| تعداد نشریات | 24 |
| تعداد شمارهها | 859 |
| تعداد مقالات | 7,646 |
| تعداد مشاهده مقاله | 13,577,938 |
| تعداد دریافت فایل اصل مقاله | 11,684,538 |
Biotic elicitation for scopolamine production by hairy root cultures of Datura metel | ||
| Molecular Biology Research Communications | ||
| مقاله 3، دوره 6، شماره 4، 2017، صفحه 169-176 اصل مقاله (309.34 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2017.25776.1275 | ||
| نویسندگان | ||
| Zahra Shakeran1؛ Mehrnaz Keyhanfar* 1؛ Mustafa Ghanadian2، 3 | ||
| 1Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, 81746-73441, Isfahan, Iran | ||
| 2Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 3Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| چکیده | ||
| The (-)-hyoscyamine, atropine and scopolamine (hyoscine) are three valuable tropane alkaloids while scopolamine is the most important member of this group for the pharmaceutical industry due to its higher demand compared to hyoscyamine and atropine. Scopolamine is an anticholinergic reagent with several therapeutic applications. In the current study, the hairy roots culture of Daturametel was used as an advantageous method for production of scopolamine. The hairy roots are formed by Agrobacterium rhizogenes and have genetic stability, high growth rate and lateral branching. In this study, the effect of Bacillus cereus and Staphylococcus aureus as biotic elicitors on the production of scopolamine in D. metel hairy roots was investigated. The amount of scopolamine in the hairy roots was detected by HPLC analysis and compared with control samples after 0, 12 and 24 hours. Results showed that, B. cereus and S. aureus enhanced scopolamine production in the culture while the atropine content was decreased. Although in the control samples with no bacterial elicitation no scopolamine was detected, elicitation by B. cereus caused production of scopolamine and about 0.03 gram and 0.017 gram of it was detected in 100 gram dried D. metel hairy roots after 12 and 24 hours respectively. In S. aureus elicited hairy roots, scopolamine was not produced after 12 hours. However, about 0.025 gram of this tropane alkaloid was detected in 100 gram dried hairy roots after 24 hours. In conclusion, S. areus and B. cereus induced the scopolamine production in D. metel hairy roots. | ||
| کلیدواژهها | ||
| Secondary metabolites؛ Bacillus cereus؛ Staphylococcus aureus | ||
| مراجع | ||
|
1. Canter PH, Thomas H, Ernst E. Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends Bioethanol 2005;23:180-185. 2. Marchev A, Georgiev V, Ivanov I, Pavlov A. Cultivation of diploid and tetraploid hairy roots of Datura stramonium L. in stirred tank bioreactor for tropane alkaloids production. J Biosci Bioethanol 2012;1:211-216. 3. Palazón J, Navarro-Ocaña A, Hernandez-Vazquez L, Mirjalili MH. Application of metabolic engineering to the production of scopolamine. Molecules 2008;13:1722-1742. 4. Ajungla L, Patil P, Barmukh R, Nikam T. Influence of biotic and abiotic elicitors on accumulation of hyoscyamine and scopolamine in root cultures of Datura metel L. Indian J Biotechnol 2009;8:317-322. 5. Jouhikainen K, Lindgren L, Jokelainen T, Hiltunen R, Teeri TH, Oksman-Caldentey K-M. Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering. Planta 1999;208:545-551. 6. Guillon S, Trémouillaux-Guiller J, Pati PK, Rideau M, Gantet P. Hairy root research: recent scenario and exciting prospects. Curr Opin Plant Biol 2006;9:341-346. 7. Matsumoto T, Nobukazu T. Production of phytoecdysteroids by hairy root cultures of Ajuga reptans var. atropurpurea. Agr Biol Chem Tokyo 1991;55:1019-1025. 8. Banerjee S, Singh S, Rahman LU. Biotransformation studies using hairy root cultures - a review. Biotechnol Adv 2012;30:461-468. 9. Shakeran Z, Keyhanfar M, Asghari G. Hairy roots formation in four Solanaceae species by different strains of Agrobacterium rhizogenes. J Med Plant By-Prod. 2014;3:155-160. 10. Chun J-A, Lee W-H, Han M-O, Lee J-W, Yi Y-B, Park G-Y, Chung C-H. Optimization of abiotic factors for improved growth and extracellular production of recombinant fungal phytase in sesame hairy root cultures. Biotechnol Bioprocess Eng 2007;12:242-249. 11. Kai G, Yang S, Zhang Y, Luo X, Fu X, Zhang A, Xiao J. Effects of different elicitors on yield of tropane alkaloids in hairy roots of Anisodus acutangulus. Mol Biol Rep 2012;39:1721-1729. 12. Jung H-Y, Kang S-M, Kang Y-M, Kang M-J, Yun D-J, Bahk J-D, Yang J-K, Choi M-S. Enhanced production of scopolamine by bacterial elicitors in adventitious hairy root cultures of Scopolia parviflora. Enzyme Microb Tech 2003;33:987-990. 13. Shakeran Z, Keyhanfar M, Asghari G, Ghanadian M. Improvement of atropine production by different biotic and abiotic elicitors in hairy root cultures of Datura metel. Turk J Biol 2015;39:111-118. 14. Rathbone DA, Bruce NC. Microbial transformation of alkaloids. Curr Opin Microbiol 2002;5:274-281. 15. Eskandari-Samet A, Piri K, Kayhanfar M, Hasanloo T. Enhancement of tropane alkaloid production among several clones and explants types of hairy root of Atropa belladonna L. J Med Plant By-Prod 2012;1:35-42. 16. Doyle JJ. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 1987;19:11-5. 17. Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual: Cold Spring harbor laboratory Cold Spring Harbor, NY; 1982. 18. Rahnama H, Hasanloo T, Shams MR, Sepehrifar R. Silymarin production by hairy root culture of Silybum marianum (L.) Gaertn. Iran J Biotechnol 2008;6:113-118. 19. Spollansky TC, Pitta-Alvarez SI, Giulietti AM. Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candida. Electron J Biotechnol 2000;3:31-32. 20. Bulgakov VP. Functions of rol genes in plant secondary metabolism. Biotechnol Adv 2008;26:318-324. 21. Kang Y, Park D, Min J, Song H, Jeong M, Kim Y, Kang S, Karigar C, Choi M. Enhanced production of tropane alkaloids in transgenic Scopolia parviflora hairy root cultures over-expressing putrescine N-methyl transferase (PMT) and hyoscyamine-6β-hydroxylase (H6H). In Vitro Cell Dev-Pl 2011;47:516-524. 22. Wu J-Y, Ng J, Shi M, Wu S-J. Enhanced secondary metabolite (tanshinone) production of Salvia miltiorrhiza hairy roots in a novel root–bacteria coculture process. Appl Microbiol Biot 2007;77:543-550. 23. Aziz ZFA, Saud HM, Rahim KA, Ahmed OH. Variable responses on early development of shallot (Allium ascalonicum) and mustard (Brassica juncea) plants to Bacillus cereus inoculation. Malayas J Microbiol 2012;8:47-50. 24. Zhang L, Ding R, Chai Y, Bonfill M, Moyano E, Oksman-Caldentey K-M, Xu T, Pi Y, Wang Z, Zhang H, Kai G. Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures. Proc Natl Acad Sci USA 2004;101:6786-6791. 25. Richter U, Rothe G, Fabian A-K, Rahfeld B, Dräger B. Overexpression of tropinone reductases alters alkaloid composition in Atropa belladonna root cultures. J Exp Bot 2005;56:645-652. 26. Zhang S, Liu Y, Shen S, Liang Z, Yang D. [Effects of elicitors on accumulation of phenolic acids and tanshinones in Salvia miltiorrhiza hairy root]. Zhongguo Zhong Yao Za Zhi 2011;36:1269-1274. [in Chinese] | ||
|
آمار تعداد مشاهده مقاله: 2,256 تعداد دریافت فایل اصل مقاله: 3,678 |
||