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Developmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 6، دوره 17، شماره 4 - شماره پیاپی 57، 2016، صفحه 253-258 اصل مقاله (222.98 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2016.3911 | ||
| نویسندگان | ||
| T. H. Scholkamy1؛ D. A. El-Badry2؛ K. Gh. M. Mahmoud* 3 | ||
| 1Department of Field Investigations, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt | ||
| 2Department of Artificial Insemination and Embryo Transfer, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt | ||
| 3Department of Animal Reproduction and Artificial Insemination, National Research Center, Dokki, Giza, Egypt | ||
| چکیده | ||
| The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and rinsed for obtaining the sperm rich fluid. Ejaculated and epididymal spermatozoa were processed for cryopreservation. Fresh and frozen-thawed ejaculated and epididymal spermatozoa were evaluated for motility, livability, membrane and acrosomal integrities. Frozen-thawed ejaculated and epididymal spermatozoa were used to fertilize camel mature oocytes in vitro. The results showed that, the progressive motility of freshly collected epididymal spermatozoa was significantly (P<0.05) higher than ejaculated spermatozoa (49.25 ± 1.75 vs. 38.50 ± 1.50%, respectively). The viability index of epididymal spermatozoa was significantly (P<0.05) higher than that of ejaculated spermatozoa (96.63 ± 2.45 vs. 84.00 ± 4.08, respectively). The post-thaw acrosome and membrane integrities of epididymal spermatozoa were significantly (P<0.05) higher than those of ejaculated spermatozoa. Morula and blastocyst rates of camel oocytes in vitro fertilized by frozen-thawed epididymal spermatozoa (59.4 ± 0.8, 19.12 ± 0.7 and 10.29 ± 0.7%, respectively) were significantly (P<0.05) higher than those fertilized by frozen-thawed ejaculated spermatozoa (48.27 ± 3.1, 11.63 ± 1.1 and 5.43 ± 0.8%, respectively). In conclusion, the Dromedary camel frozen epididymal spermatozoa have the capacity to endure cryopreservation, fertilize oocytes and produce embryos in vitro better than ejaculated sperm. | ||
| کلیدواژهها | ||
| Camel؛ Ejaculated semen؛ Epididymal spermatozoa؛ In vitro fertilization | ||
| مراجع | ||
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Abdoon, AS; Kandil, OM; Berisha, B; Kliem, H and Schams, D (2007). Morphology of Dromedary camel oocytes and their ability to spontaneous and chemical parthenogenetic activation. Reprod. Dom. Anim., 42: 88-93.
Abdoon, AS; Kandil, OM; Pizzi, F; Turri, F; El Atrash, A and Sabra, HA (2013). Effect of semen extender on cryopreservation and fertilization rates of Dromedary camel epididymal spermatozoa. Scientific Conference of Camel Research and Production. Khartoum, Sudan, 17th-18th April 2013.
Al-Qarawi, HA; Abdel-Rahman, SA; El-Mougy, MS and El-Belely, AA (2002). Use of a new computerized system for evaluation of spermatozoal motility and velocity characteristics in relation to fertility levels in Dromedary bulls. Anim. Reprod. Sci., 74: 1-9.
Axner, E; Hermansson, U and Linde-Forsberg, C (2004). The effect of Equex STM paste and sperm morphology on post-thaw survival of cat epididymal spermatozoa. Anim. Reprod. Sci., 84: 179-191.
Badr, MR and Abdel-Malak, MG (2010). In vitro fertilization and embryo production in Dromedary camel using epididymal spermatozoa. Global Vet., 4: 271-276.
Barker, CA and Gandier, SCC (1957). Pregnancy in a mare resulting from frozen epididymal spermatozoa. Can. J. Comp. Med., 21: 47-51.
Billah, M and Skidmore, JA (1992). The collection, evaluation and deep-freezing of Dromedary camel semen. In: Proceedings of 1st International Camel Conference. Dubai, UAE, February 2-6. P: 61 (abst.).
Blash, S; Melican, D and Gavin, W (2000). Cryopreservation of epididymal sperm obtained at necropsy from goats. Theriogenology. 54: 899-905.
Brackett, BG and Oliphant, G (1975). Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12: 260-274.
Chaveiro, A; Cerqueira, C; Silva, J; Franco, J and Moreira da Silva, F (2015). Evaluation of frozen thawed cauda epididymal sperms and in vitro fertilizing potential of bovine sperm collected from the cauda epididymal. Iran. J. Vet. Res., 16: 188-193.
Didion, BA; Dobrinsky, JR; Giles, JR and Graves, CN (1989). Staining procedure to detect viability and true acrosome reaction in spermatozoa of various species. Gamete Res., 22: 51-57.
El-Badry, DA; Scholkamy, TH; Anwer, AM and Mahmoud, KGhM (2015). Assessment of freezability and functional integrity of Dromedary camel spermatozoa harvested from caput, corpus and cauda epididymides. Alexandria J. Vet. Sci., 44: 147-158.
El-Sayed, A; Ashour, G; Kamel, AM and El-Bahrawy, KA (2015). Assessment of embryo production of Dromedary (Camelus dromedarius) using two semen sources and two in vitro fertilization techniques. Egypt. J. Anim. Prod., 52: 153-160.
El-Sayed, A; Sayed, HA; El-Hassanein, EE; Murad, H and Barkawi, AH (2012). Effect of epidermal growth factor on in vitro production of camel (Camelus dromedarius) embryos by using frozen semen. Egypt. J. Anim. Prod., 49: 39-45.
Fathi, M; Seida, AA; Sobhy, RR; Darwish, GM; Badr, MR and Moawad, AR (2014). Caffeine supplementation during IVM improves frequencies of nuclear maturation and preimplantation development of Dromedary camel oocytes following IVF. Theriogenology. 81: 1286-1292.
García-Álvarez, O; Maroto-Morales, A; Martínez-Pastor, F; Garde, JJ; Ramón, M; Fernández-Santos, MR; Esteso, MC; Pérez-Guzmán, MD and Soler, AJ (2009). Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: electro-ejaculation and postmortem collection. Theriogenology. 72: 160-168. Hassan, MM; Saed, M and Muqtadir, R (1995). Semen collection by artificial vagina and cryopreservation of camel (Camelus dromederious) spermatozoa. Pak. Vet. J., 15: 105-108. Heise, A; Thompson, PN and Gerber, D (2011). Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa. Anim. Reprod. Sci., 123: 192-201. Jeyendran, RS; Vander-Ven, HH; Perez-Pelaez, M; Crabo, BG and Zanevld, LJD (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characters. J. Repord. Fertil., 70: 219-228.
Kaabi, M; Paz, P; Alvarez, M; Anel, E; Boixo, JC; Rouissi, H; Herraez, P and Anel, L (2003). Effect of epididymis handling conditions on the quality of ram spermatozoa recovered post-mortem. Theriogenology. 60: 1249-1259.
Katska, L; Ryńska, B and Smora, Z (1996). Effect of seminal plasma on the in vitro fertilizability of bull spermatozoa. Anim. Reprod. Sci., 44: 23-31.
Khatir, H and Anouassi, A (2006). The first Dromedary (Camelus dromedarius) offspring obtained from in vitro matured, in vitro fertilized and in vitro cultured abattoir-derived oocytes. Theriogenology. 65: 1727-1736.
Khatir, H; Anouassi, A and Tibary, A (2007). Quality and developmental ability of Dromedary (Camelus dromeda-rius) embryos obtained by IVM/IVF, in vivo matured/IVF or in vivo matured/fertilized oocytes. Reprod. Dom. Anim., 42: 263-270.
Lambrechts, H; van Niekerk, FE; Coetzer, WA; Cloete, SWP and van der Horst, G (1999). The effect of cryopreservation on the survivability, viability and motility of epididymal African buffalo (Syncerus caffer) spermato-zoa. Theriogenology. 52: 1241-1249.
Martins, CF; Rumpf, R; Pereira, DC and Dode, MN (2007). Cryopreservation of epididymal bovine spermatozoa from dead animals and its uses in vitro embryo production. Anim. Reprod. Sci., 101: 326-331.
Moawad, AR; Darwish, GM; Badr, MR and El-Wishy, AB (2011). In vitro fertilization of Dromedary camel (Camelus dromedaries) oocytes with epididymal spermatozoa. Reprod. Fertil. Dev., 24: 192-193.
Monteiro, GA; Papa, FO; Zahn, FS; Dellaqua Jr, JA; Melo, CM; Maziero, RRD; Avanzi, BR; Alvarenga, MA and Guasti, PN (2011). Cryopreservation and fertility of ejaculated and epididymal stallion sperm. Anim. Reprod. Sci., 127: 197-201. Niasari-Naslaji, A; Mosaferi, S; Bahmani, N; Gerami, A; Gharahdaghi, AA; Abarghani, A and Ghanbari, A (2007). Semen cryopreservation in Bactrian camel (Camelus bactrianus) using Shotor diluent: effects of cooling rates and glycerol concentrations. Theriogenology. 68: 618-625. Niwa, K and Ohgoda, O (1988). Synergistic effect of caffeine and heparin on in vitro fertilization of cattle oocytes matured in culture. Theriogenology. 30: 733-741.
Nowshari, MA and Wani, NA (2005). Camelid embryo development in vitro: effect of protein supplementation in maturation medium and subsequent culture in two different media on fertilization and development. Reprod. Fertil. Dev., 17: 276-282.
Rath, D and Niemann, H (1997). In vitro fertilization of porcine oocytes with fresh and frozen-thawed ejaculated or frozen-thawed epididymal semen obtained from identical boars. Theriogenology. 47: 785-793.
Reyes-Moreno, C; Boilard, M; Sullivan, R and Sirard, M (2002). Characterization and identification of epididymal factors that protect ejaculated bovine sperm during in vitro storage. Biol. Reprod., 66: 159-166. Sieme, I; Merkt, H; Musa, B; Badreldin, H and Willmen, T (1990). Liquid and deep-freezing preservation of camel semenusing different extenders and methods. In: Proceedings of the workshop “Is it possible to improve the reproductive performance of the camel?”, Paris, September 10-12, 1990. PP: 273-284. Snedecor, GW and Cochran, WG (1989). Statistical methods. 8th Edn., Ames, IA, USA, Iowa State University Press. P: Turri, F; Kandil, OM; Abdoon, AS; Sabra, H; El Atrash, A and Pizzi, F (2013). Conservation of camel genetic resources: epididymal sperm recovery. The Camel Conference@ SOAS. 29 April 2013. PP: 27-32.
Turri, F; Madeddu, M; Gliozzi, TM; Gandini, G and Pizzi, F (2014). Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve gene banking in local breeds. Int. J. Anim. Biosci., 8: 440-447.
Varisli, O; Uguz, C; Agca, C and Agca, A (2009). Motility and acrosomal integrity comparisons between electro-ejaculated and epididymal ram sperm after exposure to a range of an isosmotic solutions, cryoprotective agents and low temperatures. Anim. Reprod. Sci., 110: 256-268. Wakayama, T and Yanagimachi, R (1998). Development of normal mice from oocytes injected with freeze-dried spermatozoa. Nat. Biotechnol., 16: 639-646. Wani, NA (2009). In vitro embryo production in camel (Camelus dromedarius) from in vitro matured oocytes fertilized with epididymal spermatozoa stored at 4°C. Anim. Reprod. Sci., 111: 69-79.
Wani, NA; Billah, M and Skidmore, JA (2008). Studies on liquefaction and storage of ejaculated Dromedary camel (Camelus dromedarius) semen. Anim. Reprod. Sci., 109: 309-318.
Wani, N; Nowshari, M and Wernery, U (2005). Storage of camel (Camelus dromedarius) epididymal spermatozoa in different extenders and at different temperatures. 38th Annu. Meet. Soc. Stud. Reprod., Quebec, Canada, W693.
Wani, NA and Wernery, U (2010). In vitro maturation of Dromedary (Camelus dromedarius) oocytes: effect of different protein supplementations and epidermal growth factor. Reprod. Dom. Anim., 45: e189-e193.
Yu, I and Leibo, SP (2002). Recovery of motile, membrane-intact spermatozoa from canine epididymides stored for 8 days at 4°C. Theriogenology. 57: 1179-1190.
Ziapour, S; Niasari-Naslaji, A; Mirtavousi, M; Keshavarz, M; Kalantari, A and Adel, H (2014). Semen collection using phantom in Dromedary. Anim. Reprod. Sci., 151: 15-21. | ||
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