| تعداد نشریات | 24 |
| تعداد شماره‌ها | 934 |
| تعداد مقالات | 8,383 |
| تعداد مشاهده مقاله | 16,981,302 |
| تعداد دریافت فایل اصل مقاله | 14,371,042 |
Detection of specific antigens of Newcastle disease virus using an absorbed Western blotting method | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 3، دوره 18، شماره 2 - شماره پیاپی 59، 2017، صفحه 92-96 اصل مقاله (243.42 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2017.4087 | ||
| نویسندگان | ||
| F. Hemmatzadeh1؛ M. Kazemimanesh* 2 | ||
| 1School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, Australia | ||
| 2School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, Australia; 2Department of Virology, Pasteur Institute of Iran, Tehran, Iran | ||
| چکیده | ||
| Newcastle disease virus (NDV) is an economically important poultry pathogen with a worldwide distribution that may infect a wide range of domestic and wild avian species. The identification of different pathotypes of NDVs plays an important role in the diagnosis and development of vaccines to control and eradicate NDV infections. In our previous study, we showed that mono-specific antibodies can differentiate velogenic and lentogenic strains of NDV in Agar Gel Immuno-Diffusion tests. To evaluate the ability of the specific antibodies to detect NDV specific antigens, this study was conducted with a range of NDV isolates. The samples included 9 NDV neuropathogenic/velogenic isolates from diseased chickens collected from poultry farms in central and northern parts of Iran plus La-Sota and B1 vaccine strains. All samples were propagated in embryonated chicken eggs and concentrated and purified by ultra-centrifugation. All samples were subjected to 12.5% SDS-PAGE and Western blotting using the specific antibodies mentioned previously. In SDS-PAGE all velogenic and vaccine strains showed the same electrophoretic pattern. The detected bands included 15, 38, 46, 48, 53, 55, 68, 74 and 220 kDa proteins. In Western blotting analysis, the mono-specific antibodies reacted specifically to the viral proteins with 15, 38, 48, 55, 74 and 220 kDa and non-specifically to the viral protein with 53 kDa. The results suggest that specific anti-NDV antibodies can react specifically to glycoproteins (haemagglutin-neuraminidase and fusion proteins) but not to internal proteins (nucleoprotein or matrix protein) of NDV strains. | ||
| کلیدواژه‌ها | ||
| Newcastle disease virus؛ Specific antigens؛ Western blotting method | ||
| مراجع | ||
|
Afonso, CL; Miller, PJ; Grund, Ch and Koch, GBP (2012). Manual of diagnostic tests and vaccines for terrestrial animals. 7th Edn., Vol. 1, World Organization for Animal Health (OIE), Paris, France. PP: 555-576.
Alexander, D (1988). Newcastle disease diagnosis. In: Newcastle disease. USA, Springer. PP: 147-160. Alexander, D (2000). Newcastle disease and other avian paramyxoviruses. Rev. Sci. Tech., 19: 443-462. Dortmans, J; Rottier, P; Koch, G and Peeters, B (2010). The viral replication complex is associated with the virulence of Newcastle disease virus. J. Virol., 84: 10113-10120. Dufour-Zavala, L (2008). A laboratory manual for the isolation, identification, and characterization of avian pathogens. 4th Edn., AAAP. PP: 221-230. Garcia, SC; Lopez, RN; Morales, R; Olvera, MA; Marquez, MA; Merino, R; Miller, PJ and Afonso, CL (2013). Molecular epidemiology of Newcastle disease in Mexico and the potential spillover of viruses from poultry into wild bird species. Appl. Environ. Microbiol., 79: 4985-4992. Gotoh, B; Ohnishi, Y; Inocencio, N; Esaki, E; Nakayama, K; Barr, P; Thomas, G and Nagai, Y (1992). Mammalian subtilisin-related proteinases in cleavage activation of the paramyxovirus fusion glycoprotein: superiority of furin/PACE to PC2 or PC1/PC3. J Virol., 66: 6391-6397. Heiden, S; Grund, C; Röder, A; Granzow, H; Kühnel, D; Mettenleiter, TC and Römer-Oberdörfer, A (2014). Different regions of the Newcastle sisease virus fusion protein modulate pathogenicity. PLoS One. 9: e113344. Hemmatzadeh, F and Alinejad, A (2003). A study on proteinal pattern of isolated NDV in Iran and comparison with live vaccine strains. Iran. J. Vet. Res., 58: 61-65. Hemmatzadeh, F; Nayeri, B and Tofighi, E (2006). Antigenic differences in Newcastle disease viruses isolated in Iran. Int. J. Poult. Sci., 5: 408-410. Hemmatzadeh, F; Nikbakht-Brojeni, G; Kateb, S; Mokhtari, F; Alinejad, A and Ghafari, M (2005). Designing of a specific immunoflurescence method for differentiation of velogenic and vaccinal strains of Newcastle disease virus. Iran. J. Vet. Res., 60: 393-399. Hemmatzadeh, F and Sharifzadeh, A (2006). A specific dot-blot immunoassay for Newcastle disease serodiagnosis. Int. J. Poult. Sci., 5: 1079-1081. Laemmli, UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685. Maclachlan, NJ; Dubovi, EJ and Fenner, F (2010). Fenner’s veterinary virology. 4th Edn., Amsterdam, Boston, Elsevier Academic Press. PP: 264-266. Mahy, BW and Kangro, HO (1996). Virology methods manual. 1st Edn., Vol. 32, UK, Academic Press London. PP: 71-90.
Nagai, Y; Klenk, HD and Rott, R (1976). Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology. 72: 494-508. Ogasawara, T; Gotoh, B; Suzuki, H; Asaka, J; Shimokata, K; Rott, R and Nagai, Y (1992). Expression of factor X and its significance for the determination of paramyxovirus tropism in the chick embryo. EMBO J., 11: 467-472. Panda, A; Huang, Z; Elankumaran, S; Rockemann, DD and Samal, SK (2004). Role of fusion protein cleavage site in the virulence of Newcastle disease virus. Microb. Pathog., 36: 1-10. Römer-Oberdörfer, A; Werner, O; Veits, J; Mebatsion, T and Mettenleiter, TC (2003). Contribution of the length of the HN protein and the sequence of the F protein cleavage site to Newcastle disease virus pathogenicity. J. Gen. Virol., 84: 3121-3129. Sambrook, J; Russell, DW and Russell, DW (2001). Molecular cloning: a laboratory manual (3-volume set). Steward, M; Vipond, IB; Millar, NS and Emmerson, PT (1993). RNA editing in Newcastle disease virus. J. Gen. Virol., 74: 2539-2548.
Tseung, H; Lai, C; Lin, D; Lin, Y; Chen, C; Lian, W and Chen, W (1993). Purification of envelope glycoproteins of the Newcastle disease virus. J. Chin. Soi. Vet. Sci., 19: 11-18. | ||
|
آمار تعداد مشاهده مقاله: 1,328 تعداد دریافت فایل اصل مقاله: 956 |
||