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Cloning and expression of fragment of the rabies virus nucleoprotein gene in Escherichia coli and evaluation of antigenicity of the expression product | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 7، دوره 18، شماره 1 - شماره پیاپی 58، 2017، صفحه 36-42 اصل مقاله (277.7 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2017.4028 | ||
| نویسندگان | ||
| K. Tursunov* 1؛ A. Begaliyeva1؛ B. Ingirbay1؛ K. Mukanov1؛ E. Ramanculov2؛ A. Shustov3؛ K. Mukantayev1 | ||
| 1Department of Immunochemistry and Immunobiotechnology, National Center for Biotechnology, Astana 010000, Kazakhstan | ||
| 2National Center for Biotechnology, Astana 010000, Kazakhstan | ||
| 3Department of Genetic Engineering, National Center for Biotechnology, Astana 010000, Kazakhstan | ||
| چکیده | ||
| Rabies virus nucleoprotein (N protein) encapsidates genomic RNA of the virus and forms the viral ribonucleoprotein complex. These N proteins represent highly organized structures which activate proliferation of B cells and production antibodies against the N protein. In addition to the B cell, the rabies virus N protein has been shown to induce potent T helper cell responses resulting in a long-lasting and strong humoral immune response. Rabies virus N protein is a molecular target of choice for development of tools to diagnose acute rabies infection. We produced a recombinant immune reactive C-terminal fragment of the rabies virus N protein which contains an antigenic determinant located between positions 360-389. Synthetic gene encoding the N protein was cloned into an expression plasmid to produce the recombinant antigen in Escherichia coli cells BL21 (DE3). SDS-PAGE showed presence of the product with expected molecular weight (44 kDa). The recombinant fragment of the N protein efficiently recognized antibodies in sera from mice immunized with an inactivated rabies virus. Thus produced recombinant antigen of the rabies virus N protein can be used in an enzyme-linked immunosorbent assay (ELISA) for diagnosis of the rabies infection. | ||
| کلیدواژهها | ||
| Diagnostics؛ ELISA؛ Nucleoprotein؛ Rabies virus؛ Recombinant antigen | ||
| مراجع | ||
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Akihiko, K; Harufusa, T; Tadafumi, ST; Takuo, T; Yoshikazu, H and Kinjiro, M (1999). Nucleocapsid formation and/or subsequent conformational change of rabies virus nucleoprotein (N) is a prerequisite step for acquiring the phosphatase-sensitive epitope of monoclonal antibody. Virology. 263: 395-407.
Dietzschold, B; Lafon, M; Wang, H; Otvos, LJr; Celis, E; Wunner, WH and Koprowski, H (1987). Localization and immunological characterization of antigenic domains of the rabies virus internal N and NS proteins. Virus Res., 8: 103-125.
Fanayi, K; Ajorloo, M; Mozhgani, SHR; Irani, S and Gholami, A (2014). Design, construction and expression of recombinant vector containing the rabies virus nucleo-protein gene. TehranUniv. Med. J., 72: 294-300.
Flamand, A; Wiktor, TJ and Koprowski, H (1980). Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. I. The nucleocapsid protein. J. Gen. Virol., 48: 97-104.
Goto, H; Minamoto, N; Ito, H; Luo, TR; Sugiyama, M; Kinjo, T and Kavai, A (1995). Expression of the nucleo-protein of rabies virus in Escherichia coli and mapping of antigenic sites. Arch. Virol., 140: 1061-1074.
Guy, S; Frederic, I; Manos, M; Danielle, B and Rob, WHR (2001). Structure of recombinant rabies virus nucleoprotein -RNA complex and identification of the phosphoprotein binding site. J. Virol., 75: 490-498.
Hiroyuki, F; Yoshiko, T; Kumato, M; Kazuhiro, H; Akira, N and Kazuaki, M (1994). Protective efficacy in mice of post-exposure vaccination with vaccinia virus recombinant expressing either rabies virus glycoprotein or nucleo-protein. J. Gen. Virol., 75: 1339-1344.
Inoue, S; Motoi, Y; Kashimura, T; Ono, K and Ya-Mada, A (2003). Safe and easy monitoring of anti-rabies anti-body in dogs using his-tagged recombinant N-protein. Jpn. J. Infect. Dis., 56: 158-160.
Katayama, S; Yamanaka, M; Ota, S and Shimizu, YA (1999). New quantitative method for rabies virus by detection of nucleoprotein in virion using Elisa. J. Vet. Med. Sci., 61: 411-416.
Lafon, M and Wiktor, TJ (1985). Antigenic sites on the ERA rabies virus nucleoprotein and non-structural protein. J. Gen. Virol., 66: 2125-2133. Manjunatha, RGB; Singh, R; Singh, RP; Singh, KP; Gupta, PK; Anita, D; Shankar, SK; Ramakrishnan, MA and Rishendra, V (2011). Molecular characterization of Indian rabies virus isolates by partial sequencing of nucleoprotein (N) and phosphoprotein (P) genes. Virus Genes. 43: 13-17.
Masatani, T; Ito, N; Shimizu, K; Ito, Y; Nakagawa, K; Abe, M; Yamaoka, S and Sugiyama, M (2011). Amino acids at positions 273 and 394 in rabies virus nucleoprotein are important for both evasion of host RIG-I-mediated antiviral response and pathogenicity. Virus Res., 155: 168-174.
Masatani, T; Naoto, I; Kenta, S; Yuki, I; Keisuke, N; Yoshiharu, S; Hiroyuki, K and Makoto, S (2010). Rabies virus nucleoprotein functions to evade activation of the RIG-I-mediated antiviral response. J. Virol., 84: 4002-4012.
Minamoto, N; Tanaka, H; Hishida, M; Goto, H; Ito, H; Naruse, S; Yamamoto, K; Sugiyama, M; Kinjo, T; Mannen, K and Mifune, K (1994). Linear and conformation-dependent antigenic sites on the nucleoprotein of rabies virus. Microbiol. Immunol., 38: 449-455.
Wangbin, C; Ying, H; Qiumei, S; Cairan, Y and Yanying, Z (2013). Expression of nucleoprotein gene of CTN strain rabies virus from China in E. coli with antigenicity. Open J. Vet. Med., 3: 309-313. Xiangping, Y; Zhiyong, L; Jiangtao, L; Yongzhu, Y; Yun, Z; Xuerui, L; Baoyu, L; Bin, Y; Xi, L; Yinv, L; Wenqiang, J; Zhifang, Z and Jixing, L (2013). Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice. J. Biotechnol., 163: 333-338.
Yan, J; Yong, HL; Frank, M; Robert, JH; Ying, H and Zhen, FF (2010). Characterization of conformation-specific monoclonal antibodies against rabies virus nucleo-protein. Arch. Virol., 155: 1187-1192. | ||
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