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khalouie, Farzane, Mousavi, Seyed Latif, Nazarian, Shahram, Amani, Jafar, Pourfarzam, Poune. (1396). Immunogenic evaluation of chimeric recombinant protein against ETEC, EHEC and Shigella. , 6(3), 101-112. doi: 10.22099/mbrc.2017.4081
Farzane khalouie; Seyed Latif Mousavi; Shahram Nazarian; Jafar Amani; Poune Pourfarzam. "Immunogenic evaluation of chimeric recombinant protein against ETEC, EHEC and Shigella". , 6, 3, 1396, 101-112. doi: 10.22099/mbrc.2017.4081
khalouie, Farzane, Mousavi, Seyed Latif, Nazarian, Shahram, Amani, Jafar, Pourfarzam, Poune. (1396). 'Immunogenic evaluation of chimeric recombinant protein against ETEC, EHEC and Shigella', , 6(3), pp. 101-112. doi: 10.22099/mbrc.2017.4081
khalouie, Farzane, Mousavi, Seyed Latif, Nazarian, Shahram, Amani, Jafar, Pourfarzam, Poune. Immunogenic evaluation of chimeric recombinant protein against ETEC, EHEC and Shigella. , 1396; 6(3): 101-112. doi: 10.22099/mbrc.2017.4081

Immunogenic evaluation of chimeric recombinant protein against ETEC, EHEC and Shigella

Molecular Biology Research Communications
مقاله 2، دوره 6، شماره 3، 2017، صفحه 101-112    اصل مقاله (280.44 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2017.4081
نویسندگان
Farzane khalouie1؛ Seyed Latif Mousavi* 1؛ Shahram Nazarian2؛ Jafar Amani3؛ Poune Pourfarzam1
1Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.
2Department of Biology, Faculty of Sciences, Imam Hossein University, Tehran, Iran.
3Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
چکیده
Diarrheal diseases still remain health problem worldwide and out of many bacteria responsible for, Shigella and pathogenic Escherichia cause the most diarrheas in the world. Shigellacause bacterial dysenteries and shigellosis through invasion where the most effective proteins for pathogenesis is Ipac. Critical virulence protein for ETEC infection is CFA/I with two subunits called cfab and cfae. . Attachment of EHEC is the main step of infection and the protein Intimin plays the key role in this function.  Protection against the vast majority of responsible pathogens of diarrheas requires development of the combination vaccine against Shigella, ETEC and EHEC. In the present study, a multisubunitprotein (CII) containing immunologically significant parts of CfaB, IpaC and Intimin was designed. The chimeric gene (CII) was codon optimized and analyzed with different bioinformatic servers, then synthesized and expressed in E. coli. Mice, Guinea pig and, Caco-2 Cell line were used as challenge models for EHEC, shigella and ETEC respectively. The chimeric protein induced significant immune response and therefore could be a suitable vaccine candidate against these three pathogens.
کلیدواژه‌ها
Chimeric vaccine؛ IpaC؛ CFaB؛ Intimin
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