12-15.
31. Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 1978;89:583-590.
32. Ghosh S, Somkuwar B, Mandi SS, Talukdar NC. Genetic variability and phylogenetic relationship among some bamboo species of North-East India by AFLP analysis. Asian J Plant Sci Res 2012;2:478-485.
33. Naithani HB, Bisht NS, Singsit S. Distribution of Bamboo species in Manipur. 2010; Forest Department, Government of Manipur, India.
34. Basumatary A, Middha SK, Usha T, Bhattacharya S, Brahma BK, Goyal AK. Morphological phylogeny among 15 accessions of bamboos growing in Kokrajhar district of the Bodoland Territorial Area Districts, Assam. J For Res 2017; DOI 10.1007/s11676-017-0535-z.
35. Sun Y, Xia N, Stapleton CMA. Relationships between Bambusa species (Poaceae, Bambusoideae) revealed by random amplified polymorphic DNA. Biochem Syst Ecol 2006; 34: 417-423.
36. Desai P, Mankad M, Shah S, Patel A, Patil G, Narayanan S, Kumar N. Comparative assessment of genetic diversity among Indian bamboo genotypes using RAPD and ISSR markers. Mol Bio Rep 2015;42(8):1265-1273.
37. Goyal AK, Sen A. Phylogenetic relationships among accessions of bamboos encountered in North Bengal, India based on RAPD and ISSR markers. Indian J Biotechnol 2015;14:459-503. | ||
|
آمار تعداد مشاهده مقاله: 2,924 تعداد دریافت فایل اصل مقاله: 3,394 |
||