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Isolation of Clostridium difficile and molecular detection of binary and A/B toxins in faeces of dogs | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 10، دوره 17، شماره 4 - شماره پیاپی 57، 2016، صفحه 273-276 اصل مقاله (271.33 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2016.3916 | ||
| نویسندگان | ||
| M. Ghavidel1؛ H. Salari Sedigh* 2؛ J. Razmyar2 | ||
| 1Ph.D. Student in Bacteriology, Department of Microbiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 2Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده | ||
| The aim of this study was to isolate Clostridium difficile from dogs’ faeces, and to study the frequency of its virulence genes. A total of 151 samples of dogs’ faeces were collected. The isolation of C. difficile was performed by using the bacterial culture methods followed by DNA extraction using boiling method. Multiplex PCR method was performed for identification of tcdA, tcdB, cdtA and cdtB genes and single method was carried out for detection of tcdC. Twelvesamples (7.9%) were positive in bacteriological assay and based on molecular assay, 66.7% of the isolates (8 of 12 C. difficile isolated) had shown tcdA+, tcdB+ profile. This is the first investigation on molecular assay of C. difficilein Iran’s dog population. | ||
| کلیدواژهها | ||
| Clostridium difficile؛ dog؛ Molecular detection | ||
| مراجع | ||
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