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In silico prediction of B cell epitopes of the extracellular domain of insulin-like growth factor-1 receptor | ||
| Molecular Biology Research Communications | ||
| مقاله 1، دوره 5، شماره 4، اسفند 2016، صفحه 201-214 اصل مقاله (296.18 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2016.3832 | ||
| نویسندگان | ||
| Vahid Bayrami1؛ Mehrnaz Keyhanfar* 1؛ Hassan Mohabatkar1؛ Manijeh Mahdavi2؛ Violaine Moreau3 | ||
| 1Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran | ||
| 2Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 3Centre de Biochimie Structurale, CNRS UMR 5048, UM 1 & 2- INSERM U 1054, 29 rue de Navacelles, 34090, Montpellier, France | ||
| چکیده | ||
| The insulin-like growth factor-1 receptor (IGF-1R) is a transmembrane receptor with tyrosine kinase activity. The receptor plays a critical role in cancer. Using monoclonal antibodies (MAbs) against the IGF-1R, typically blocks ligand binding and enhances down-regulation of the cell-surface IGF-1R. Some MAbs such as cixutumumab are under clinical trial investigation. Targeting multiple distinct epitopes on IGF-1R, might be an effective strategy to inhibit IGF-1R pathway in cancer. In this study, new linear B cell epitopes for the extracellular domains of IGF-1R were predicted by in silico methods using a combination of linear B cell epitope prediction web servers such as ABCpred, Bepired, BCPREDs, Bcepred and Elliprro. Moreover, Discotope, B-pred and PEPOP web server tools were employed to predict new conformational B cell epitopes. In contrast to previously reported epitopes from extracellular region of the IGF-1R, we predicted new linear P8: (RQPQDGYLYRHNYCSK) and conformational Pc4: (HYYYAGVCVPACPPNTYRFE), Ppc6: (KMCPSTGKRENNESAPDNDT) and Ppc20: (ANILSAESSDSEFMQEPSGFI) epitopes. These epitopes are useful for further study as peptide antigens to actively immune host animals to develop new MAbs. Furthermore, the epitopes can be used in peptide-based cancer vaccines design. | ||
| کلیدواژهها | ||
| IGF-1R؛ Cancer therapy؛ B cell epitope؛ bioinformatics؛ Monoclonal antibody | ||
| مراجع | ||
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