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Assessment of genetic diversity of an endangered tooth-carp, Aphanius farsicus (Teleostei: Cyprinodontiformes: Cyprinodontidae) using microsatellite markers | ||
| Molecular Biology Research Communications | ||
| مقاله 1، دوره 6، شماره 4، 2017، صفحه 153-160 اصل مقاله (262.15 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2017.24404.1246 | ||
| نویسندگان | ||
| Sareh Yaripour1؛ Hamid Reza Esmaeili1؛ Ali Gholamhosseini* 1؛ Mohammad Rezaei2؛ Saber Sadeghi1 | ||
| 1Zoology Section, Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran | ||
| 2Fishery Faculty, Gorgan University of Agriculture Science and Natural Resources, Gorgan, Iran | ||
| چکیده | ||
| Genetic structure of an endemic tooth-carp fish, Aphanius farsicus from four different water bodies in the Maharlu Lake basin was investigated by applying five microsatellite markers. All of the five examined microsatellite loci showed polymor-phism pattern. A total of four alleles were detected at five microsatellite loci, with an average of 2.8 to 3.5 alleles per locus. Average values of observed and expected heterozygosity were 0.95±0.09 and 0.64±0.02 respectively. None of the tests of linkage disequilibrium were significant between each pair of loci and no deviation from Hardy-Weinberg equilibrium were detected to test for heterozygote deficiency within populations. The Nei's genetic distance values ranged between 0.03 – 0.13. Analysis of pairwise genetic differentiation between each pair of the populations revealed that fixation index (FST) values ranged from 0.013 to 0.039 and RST ranged from 0.005 to 0.065. High genetic diversity observed within the populations (99%) and low diversity (1%) among them indicating probably high level of gene flow among the studied populations of Fars tooth-carp at the present time or in the past. Regarding low genetic differentiation among the studied populations and results of population assignment test, two hypotheses are suggested and supporting evidence for each hypothesis are provided. | ||
| کلیدواژهها | ||
| Maharlu Lake؛ Genetic differentiation؛ Microsatellite؛ Tooth-carp fishes | ||
| مراجع | ||
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