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Study of TALP and TRIS citrate medium on caprine sperm capacitation and subsequent in vitro embryo production | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 8، دوره 18، شماره 4 - شماره پیاپی 61، 2017، صفحه 275-278 اصل مقاله (162.27 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2017.4633 | ||
| نویسندگان | ||
| S. Agarwal1؛ S. D. Kharche* 2؛ A. K. Bhatiya3 | ||
| 1Ph.D. Scholar in Biotechnology, Department of Biotechnology, Institute of Applied Science and Humanities, GLA University, Mathura, Uttar Pradesh, India | ||
| 2Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India | ||
| 3Department of Biotechnology, Institute of Applied Science and Humanities, GLA University, Mathura, Uttar Pradesh, India | ||
| چکیده | ||
| The aim of the present investigation was to compare Tyrode’s albumin lactate pyruvate (TALP) medium and TRIS citrate medium for capacitation of caprine sperm. In experiment 1 capacitation was assessed by chlortetracycline assay and in experiment 2 with in vitro fertilization and embryo development. In experiment 2, cumulus oocyte complexes (COCs) recovered by slicing the caprine ovaries were matured in maturation medium for 27 h in humidified atmosphere at 38.5°C with 5% CO2. After 27 h of culture a total of 2480 in vitro matured oocytes were selected and randomly divided into two groups. Group 1 (n=1124) matured oocytes were fertilized by the spermatozoa capacitated in TALP medium and in group 2 (n=1356) matured oocytes were fertilized by the spermatozoa capacitated in TRIS citrate medium. The results of experiment 1 indicated a comparatively more number of sperms with Chlortetracycline (CTC) Pattern B in TRIS citrate than TALP medium (55.32 ± 0.91% vs 47.96 ± 0.20%). In experiment 2, the cleavage rate and blastocyst production were higher following capacitation of spermatozoa in TRIS citrate than TALP medium. In conclusion, TRIS citrate can be used as an alternative and effective media for sperm capacitation to get higher cleavage rate and blastocyst production in goat. | ||
| کلیدواژهها | ||
| Blastocyst؛ Capacitation؛ Caprine spermatozoa؛ TRIS citrate medium | ||
| مراجع | ||
|
Aitken, RJ and Nixon, B (2013). Sperm capacitation: a distant landscape glimpsed but unexplored. Mol. Hum. Reprod., 19: 785-793.
Bavister, BD (2002). Early history of in vitro fertilization. Reproduction. 124: 181-196.
Bedford, JM (1983). Significance for need for sperm capacitation before fertilization in eutherian mammals. Biol. Reprod., 28: 108-120.
Braude, P; Bolton, V and Moore, S (1988). Human gene expression first occurs between the four- and eight-cell stages of preimplantation development. Nature. 332: 459-461.
Crozet, N; Ahmed-Ali, M and Dubos, MP (1995). Developmental competence of caprine oocytes from follicles of different size categories following maturation, fertilization and culture in vitro. J. Reprod. Fertil., 103: 293-298.
Dar, S; Grover, SA; Moskovtsev, SI; Swanson, S; Baratz, A and Librach, CL (2013). In vitro fertilization-intracytoplasmic sperm injection outcome in patients with a markedly high DNA fragmentation index (>50%). Fertil. Steril., 100: 75-80.
Elkhawagah, AR; Longobardi, V; Gasparrini, B; Sosa, GA; Bifulco, G; Abouelroos, MEA; Abd el-ghafar, AE and Campanile, G (2013). Evaluation of in vitro capacitation of buffalo frozen/thawed sperm by different tsechniques. J. Reprod. Infertil., 4: 19-28.
First, NL and Parrish, JJ (1987). In-vitro fertilization of ruminants. J. Reprod. Fertil., 34: 151-165.
Fraser, LR; Abeydeera, LR and Niwa, K (1995). Ca(2+)-regulating mechanisms that modulate bull sperm capacitation and acrosomaleocytosis as determined by chlortetracycline analysis. Mol. Reprod. Dev., 40: 233-241.
Gangwar, C; Kharche, SD; Ranjan, R; Kumar, S; Goel, AK; Jindal, SK and Agarwal, SK (2015). Effect of vitamin C supplementation on freezability of Barbari buck semen. Small Rum. Res., 22: 104-107.
Kharche, SD; Agarwal, S; Sharma, J; Sikarwar, AKS; Gangwar, C; Ranjan, R; Goel, AK; Jindal, SK and Agarwal, SK (2016). Influence of cysteamine supplementation during in vitro culture of early stage caprine embryos on blastocyst production. Indian J. Anim. Sci., 86: 304-306.
Kharche, SD; Goel, AK; Jindal, SK; Goel, P and Jha, BK (2011). Birth of twin kids following transfer of in-vitro produced caprine embryos. Indian J. Anim. Sci., 81: 1132-1134.
Kharche, SD; Goel, AK; Jindal, SK and Sinha, NK (2007). Birth of a female kid from in-vitro matured and fertilized caprine oocytes. Indian J. Anim. Sci., 78: 680-685.
Kharche, SD; Goel, AK; Jindal, SK; Sinha, NK and Yadav, P (2008a). Effect of somatic cells co-cultures on cleavage and development of in-vitro fertilized caprine embryos. Indian J. Anim. Sci., 78: 686-692.
Kharche, SD; Jindal, SK; Priyadhrashini, R; Kumar, S; Goel, AK; Ramachandran, N and Rout, PK (2013). Fertility following frozen semen artificial insemination in Jamunapari goats. Indian J. Anim. Sci., 83: 1071-1073.
Kharche, SD; Yadav, EN; Goel, AK; Jindal, SK and Sinha, NK (2008b). Influence of culture media on in-vitro fertilization of caprine oocytes. Indian J. Anim. Sci., 78: 1075-1077. Perez, LJ; Vakkrcel, A; de lasHeras, MA; Moses, DF and Baldassarre, H (1996). In vitro capacitation and induction of acrosomal exocytosis in ram spermatozoa as assessed by the chlortetracycline assay. Theriogenology. 45: 1037-1046.
Slavik, T and Fulka, J (1992). In vitro fertilization of intact sheep and cattle oocytes with caprine spermatozoa. Theriogenology. 38: 721-726.
Snedecor, GW and Cochran, WG (1989). Statistical methods. 8th Edn., Ames, IowaStateUniversity Press. P: 466.
Wu, B; Zan, L; Quan, F and Wang, H (2015). A novel discipline in embryology — animal embryo breeding. In: Wu, B (Ed.), New discoveries in embryology. (1st Edn.), Published by AvE4EvA. PP: 185-198.
Yanagimachi, R (1994). Fertility of mammalian spermatozoa: its development and relativity. Zygote. 2: 371-372. | ||
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