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Molecular identification and successful treatment of Chlamydophila psittaci (genotype B) in a clinically affected Congo African grey parrot (Psittacus erithacus erithacus) | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 12، دوره 17، شماره 4 - شماره پیاپی 57، 2016، صفحه 281-285 اصل مقاله (354.67 K) | ||
| نوع مقاله: Scientific report | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2016.3918 | ||
| نویسندگان | ||
| J. Razmyar* 1؛ M. Rajabioun1؛ M. Zaeemi1؛ A. Afshari2 | ||
| 1Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 2Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran | ||
| چکیده | ||
| Avian chlamydiosis is caused by Chlamydiophila psittaci with the highest infection rate in parrots (Psittacidae) and pigeons (Columbiformes). A two-year-old Congo African grey parrot was examined since the bird had shown clinical signs of anorexia, depression, diarrhea, and mild dyspnea and based on biochemical and hemathological analysis the bird was diagnosed as having anemia, leukocytosis, heterophilia, lymphopenia and monocytosis. With regards to clinical and paraclinical findings, the case was diagnosed to be carrying Chlamydiophila spp. In addition, choanal cleft and cloaca swabs were positive for Chlamydiophila spp. in a diagnostic polymerase chain reaction (PCR) (600 bp amplicon). Polymerase chain reaction products were typed by ompA gene-based PCR, using CTU/CTL primers (1050 bp amplicon). The PCR product sequence was compared with the sequences obtained from GenBank. The phylogenetic tree has revealed 100% identity with genotype B obtained from previous studies. The bird was hospitalized and treated with doxycycline regimen for 45 days, with a weekly sampling process to trace the presence of C. psittaci DNA in faecal and choanal swabs, this process continued to the point where the specimens turned negative after two weeks. Laboratory and radiology results were within normal limits after the treatment. Genotype B is predominantly isolated from Columbidae and there have not been any reports regarding the clinically affected African gray parrot with this genotype. Subsequently, to the best of our knowledge, this is the first report of chlamydiosis by genotype B on Congo African grey parrot. | ||
| کلیدواژهها | ||
| Avian chlamydiosis؛ Chlamydophila psittaci genotype B؛ Congo African grey parrot؛ ompA gene؛ PCR | ||
| مراجع | ||
|
Andersen, AA (1997). Two new serovars of Chlamydia psittaci from North American birds. J. Vet. Diagnost. Inves., 9: 159-164.
Andersen, AA and Vanrompay, D (2003). Avian chlamydiosis (Psittacosis, Ornithosis). In: Diseases of poultry. 11th Edn., Ames, Iowa, USA, IowaStateUniversity Press. PP: 863-879.
Arraiz, N; Bermudez, V; Urdaneta, B; Mujica, E; Sanchez, MP; Mejía, R; Prieto, C; Escalona, C and Mujica, A (2012). Evidence of zoonotic Chlamydophila psittaci transmission in a population at risk in Zulia state, Venezuela. Rev. Panam. Salud. Publica. (Bogota). 14: 305-314.
Bush, RM and Everett, K (2001). Molecular evolution of the chlamydiaceae. Int. J. Syst. Evol. Microbiol., 51: 203-220.
Campbell, TW and Ellis, CK (2007). Avian and exotic animal hematology and cytology. 3rd Edn., Ames, Iowa, Blackwell Pub., PP: 326-328.
Fudge, AM (2000). Avian liver and gastrointestinal testing. In: Fudge, (Ed.), Laboratory medicine avian and exotic pets. (1st Edn.), USA, Saunders Pub., PP: 47-55.
Geens, T; Desplanques, A; Van Loock, M;Bönner, BM; Kaleta, EF; Magnino, S; Andersen, A;Everett, KDA andVanrompay, D (2005). Sequencing of the Chlamydo-phila psittaci ompA gene reveals a new genotype, E/B, and the need for a rapid discriminatory genotyping method. J. Clin. Microbiol., 43: 2456-2461.
Harcourt-Brown, N and Chitty, J (2005). BSAVA manual of psittacine birds. 2nd Edn., Wareham, UK, BSAVA Publications. P: 69.
Heddema, ER;Van Hannen, EJ; Duim, B;Vandenbroucke-Grauls, CMJE andPannekoek, Y (2006). Genotyping of Chlamydophila psittaci in human samples. Emerg. Infect. Dis., 12: 1989-1990.Kaleta, EF and Taday, EM (2003). Avian hosts range of Chlamydophila spp. based on isolation, antigen detection, and serology. Avian Pathol., 32: 435-462.
Madani. SA and Peighambari, SM (2013). PCR-based diagnosis, molecular characterization and detection of atypical strains of avian Chlamydia psittaci in companion and wild birds. Avian Pathol., 42: 38-44.
Natt, MP and Herrick, CA (1952). A new blood diluent for counting the erythrocytes and leucocytes of the chicken. Poult. Sci., 31: 735-738.
OIE, A (2008). Manual of diagnostic tests and vaccines for terrestrial animals. Office International des Epizooties, Paris, France. PP: 1092-1106.
Piasecki, T; Chrząstek, K and Wieliczko, A (2012). Detection and identification of Chlamydophila psittaci in asymptomatic parrots in Poland. BMC. Vet. Res., 8: 233-239.
Pickett, MA; Everson, JS and Clarke, IN (1988). Chlamydia psittaci ewe abortion agent: complete nucleotide sequence of the major outer membrane protein gene. FEMS. Microbiol. Lett., 55: 229-234.
Read, TD;Joseph, SJ; Didelot, X; Liang, B;Patel, L and Dean, D (2013). Comparative analysis of Chlamydia psittaci genomes reveals the recent emergence of a pathogenic lineage with a broad host range. MBio., 4: e00604-00612. Sachse, K and Hotzel, H (2003). Detection and differentiation of Chlamydiae by nested PCR. In: Sachse, K and Frey, J (Eds.), PCR detection of microbial pathogens. (1st Edn.), New Jersey, USA, Humana Press. PP: 123-136.
Sachse, K; Laroucau, K;Hotzel, H;Schubert, E; Ehricht, R and Slickers, P (2008). Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes. BMC. Microbiol., 8: 63-75.
Schachter, J (1999). Infection and disease epidemiology. In: Stephens, RS (Ed.), Chlamydia: intracellular biology, pathogenesis, and immunity. Washington, D.C., USA, ASM Press. PP: 139-169.
West, A (2011). A brief review of Chlamydophila psittaci in birds and humans. J. Exot. Pet. Med., 20: 18-20.
Zhang, YX; Morrison, SJ; Caldwell, HD and Baehr, W (1989). Cloning and sequence analysis of the major outer membrane protein genes of two Chlamydia psittaci strains. Infect. Immun., 57: 1621-1625. | ||
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