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Comparison of virulence genes in Proteus species isolated from human and pet turtle | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 10، دوره 19، شماره 1 - شماره پیاپی 62، 2018، صفحه 48-52 اصل مقاله (247.07 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2018.4768 | ||
| نویسندگان | ||
| H. N. K. S. Pathirana1؛ B. C. J. De Silva1؛ S. H. M. P. Wimalasena1؛ S. Hossain2؛ G. J. Heo* 3 | ||
| 1MSc Student in Veterinary Medicine, Department of Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Korea | ||
| 2Ph.D. Student in Veterinary Medicine, Department of Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Korea | ||
| 3Laboratory of Aquatic Animal Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Korea | ||
| چکیده | ||
| The current study was aimed to investigate the prevalence of ureC, rsbA, zapA and mrpA virulence genes using polymerase chain reaction (PCR) in Proteus spp. isolated from 5 commercially popular species of pet turtles and comparison of the mrpA gene sequences of Proteus mirabilis isolates with human clinical isolates. A total of 24 isolates in pet turtles were identified, comprised of P. mirabilis (15), Proteus vulgaris (7) and Proteus hauseri (2). The prevalence of ureC, rsbA, zapA and mrpA genes among all identified Proteus spp. isolates were 91.7%, 50%, 45.8% and 45.8%, respectively. The average percentage similarities of mrpA gene sequence of pet turtle P. mirabilis isolates to human urinary and respiratory isolates were 96.35% and 94.85%, respectively. The prevalence of virulence genes and high similarity of mrpA gene sequences between pet turtles and human P. mirabilis isolates revealed that though pet turtles are healthy, these animals may pose a potential risk of urinary and respiratory infections to humans. | ||
| کلیدواژه‌ها | ||
| mrpA gene؛ Pet turtles؛ Proteus spp؛ Virulence genes | ||
| مراجع | ||
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Abbas, KF; Jawad, K; Khafaji, AL; Maysaa, S and Shukri, AL (2015). Molecular detection of some virulence genes in Proteus mirabilis isolated from Hillaprovince. Int. J. Res. Stud. in Biosci. (IJRSB)., 3: 85-89.
Ali, HH and Yousif, GM (2015). Detection of some virulence factors genes of Proteus mirablis that isolated from urinary tract infection. Int. J. of Adv. Res., 3: 156-163.
Back, DS; Shin, GW; Wendt, M and Heo, GJ (2016). Prevalence of Salmonella spp. in pet turtles and their environment. Lab. Anim. Res., 32: 166-170.
Barbour, EK; Hajj, ZG; Hamadeh, S; Shaib, HA; Farran, MT; Araj, G; Faroon, O; Barbour, KE; Jirjis, F; Azhar, E; Kumosani, T and Harahesh, S (2012). Comparison of phenotypic and virulence genescharacteristics in human and chicken isolates of Proteus mirabilis. Path. Glob. Health. 106: 352-357.
Bluvias, JE and Eckert, KL (2010). Marine turtle trauma response procedures: a husbandry manual. Wider Caribbean Sea Turtle Conservation Network (WIDER) Technical Report No. 10, Ballwin, Missouri. P: 100.
Dalia, AA (2015). Prevalence of Proteus spp. in some hospitals in Baghdad city. Iraqi. J. Sci., 56: 665-672.
Drzewiecka, D (2016). Significance and roles of Proteus spp. bacteria in natural environments. Microb. Ecol., 72: 741-758.
Harada, K; Niina, A; Shimizu, T; Mukai, Y; Kuwajima, K; Miyamoto, T and Kataoka, Y (2014). Phenotypic and molecular characterization of antimicrobial resistance in Proteus mirabilis isolates from dogs. J. Med. Microbiol., 63: 1561-1567.
Hegazy, WAH (2016). Diclofenac inhibits virulence of Proteus mirabilis isolated from diabetic foot ulcer. Afr. J. of Microbiol. Res., 10: 733-743.
Henriksen, P (1972). Diagnosis and treatment of disease in the turtle. Iowa State Univ. Vet., 34: 8.
Hidalgo-Vila, J; Diaz-Paniagua, C; De Frutos-Escobar, C; Jimenez-Martinez, C and Perez-Santigosa, N (2007). Salmonella in free living terrestrial and aquatic turtles. Vet. Microbiol., 119: 311-315.
Hordijk, J; Schoormans, A; Kwakernaak, M; Duim, B; Broens, E; Dierikx, C; Mevius, D and Wagenaar, JA (2013). High prevalence of fecal carriage of extended spectrum beta-lactamase/AmpC-producing Entero-bacteriaceae in cats and dogs. Front. Microbiol., 4: 242.
Hossain, S; Wimalasena, SHMP and Heo, GJ (2016). Virulence factors and antimicrobial resistance pattern of Citrobacter freundii isolated from healthy pet turtles and their environment. Asi. J. Ani. Vet. Adv., 12: 10-16.
Li, X and Mobley, HL (2002). Vaccines for Proteus mirabilis in urinary tract infection. Int. J. Antimicrob. Agents. 19: 461-465.
Manos, J and Belas, R (2006). The genera Proteus, Providencia, and Morganella. Prokaryotes. 6: 245-269.
Mermin, J; Hutwagner, L; Vugia, D; Shallow, S; Daily, P; Bende, J; Koehler, J; Marcus, R and Angulo, FJ (2004). Reptiles, amphibians, and human Salmonella infection: a population-based, case-control study. Clin. Inf. Dis., 38: 253-261.
Mohammed, SO; Elshahaby, OA; Hafez, EE; Mohammed, AK and Ahmed, E (2014). Characterization and purification of urease enzyme from new Proteus mirabilis strain. J. Adv. Sci. Res., 5: 8-11.
Omoruyia, EA and Evangelista, M (2014). Proteus mirabilis septicemia and meningitis in a neonate. J. Med. Cases. 5: 245-247.
Pearson, MM; Rasko, DA; Smith, SN and Mobley, HL (2010). Transcriptome of swarming Proteus mirabilis. Infect. Immun., 78: 2834-2845.
Rather, PN (2005). Swarmer cell differentiation in Proteus mirabilis. Environ. Microbiol., 7: 1065-1073.
Rocha, SP; Elias, WP; Cianciarullo, AM; Menezes, MA; Nara, JM; Piazza, RM; Silva, MR; Moreira, CG and Pelayo, JS (2007). Aggregative adherence of uropathogenic Proteus mirabilis to cultured epithelial cells. FEMS Immunol. Med. Microbiol., 51: 319-326. Różalski, A; Torzewska, A; Moryl, M; Kwil, I; Maszewska, A; Ostrowska, K; Drzewiecka, D; Zabłotni, A; Palusiak, A; Siwinska, M and Staçzek, P (2012). Proteus spp. an opportunistic bacterial pathogen-classification, swarming growth, clinical significance and virulence factors. Folia Biol. Oeco., 8: 1-17.
Schaffer, JN and Pearson, MM (2015). Proteus mirabilis and urinary tract infections. Microbiol. Spectr., 3(5): UTI-0017-2013. doi: 10.1128/microbiolspec.UTI-0017-2013.
Senior, BW (1997). Media and tests to simplify the recognition and identification of members of the Proteeae. J. Med. Microbiol., 46: 39-44.
Trivedi, KM; Branton, A; Trivedi, D; Nayak, G; Mondal, SC and Jana, S (2015). Phenotyping and genotyping characterization of Proteus vulgaris; after biofield treatment. Int. J. Genetics and Genomics., 3: 66-73.
Walker, KE; Moghaddame-Jafari, S; Lockatell, CV; Johnson, D and Belas, R (1999). ZapA, the IgA-degrading metalloprotease of Proteus mirabilis, is a virulence factor expressed specically in swarmer cells. Mol. Microbiol., 32: 825-836. | ||
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