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Estrogen receptor gene 1 expression in caprine and its effect on fertility | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 11، دوره 17، شماره 3 - شماره پیاپی 56، 2016، صفحه 207-209 اصل مقاله (199.58 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2016.3819 | ||
| نویسندگان | ||
| S. Saraswat* 1؛ P. K. Rout2؛ S. D. Kharche1؛ A. K. Goel1؛ S. K. Jindal1؛ S. Kumar1 | ||
| 1Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India | ||
| 2GGB Division, Central Institute for Research on goats (CIRG), Makhdoom, Farah- 281122, Mathura, Uttar Pradesh, India | ||
| چکیده | ||
| The present study was undertaken to analyze the expression pattern of estrogen receptor 1 gene (ESR1) in Barbari bucks (fertile and non-fertile) identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the spleen by Trizol method. The expression pattern of ESR1 gene was analyzed using real time polymerase chain reaction (RT-PCR). The expression pattern of ESR1 gene was analyzed by RT-PCR (Roche LC-480). Relative quantification by RT-PCR indicated that the ESR1 gene expression showed more fold in fertile bucks as compared to non-fertile. | ||
| کلیدواژه‌ها | ||
| ESR1 gene؛ Estrogen receptors؛ RNA؛ RT-PCR | ||
| مراجع | ||
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