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Effects of different activation protocols on cleavage rate and blastocyst production of caprine oocytes | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 2، دوره 18، شماره 4 - شماره پیاپی 61، 2017، صفحه 243-248 اصل مقاله (227.38 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2017.4618 | ||
| نویسندگان | ||
| J. Pathak1؛ S. D. Kharche* 2؛ A. Goel3 | ||
| 1Ph.D. Scholar in Biotechnology, Department of Biotechnology, Institute of Applied Science and Humanities, GLA University, Mathura, Uttar Pradesh, India | ||
| 2Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India | ||
| 3Department of Biotechnology, Institute of Applied Science and Humanities, GLA University, Mathura, Uttar Pradesh, India | ||
| چکیده | ||
| The present study was undertaken to assess the effect of different chemical activators along with 6-DMAP on in vitro matured caprine oocytes. From 4332 ovaries, 14235 cumulus oocyte complexes (COCs) were collected which were matured in TCM-199 medium containing follicle stimulating hormone (FSH) (5 µg/ml), Leutinizing hormone (LH) (10 µg/ml), oestradiol-17β (1 µg/ml) supplemented with 10% fetal bovine serum, 10% follicular fluid and 3 mg/ml bovine serum albumin (BSA) at 38.5°C and 5% CO2 in an incubator under humidified air for 27 h. In group 1 (control), 3117 in vitro matured oocytes were co incubated with sperms for 18 h in ferttalp medium. In group 2, 3563 in vitro matured oocytes were activated with 7% ethanol for 5-7 min followed by treatment with 2.0 mM DMAP for 4 h in mCR2aa medium. In group 3, 3109 in vitro matured oocytes were activated with 5 μM ionomycin for 5-7 min followed by treatment with 2.0 mM DMAP for 4 h in mCR2aa medium. In group 4, 3455 in vitro matured oocytes were activated with 5 μM calcium ionophore for 5-7 min followed by treatment with 2.0 mM DMAP for 4 h in mCR2aa medium. Oocytes were cultured in 50 µL drops of research vitro cleave (RVCL) medium for embryo development. The cleavage rate, morula and blastocyst production in group 1, 2, 3 and 4 were 26.07 ± 2.37%, 14.91 ± 2.91 & 1.45 ± 0.71%, 49.57 ± 3.79%, 20.07 ± 2.38% & 5.29 ± 1.42%, 50.18 ± 3.59%, 15.26 ± 2.87% & 1.85 ± 0.72% and 80.26 ± 2.30%, 35.33 ± 2.67 & 7.10 ± 0.89%, respectively. These results indicated that the activation of in vitro matured oocytes by 5 μM calcium ionophore for 5-7 min followed by treatment with 2.0 mM DMAP for 4 h is most favorable for parthenogenetic caprine embryos production. | ||
| کلیدواژهها | ||
| Blastocyst؛ Calcium ionophore؛ Caprine؛ Cleavage؛ Parthenogenesis | ||
| مراجع | ||
|
Abdoon, AS; Ghanem, N; Kandila, OM; Gad, A; Schellander, K and Tesfaye, D (2012). cDNA microarray analysis of gene expression in parthenotes and in vitro produced buffalo embryos. Theriogenology. 77: 1240-1251.
Abdullah, RB; Wan Khadijah, WE and Kwong, PJ (2011). Comparison of intra- and interspecies nuclear transfer techniques in the production of cloned caprine embryos. Small Rumin. Res., 98: 196-200.
De, AK; Garg, S; Singhala, DK; Malika, H; Mukherjee, A; Jena, MK; Kumar, S; Kaushik, JK; Mohanty, AK; Das, BC; Bag, S; Bhanja, SK and Malakar, D (2013). Derivation of goat embryonic stem cell-like cell lines from in vitro produced parthenogenetic blastocysts. Small Rumin. Res., 113: 145-153.
De, LFR and King, WA (1998). Developmental consequences of karyokinesis without cytokinesis during the first mitotic cell cycle of bovine parthenotes. Biol. Reprod., 58: 952-962.
De, AK; Malakar, D; Jena, MK; Dutta, R; Garg, S and Akshey, YS (2012). Zona-free and with-zonaparthenogenetic embryo production in goat (Capra hircus) - effect of activation methods, culture systems and culture media. Livest. Sci., 143: 35-42.
Dyban, AP and Baranow, WS (1989). Haploidie. In: Dyban, AP and Baranow, WS (Eds.), The cytogenetics of the sucker – embryogenesis. (1st Edn.), Pareys Studientexte64, Berlin und Hamburg, Verlag Paul Parey. PP: 1-43.
Funahashi, H; Cantley, T; Stumpf, TT; Terlouw, SL; Rieke, A and Day, BN (1994). In vitro development of in vitro matured pig oocytes following chemical activation or in vitro fertilization. Biol. Reprod., 50: 1072-1077.
Hosseini, SM; Hajian, M; Moulavi, F; Shahverdi, AH and Nasr-Esfahani, MH (2008). Optimized combined electrical-chemical parthenogenetic activation for in vitro matured bovine oocytes. Anim. Reprod. Sci., 108: 122-133.
Jena, MK; Malakar, D; De, AK; Garg, S; Akshey, YS; Dutta, R; Sahu, S; Mohanty, AK and Kaushik, JK (2012). Handmade cloned and parthenogenetic goat embryos – A comparison of different culture media and donor cells. Small Rumin. Res., 105: 255-262.
Kharche, SD and Birade, HS (2013). Parthenogenesis and activation of mammalian oocytes for in vitro embryo production: a review. Adv. Bio. Sci. Biotechnol., 4: 170-182.
Kharche, SD; Goel, AK; Jindal, SK; Goel, P and Jha, BK (2011). Birth of twin kids following transfer of in-vitro produced caprine embryos. Indian J. Anim. Sci., 81: 1132-1134.
Kharche, SD; Goel, AK; Jindal, SK; Jha, BK and Goel, P (2013). Assessment of parthenogenetic embryo production by activation of in-vitro matured caprine oocytes with different concentrations of ethanol. Small Rumin. Res., 111: 100-103.
Kharche, SD; Goel, AK; Jindal, SK; Sinha, NK and Yadav, P (2008). Effect of somatic cells co-cultures on cleavage and development of in vitro fertilized caprine embryos. Indian J. Anim. Sci., 78: 686-692.
Kharche, SD; Goel, AK; Jindal, SK; Ranjan, R; Rout, PK; Agarwal, SK; Goel, P; Saraswat, S; Vijh, RK; Malakar, D; Bag, S; Sarkhel, B and Bhanja, SK (2014). Development of parthenote following in vivo transfer of embryos in Capra hircus. In Vitro Cell. Dev. Biol., 50: 893-898.
Kharche, S; Pathak, J; Agarwal, S; Kushwah, B and Sikarwar, AKS (2016). Effect of Ca ionophore on blastocyst production following intracytoplasmic sperm injection in caprine oocytes. Reprod. Dom. Anim., 51: 611-617.
Kim, NH; Simerly, C; Funahashi, H; Schatten, G and Day, BN (1996). Microtubule organization in porcine oocytes during fertilization and parthenogenesis. Biol. Reprod., 54: 1397-1404.
King, WA; Xu, KP; Sirard, MA; Greve, T; Leclerc, P; Lambert, RD and Jacques, P (1988). Cytogenetic study of parthenogenetically activated bovine oocytes matured in vitro and in vitro. Gamete Res., 20: 265-274.
Kono, T; Iwasaki, S and Nakahara, T (1989). Parthenogenetic activation by electric impulse of bovine oocytes matured in vitro. Theriogenology. 32: 569-576.
Kouamo, J and Kharche, SD (2015). A comparative study of parthenogenetic activation and in vitro fertilization of in vitro matured caprine oocytes. Iran. J. Vet. Res., 16: 20-24.
Lee, SR; Kim, JW; Kim, BS; Kim, MO; Kim, SH; Yoo, DH; Shin, MJ; Lee, S; Park, YS; Park, YB; Ha, JH and Ryoo, ZY (2007). The parthenogenetic activation of canine oocytes with Ca-EDTA by various culture periods and concentrations. Theriogenology. 67: 698-703.
Liu, L and Yang, X (1999). Interplay of maturation-promoting factor and mitogen-activated protein kinase inactivation during metaphase-to-interphase transition of activated bovine oocytes. Biol. Reprod., 61: 1-7.
Loi, P; Ledda, S; Fulka, JJ; Cappai, P and Moor, RM (1998). Development of parthenogenetic and cloned ovine embryos; effect of activation protocols. Biol. Reprod., 58: 1177-1187.
Machaty, Z; Funahashi, H; Mayes, MA; Day, B and Prather, RS (1996). Effects of injecting calcium chloride into in vitro matured porcine oocytes. Biol. Reprod., 54: 316-322.
Max, A; Grabiec, A and Tischner, M (2007). Partheno-genetic activation of domestic cat oocytes using ethanol, calcium ionophore, cycloheximide and a magnetic field. Theriogenology. 67: 795-800.
Mayes, MA; Stogsdill, PL and Prather, RS (1995). Partheno-genic activation of pig oocytes by protein kinase inhibition. Biol. Reprod., 53: 270-275.
Meo, SC; Leal, CLV and Garcia, JM (2004). Activation and early parthenogenesis of bovine oocytes treated with ethanol and strontium. Anim. Reprod. Sci., 81: 35-46.
Nussbaum, DJ and Prather, RS (1995). Differential effects of protein synthesis inhibitors on porcine oocyte activation. Mol. Reprod. Dev., 195: 70-75.
Ongeri, EM; Bormann, CL; Butler, RE; Melican, D; Gavin, WG; Echelard, Y; Krisher, RL and Behboodi, E (2001). Development of goat embryos after in vitro fertilization and parthenogenetic activation by different methods. Theriogenology. 55: 1933-1945.
Pathak, J; Kharche, SD; Goel, AK and Jindal, SK (2013). A comparative study on parthenogenetic activation and embryo production from in vitro matured caprine oocytes. Small Rumin. Res., 113: 136-140.
Presicce, GA and Yang, X (1994). Nuclear dynamics of parthenogenesis of bovine oocytes matured in vitro for 20 and 40 h and activated with combined ethanol and cycloheximide. Mol. Reprod. Dev., 37: 61-68.
Sato, K; Yoshida, M and Miyoshi, K (2005). Utility of ultrasound stimulation for activation of pig oocytes matured in vitro. Mol. Reprod. Dev., 72: 396-403.
Sharma, JR; Agarwal, S; Kharche, SD; Goel, AK; Jindal, SK and Agarwal, SK (2015). Effect of different activators on development of activated in vitro matured caprine oocytes. Iran. J. Vet. Res., 16: 42-46. Shiina, Y; Kaneda, M; Matsuyama, K; Tanaka, K; Hiroi, M and Doi, K (1993). Role of extracellular Ca2+ on the intracellular Ca2+ changes in fertilized and activated mouse oocytes. J. Reprod. Fertil., 97: 143-150.
Singh, R; Kumar, K; Mahapatra, PS; Kumar, M; Agarwal, P; Bhure, SK; Malakar, D; Bhanja, SK and Bag, S (2014). Microarray analysis of gene expression in parthenotes and in vitro-derived goat embryos. Theriogenology. 81: 854-860.
Soloy, E; Kanka, J; Viuff, D; Smith, SD; Callesen, H and Greve, T (1997). Time course of pronuclear deoxyribo-nucleic acid synthesis in parthenogenetically activated bovine oocytes. Biol. Reprod., 57: 27-35.
Srirattana, K; Sripunya, N; Sangmalee, A; Imsoonthornruksa, S; Liang, Y; Ketudat-Cairns, M and Parnpai, R (2013). Developmental potential of vitrified goat oocytes following somatic cell nuclear transfer and parthenogenetic activation. Small Rumin. Res., 112: 141-146.
Wang, Z; Wang, W; Yu, S and Xu, ZR (2008). Effects of different activation protocols on preimplantation development, apoptosis and ploidy of bovine parthenogenetic embryos. Anim. Reprod. Sci., 105: 292-301.
Whitaker, M and Patel, R (1990). Calcium and cell cycle control. Development. 108: 525-542.
White, KL and Yue, C (1996). Intracellular receptors and agents that induce activation in bovine oocytes. Theriogenology. 45: 91-100. | ||
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