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LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 11، دوره 18، شماره 2 - شماره پیاپی 59، 2017، صفحه 134-137 اصل مقاله (282.89 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2017.4099 | ||
| نویسندگان | ||
| R. Deb* 1؛ G. S. Sengar* 2؛ U. Singh1؛ S. Kumar1؛ T. V. Raja1؛ R. Alex1؛ R. R. Alyethodi1؛ B. Prakash1 | ||
| 1Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut-250 001, Uttar Pradesh, India | ||
| 2MSc in Biotechnology, Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut-250 001, Uttar Pradesh, India | ||
| چکیده | ||
| Animal species detection is one of the crucial steps for consumer’s food analysis. In the present study we developed an in-house built loop-mediated isothermal amplification (LAMP) assay for rapid detection of adulterated cow DNA in goat milk/meat samples. The cow milk/tissue DNA in goat milk/meat samples were identified in the developed LAMP assay by either naked eye visualizing with SYBR Green I dyes or by detecting the typical ladder pattern on gel electrophoresis. This test can detect up to minimum 5% level of cow components admixed in goat milk/meat samples and can be completed within 1 h 40 min starting from DNA extraction from milk/meat samples and can be performed in a water bath. Developed LAMP methodology is simple; rapid and sensitive techniques that can detect adulterant like cow components in goat milk/meat are more accurate than other existing DNA based technologies. | ||
| کلیدواژهها | ||
| Cow؛ Goat؛ LAMP assay؛ Meat؛ Milk | ||
| مراجع | ||
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