| تعداد نشریات | 24 |
| تعداد شمارهها | 908 |
| تعداد مقالات | 8,110 |
| تعداد مشاهده مقاله | 15,519,499 |
| تعداد دریافت فایل اصل مقاله | 13,166,484 |
In silico design and expression of a novel fusion protein of HBHA and high antigenic region of FAP-P of Mycobacterium avium subsp. paratuberculosis in Pichia pastoris | ||
| Molecular Biology Research Communications | ||
| مقاله 2، دوره 6، شماره 4، 2017، صفحه 161-168 اصل مقاله (179.9 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2017.26522.1286 | ||
| نویسندگان | ||
| Vida Eraghi؛ Abdollah Derakhshandeh* ؛ Arsalan Hosseini؛ Azar Motamedi-Boroojeni | ||
| Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| چکیده | ||
| Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne's disease in ruminants and there has been a shift in the public health approach to MAP and human diseases like Crohn's disease. The prevention of infection by MAP in ruminants is thought to deter the high impact of economic losses in the level of dairy industry and possible spreading of this pathogen in dairy products. The present study was done to investigate the construction and expression of the soluble form of a novel fusion protein, consisting of Heparin-binding hemagglutinin (HBHA) and high antigenic region of Fibronectin Attachment Protein-P (FAP-P), in order to introduce as a Th1 inducer subunit vaccine against MAP. HBHA is a mycobacterial adhesin and it has been demonstrated that a HBHA-specific IFN-γ response, in latent M. tuberculosis infection, depends on the methylation of the antigen. Further, FAP-P induces Th1 polarization. Because methylation of HBHA was not performed in E. coli, Pichia pastoris was chosen as the host. The desired fusion protein had a similar 3D structure to that of HBHA with its native form and post-translational methylation in C-terminal. Hence, the uptake of the purified fusion protein will be done by M cells because ofHBHA, and cell-mediated immunity will be induced because of both antigens. Eventually, successful construction and expression of the newly-designed chimeric protein under the mentioned conditions is reported in this article. | ||
| کلیدواژهها | ||
| FAP-P؛ fusion protein؛ HBHA؛ In silico؛ Mycobacterium avium subsp | ||
| مراجع | ||
|
1. Bannantine JP, Huntley JF, Miltner E, Stabel JR, Bermudez LE. The Mycobacterium avium subsp. paratuberculosis 35 kDa protein plays a role in invasion of bovine epithelial cells. Microbiology 2003;149:2061-2069. 2. Gurung RB, Purdie AC, Begg DJ, Whittington RJ. In silico screened Mycobacterium avium subsp. paratuberculosis (MAP) recombinant proteins upregulated under stress conditions are immunogenic in sheep. Vet Immunol Immunop 2012; 149:186-196. 3. Cossu D, Otsubo S, Otsubo Y, Eda S, Suzuki T, Iwao Y, Kuribayashi T, Yamamoto S, Sechi L, Momotani E. Mycobacterium avium Subsp. paratuberculosis Induces Specific IgE Production in Japanese People with Allergies. Int J Inflam 2017. 4. Dow CT, Ellingson JL. Detection of Mycobacterium avium ss. paratuberculosis in Blau syndrome tissues. Autoimmune Dis 2010; 2011:127692. 5. Cossu A, Rosu V, Paccagnini D, Cossu D, Pacifico A, Sechi LA. MAP3738c and MptD are specific tags of Mycobacterium avium subsp. paratuberculosis infection in type I diabetes mellitus.Clin Immunol 2011;141:49-57. 6. Pinna A, Masala S, Blasetti F, Maiore I, Cossu D, Paccagnini D, Mameli G, Sechi LA. Detection of serum antibodies cross-reacting with Mycobacterium avium subspecies paratuberculosis and beta-cell antigen zinc transporter 8 homologous peptides in patients with high-risk proliferative diabetic retinopathy. PloS One 2014; 9:e107802. 7. Cossu D, Cocco E, Paccagnini D, Masala S, Ahmed N, Frau J, Marrosu MG, Sechi LA. Association of Mycobacterium avium subsp. paratuberculosis with multiple sclerosis in Sardinian patients. PLoS One 2011;6:e18482. 8. Juste RA. Slow infection control by vaccination: paratuberculosis. Vet Immunol Immunopathol 2012;148:190-196. 9. Park H-T, Yoo HS. Development of vaccines to Mycobacterium avium subsp. paratuberculosis infection. Clin Exp Vaccine Res 2016;5:108-116. 10. Rosseels V, Huygen K. Vaccination against paratuberculosis. Expert Rev Vaccines 2008;7:817-832. 11. Lefrancois LH, Bodier CC, Cochard T, Canepa S, Raze D, Lanotte P, Sevilla IA, Stevenson K, Behr MA, Locht C. Novel feature of Mycobacterium avium subsp. paratuberculosis, highlighted by characterization of the heparin-binding hemagglu-tinin adhesin. J Bacteriol 2013;195:4844-4853. 12. Pethe K, Bifani P, Drobecq H, Sergheraert C, Debrie A-S, Locht C, Menozzi FD. Mycobacterial heparin-binding hemagglutinin and laminin-binding protein share antigenic methyllysines that confer resistance to proteolysis. Proc Natl Acad Sci USA 2002;99:10759-10764. 13. Masungi C, Temmerman S, Van Vooren J-P, Drowart A, Pethe K, Menozzi FD, Locht C, Mascart F. Differential T and B cell responses against Mycobacterium tuberculosis heparin-binding hemagglutinin adhesin in infected healthy individuals and patients with tuberculosis. Jpn J Infect Dis 2002;185:513-520. 14. Parra M, Pickett T, Delogu G, Dheenadhayalan V, Debrie A-S, Locht C, Brennan MJ. The mycobacterial heparin-binding hemagglutinin is a protective antigen in the mouse aerosol challenge model of tuberculosis. Infect Immun 2004;72:6799-6805. 15 Secott T, Lin T, Wu C. Mycobacterium avium subsp. paratuberculosis fibronectin attachment protein facilitates M-cell targeting and invasion through a fibronectin bridge with host integrins. Infect Immun 2004;72:3724-3732. 16. Lee JS, Shin SJ, Collins MT, Jung ID, Jeong Y-I, Lee C-M, Shin YK, Kim D, Park Y-M. Mycobacterium avium subsp. paratuberculosis fibronectin attachment protein activates dendritic cells and induces a Th1 polarization. Infect Immun 2009; 77:2979-2988. 17. Krainer FW, Dietzsch C, Hajek T, Herwig C, Spadiut O, Glieder A. Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway. Microb Cell Fact 2012;11:22. 18. Daly R, Hearn MT. Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. J Mol Recognit 2005;18: 119-138. 19. Eckart MR, Bussineau CM. However, further studies are needed to check the potential of the designed fusion protein in the activation of cell mediated immunity in animal models. Curr Opin Biotechnol 1996;7:525-530. 20. Chen X, Zaro JL, Shen W-C. Fusion protein linkers: property, design and functionality. Adv Drug Deliver Rev 2013;65:1357-1369. 21. Zhang J, Yun J, Shang Z, Zhang X, Pan B. Design and optimization of a linker for fusion protein construction. Prog Nat Sci 2009;19:1197-1200. 22. Zhang Y, Skolnick J. TM-align: a protein structure alignment algorithm based on the TM-score. Nucleic Acids Res 2005;33:2302-2309. 23. Koets A, Rutten V, Hoek A, Bakker D, Van Zijderveld F, Müller K, Van Eden W. Heat-shock protein-specific T-cell responses in various stages of bovine paratuberculosis. Vet Immunol Immunopathol 1999;70:105-115. 24. Shin SJ, Chang C-F, Chang C-D, McDonough SP, Thompson B, Yoo HS, Chang Y-F. In vitro cellular immune responses to recombinant antigens of Mycobacterium avium subsp. paratuberculosis. Infect Immun 2005;73:5074-5085. 25. Huntley JF, Stabel JR, Bannantine JP. Immunoreactivity of the Mycobacterium avium subsp. paratuberculosis 19-kDa lipoprotein. BMC Microbiol 2005;5:3. 26. Rigden RC, Jandhyala DM, Dupont C, Crosbie-Caird D, Lopez-Villalobos N, Maeda N, Gicquel B, Murray A. Humoral and cellular immune responses in sheep immunized with a 22 kilodalton exported protein of Mycobacterium avium subspecies paratuberculosis. J Med Microbiol 2006;55:1735-1740. 27. Nagata R, Muneta Y, Yoshihara K, Yokomizo Y, Mori Y. Expression cloning of gamma interferon-inducing antigens of Mycobacterium avium subsp. Paratubercu-losis. Infect Immun 2005;73:3778-3782. 28. Olsen I, Reitan LJ, Holstad G, Wiker HG. Alkyl hydroperoxide reductases C and D are major antigens constitutively expressed by Mycobacterium avium subsp. paratuberculosis. Infect Immun 2000;68:801-808. 29. Lefrancois LH, Bodier CC, Lecher S, Gilbert FB, Cochard T, Harichaux G, Labas V, Teixeira-Gomes AP, Raze D, Locht C. Purification of native HBHA from Mycobacterium avium subsp. paratuberculosis. BMC Res Notes 2013;6:55. 30. Menozzi FD, Bischoff R, Fort E, Brennan MJ, Locht C. Molecular characterization of the mycobacterial heparin-binding hemagglutinin, a mycobacterial adhesin. Proc Natl Acad Sci USA 1998;95:12625-12630. 31. Noh KT, Shin SJ, Son KH, Jung ID, Kang HK, Lee SJ, Lee EK, Shin YK, You JC, Park Y-M. The Mycobacterium avium subsp. paratuberculosis fibronectin attachment protein, a toll-like receptor 4 agonist, enhances dendritic cell-based cancer vaccine potency. Exp Mol Med 2012;44:340-349. 32. Laqueyrerie A, Militzer P, Romain F, Eiglmeier K, Cole S, Marchal G. Cloning, sequencing, and expression of the apa gene coding for the Mycobacterium tuberculosis 45/47-kilodalton secreted antigen complex. Infect Immun 1995;63: 4003-4010. 33. Romain F, Laqueyrerie A, Militzer P, Pescher P, Chavarot P, Lagranderie M, Auregan G, Gheorghiu M, Marchal G. Identification of a Mycobacterium bovis BCG 45/47-kilodalton antigen complex, an immunodominant target for antibody response after immunization with living bacteria. Infect Immun 1993;61:742-750. 34. Beatty WL, Russell DG. Identification of mycobacterial surface proteins released into subcellular compartments of infected macrophages. Infect Immun 2000;68: 6997-7002. 35. Holsti MA, Schorey JS, Brown EJ, Allen PM. Identification of epitopes of fibronectin attachment protein (FAP-A) of Mycobacterium avium which stimulate strong T-cell responses in mice. Infect Immun 1998;66:1261-1264. | ||
|
آمار تعداد مشاهده مقاله: 3,057 تعداد دریافت فایل اصل مقاله: 4,715 |
||