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Cloning and expression of fragment of the rabies virus nucleoprotein gene in Escherichia coli and evaluation of antigenicity of the expression product | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 7، دوره 18، شماره 1 - شماره پیاپی 58، 2017، صفحه 36-42 اصل مقاله (277.7 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2017.4028 | ||
| نویسندگان | ||
| K. Tursunov* 1؛ A. Begaliyeva1؛ B. Ingirbay1؛ K. Mukanov1؛ E. Ramanculov2؛ A. Shustov3؛ K. Mukantayev1 | ||
| 1Department of Immunochemistry and Immunobiotechnology, National Center for Biotechnology, Astana 010000, Kazakhstan | ||
| 2National Center for Biotechnology, Astana 010000, Kazakhstan | ||
| 3Department of Genetic Engineering, National Center for Biotechnology, Astana 010000, Kazakhstan | ||
| چکیده | ||
| Rabies virus nucleoprotein (N protein) encapsidates genomic RNA of the virus and forms the viral ribonucleoprotein complex. These N proteins represent highly organized structures which activate proliferation of B cells and production antibodies against the N protein. In addition to the B cell, the rabies virus N protein has been shown to induce potent T helper cell responses resulting in a long-lasting and strong humoral immune response. Rabies virus N protein is a molecular target of choice for development of tools to diagnose acute rabies infection. We produced a recombinant immune reactive C-terminal fragment of the rabies virus N protein which contains an antigenic determinant located between positions 360-389. Synthetic gene encoding the N protein was cloned into an expression plasmid to produce the recombinant antigen in Escherichia coli cells BL21 (DE3). SDS-PAGE showed presence of the product with expected molecular weight (44 kDa). The recombinant fragment of the N protein efficiently recognized antibodies in sera from mice immunized with an inactivated rabies virus. Thus produced recombinant antigen of the rabies virus N protein can be used in an enzyme-linked immunosorbent assay (ELISA) for diagnosis of the rabies infection. | ||
| کلیدواژهها | ||
| Diagnostics؛ ELISA؛ Nucleoprotein؛ Rabies virus؛ Recombinant antigen | ||
| مراجع | ||
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