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Effect of doxycycline and Lactobacillus probiotics on mRNA expression of ABCC2 in small intestines of chickens | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 8، دوره 17، شماره 4 - شماره پیاپی 57، 2016، صفحه 265-267 اصل مقاله (258.73 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2016.3913 | ||
| نویسندگان | ||
| A. Milanova* 1؛ I. Pavlova1؛ V. Yordanova2؛ S. Danova3 | ||
| 1Department of Pharmacology, Physiology of Animals and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria | ||
| 2BSc in Molecular Biology, Molecular Diagnostics Unit, Hospital for Active Treatment “Dr. Atanas Dafovski”, Kurdjali, Bulgaria | ||
| 3The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences (BAS), 26, Akad. G. Bontchev, Sofia, Bulgaria | ||
| چکیده | ||
| Probiotics and antibiotics are widely used in poultry and may alter drug bioavailability by affecting the expression of intestinal ATP-binding cassette (ABC) efflux transporters. Therefore the aim of the present investigation was to evaluate the effect of Lactobacilli probiotics, administered alone or in combination with doxycycline, on the expression of ABCB1 (gene, encoding P-glycoprotein), ABCC2 (gene, encoding multidrug resistance protein 2, MRP2) and ABCG2 (gene, encoding breast cancer resistance protein) mRNAs in chicken using RT-PCR. Duc one-day-old chicks (n=24) were divided equally in four groups: untreated control, probiotics supplemented group, probiotics plus doxycycline treated chickens and antibiotic administered group. Expression of ABCC2 mRNA was affected by doxycycline or by combination of Lactobacillus plantarum, L. brevis and L. bulgaricus and the antibiotic in the intestines. These results can be used as a basis for further functional studies to prove the beneficial effect on limitation of the absorption of toxins and improvement of efflux of endogenous substances and xenobiotics when the combination of doxycycline and Lactobacillus spp. probiotics are administered to poultry. | ||
| کلیدواژهها | ||
| ABC transporters؛ Doxycycline؛ Lactobacillus probiotics؛ Poultry | ||
| مراجع | ||
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Angelakis, E; Bastelica, D; Ben Amara, A; El Filali, A; Dutour, A; Mege, JL; Alessi, MC and Raoult, D (2012). An evaluation of the effects of Lactobacillus ingluviei on body weight, the intestinal microbiome and metabolism in mice. Microb. Pathog., 52: 61-68.
Borst, P; Zelcer, N and van de Wetering, K (2006). MRP2 and 3 in health and disease. Cancer Lett., 8: 51-61.
Guo, M; Sun, Y; Zhang, Y; Bughio, S; Dai, X; Ren, W and Wang, L (2014). E. coli infection modulates the pharma-cokinetics of oral enrofloxacin by targeting P-glycoprotein in small intestine and CYP450 3A in liver and kidney of broilers. PLoS One. 9: e87781.
Haritova, AM (2006). PK-PD modeling of fluoroquinolones and ABC transporters in poultry. Ph.D. Thesis, University Utrecht, The Netherlands. PP: 111-144.
Kabir, SM (2009). The role of probiotics in the poultry industry. Int. J. Mol. Sci., 10: 3531-3546.
Pan, D and Yu, Z (2014). Intestinal microbiome of poultry and its interaction with host and diet. Gut Microbes. 5: 108-119.
Pavlova, I (2015). Effect of probiotics on doxycycline disposition in gastro-intestinal tract of poultry. Bulg. J. Vet. Med., 18: 248-257.
Schrickx, JA and Fink-Gremmels, J (2008). Implications of ABC transporters on the disposition of typical veterinary medicinal products. Eur. J. Pharmacol., 13: 510-519.
Stojančević, M; Bojić, G; Salami, HA and Mikov, M (2014). The influence of intestinal tract and probiotics on the fate of orally administered drugs. Curr. Issues Mol. Biol., 16: 55-68.
Su, L; Dong, L; Bughio, S; Guo, M and Wang, L (2014). Effect of colibacillosis or coccidiosis on expression of breast cancer resistance protein in small intestine and liver of chickens. J. Vet. Pharmacol. Ther., 37: 53-58.
Tropcheva, R; Hristova, J; Georgieva, R; Salageanu, A; Sgouras, DN and Danova, S (2013). In vitro assessment of prebiotic utilization by dairy lactobacilli. Bulg. J. Agricult. Sci., 19: 105-107.
Vandesompele, J; Kubista, M and Pfaffl, MW (2009). Reference gene validation software for improved normali-zation. In: Logan, J; Edwards, K and Saunders, N (Eds.), Real-time PCR: current technology and applications. (1st Edn.), London, Caister Academic Press. PP: 47-64.
Wang, Y; Liu, Y; Sidhu, A; Ma, Z; McClain, C and Feng, W (2012). Lactobacillus rhamnosus GG culture super-natant ameliorates acute alcohol-induced intestinal permeability and liver injury. Am. J. Physiol. Gastrointest. Liver Physiol., 303: G32-41.
Yin, J; Zhang, XX; Wu, B and Xian, Q (2015). Metagenomic insights into tetracycline effects on microbial community and antibiotic resistance of mouse gut. Ecotoxicology. 24: 2125-2132. | ||
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