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Genomic diversity of Clostridium perfringens strains isolated from food and human sources | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 2، دوره 17، شماره 3 - شماره پیاپی 56، 2016، صفحه 160-164 اصل مقاله (270.65 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2016.3809 | ||
| نویسندگان | ||
| A. Afshari1؛ A. Jamshidi* 2؛ J. Razmyar3؛ M. Rad4 | ||
| 1Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 2Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 3Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 4Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده | ||
| Clostridium perfringens is a serious pathogen which causes enteric diseases in domestic animals and food poisoning in humans. Spores can survive cooking processes and play an important role in the possible onset of disease. In this study, RAPD-PCR and REPPCR were used to examine the genetic diversity of 49 isolates of C. perfringens type A from three different sources. The results of RAPD-PCR revealed the most genetic diversity among poultry isolates, while human isolates showed the least genetic diversity. Cluster analysis obtained from RAPD-PCR and based on the genetic distances split the 49 strains into five distinct major clusters (A, B, C, D, and E). Cluster A and C were composed of isolates from poultry meat, cluster B was composed of isolates from human stool, cluster D was composed of isolates from minced meat, poultry meat and human stool and cluster E was composed of isolates from minced meat. Further characterization of these strains by using (GTG) 5 fingerprint repetitive sequence-based PCR analysis did not show further differentiation between various types of strains. In conclusion, RAPD-PCR method seems to be very promising for contamination source tracking in the field of food hygiene. | ||
| کلیدواژهها | ||
| Clostridium perfringens؛ Genetic diversity؛ RAPD-PCR؛ REP-PCR | ||
| مراجع | ||
|
Adak, GK; Long, SM and O’Brien, SJ (2002). Trends in indigenous foodborne disease and deaths, England and Wales: 1992 to 2000. Gut. 51: 832-841.
Afshari, A; Jamshidi, A; Razmyar, J and Rad, M (2015a). Genotyping of Clostridium perfringens isolated from broiler meat in Mashhad city, northeastern of Iran. Vet. Res. Forum., 6: 279-284.
Afshari, A; Jamshidi, A; Razmyar, J and Rad, M (2015b). Isolation and molecular typing of Clostridium perfringens isolated from minced meat. Iran. J. Vet. Sci. Technol., 7: 32-39.
Baker, A; Novak, K; Davis, E; Moser, R; Englehardt, M; Webel, D; Eldkamp, AV; Rehberger, T and Rosener, D (2006). Molecular analysis and diversity of pathogenic Clostridium perfringens and Clostridium difficile among neonatal pigs in the Midwest. Allen D. Leman Swine Conference, St. Paul, MN.
Barbut, F; Mario, N; Delmée, M; Gozian, J and Petit, JC (1993). Genomic fingerprinting of Clostridium difficile isolates by using a random amplified polymorphic DNA (RAPD) assay. FEMS. Microbiol. Lett., 114: 161-166.
Carman, RJ; Sayeed, S; Li, J; Genheimer, CW; Hiltonsmith, MF; Wilkins, TD and McClane, BA (2008). Clostridium perfringens toxin genotypes in the feces of healthy North Americans. Anaerobe. 14: 102-108.
Cilliers, FJ; Warren, RM; Hauman, JH; Wiid, IJF and Van Helden, PD (1997). Oligonucleotide (GTG) 5 as an epidemiological tool in the study of non tuberculous mycobacteria. J. Clin. Microbiol., 35: 1545-1549.
Gevers, D; Huys, G and Swings, J (2001). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS. Microbiol. Lett., 205: 31-36.
Gross, TP; Kamara, LB; Hatheway, CL; Powers, P; Libonati, JP; Harmon, SM and Israel, E (1989). Clostridium perfringens food poisoning: use of serotyping in an outbreak. J. Clin. Microbiol., 27: 660-663.
Heikinheimo, A; Lindström, M and Korkeala, H (2004). Enumeration and isolation of cpe-positive Clostridium perfringens spores from feces. J. Clin. Microbiol., 42: 3992-3997.
Jost, BH; Trinh, HT and Songer, JG (2006). Clonal relation-ships among Clostridium perfringens of porcine origin as determined by multilocus sequence typing. Vet. Microbiol., 116: 158-165.
Leflon-Guibout, V; Pons, JL; Heym, B and Nicolas-Chanoine, MH (1997). Typing of Clostridium perfringens strains by use of random amplified polymorphic DNA (RAPD) system in comparison with zymotyping. Anaerobe. 3: 245-250.
Lindström, M; Heikinheimo, A; Lahti, P and Korkeala, H (2011). Novel insights into the epidemiology of Clostri-dium perfringens type A food poisoning. Food Microbiol., 28: 192-198.
Ma, M; Li, J and McClane, BA (2012). Genotypic and phenotypic characterization of Clostridium perfringens isolates from Darmbrand cases in post-world war II Germany. Infect. Immun., 80: 4354-4363.
McClane, BA (2001). Clostridium perfringens. In: Doyle, MP; Beuchat, LR and Montville, TJ (Eds.), Food microbiology, fundamentals and frontiers. (2nd Edn.), Washington, ASM Press. PP: 351-372.
McLauchlin, J; Salmon, JE; Ahmed, S; Brazier, JS; Brett, MM; George, RC and Hood, J (2002). Amplified fragment length polymorphism (AFLP) analysis of Clostridium novyi, C. perfringens and Bacillus cereus isolated from injecting drug users during 2000. J. Med. Microbiol., 51: 990-1000.
Meer, R and Songer, JG (1997). Multiplex polymerase chain reaction assay for genotyping Clostridium perfringens. Am. J. Vet. Res., 58: 702-705.
Miwa, N; Nishina, T; Kubo, S and Honda, H (1997). Most probable numbers of enterotoxigenic C. perfringens in intestinal contents of domestic livestock detected by nested PCR. J. Vet. Med. Sci., 59: 557-560.
Moffatt, CR; Howard, PJ and Burns, T (2011). A mild outbreak of gastroenteritis in long-term care facility residents due to Clostridium perfringens, Australia 2009. Foodborne. Pathog. Dis., 8: 791-796.
Nakamura, H; Ogasawara, J; Monma, C; Hase, A; Suzuki, H; Kai, A; Haruki, K and Nishikawa, Y (2003). Useful-ness of a combination of pulsed-field gel electrophoresis and enrichment culture in laboratory investigation of a foodborne outbreak due to Clostridium perfringens. Diagn. Microbiol. Infect. Dis., 47: 471-475.
Northey, G; Gal, M; Rahmati, A and Brazier, JS (2005). Subtyping of Clostridium difficile PCR ribotype 001 by REP-PCR and PFGE. J. Med. Microbiol., 54: 543-547.
Novak, JS and Juneja, VK (2002). Clostridium perfringens: hazards in new generation foods. Innov. Food. Sci. Emerg. Technol., 3: 127-132.
Power, EG (1996). RAPD typing in microbiology- a technical review. J. Hosp. Infect., 34: 247-265.
Schalch, B; Bader, L; Schau, HP; Bergmann, R; Rometsch, A; Maydl, G and Kessler, S (2003). Molecular typing of Clostridium perfringens from a food-borne disease out-break in a nursing home: ribotyping versus pulsed-field gel electrophoresis. J. Clin. Microbiol., 41: 892-895.
Schalch, B; Eisgruber, H; Schau, HP; Wiedmann, M and Stolle, A (1998). Strain differentiation of Clostridium perfringens by bacteriocin typing, plasmid profiling and ribotyping. Zbl. Vet. Med. B., 45: 595-602. Schalch, B; Sperner, B; Eisgruber, H and Stolle, A (1999). Molecular methods for the analysis of Clostridium perfringens relevant to food hygiene. FEMS. Immunol. Med. Microbiol., 24: 281-286.
Settings, M (2006). AFLP fingerprinting of Colombian Clostridium spp strains, multivariate data analysis and its taxonomical implications. J. Microbiol. Meth., 67: 64-69.
Siragusa, G; Danyluk, M; Hiett, K; Wise, M and Craven, S (2006). Molecular subtyping of poultry-associated type A Clostridium perfringens isolates by repetitive-element PCR. J. Clin. Microbiol., 44: 1065-1073.
Svec, P; Vancanneyt, M; Seman, M; Snauwaert, C; Lefebvre, K; Sedláček, I and Swings, J (2005). Evaluation of (GTG) 5-PCR for identification of Entero-coccus spp. FEMS. Microbiol. Lett., 247: 59-63.
Uzal, FA; Vidal, JE; McClane, BA and Gurjar, AA (2010). Clostridium perfringens toxins involved in mammalian veterinary diseases. Open. Toxinol. J., 2: 24-42.
Van Asten, AJ; Nikolaou, G and Gröne, A (2010). The occurrence of cpb2-toxigenic Clostridium perfringens and the possible role of the b2-toxin in enteric disease of domestic animals, wild animals and humans. Vet. J., 183: 135-140.
Versalovic, J; Schneider, M; De Bruijn, FJ and Lupski, JR (1994). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol. Cell. Biol., 5: 25-40.
Wahl, E; Rømma, S and Granum, PE (2012). A Clostridium perfringens outbreak traced to temperature-abused beef stew, Norway, 2012. Public Health
Wen, Q and McClane, BA (2004). Detection of entero-toxigenic Clostridium perfringens type A isolates in American retail foods. Appl. Environ. Microbiol., 70: 2685-2691.
Williams, JGK; Kubelik, AR; Livak, KJ; Rafalski, JA and Tingey, SV (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 6531-6535.
Wu, J; Zhang, W; Xie, B; Wu, M; Tong, X; Kalpoe, J and Zhang, D (2009). Detection and toxin typing of Clostri-dium perfringens in formalin-fixed, paraffin-embedded tissue samples by PCR. J. Clin. Microbiol., 47: 807-810.
Xiao, Y; Wagendorp, A; Moezelaar, R; Abee, T and Wells-Bennik, MH (2012). A wide variety of Clostridium perfringens type A food-borne isolates that carry a chromosomal cpe gene belong to one multilocus sequence typing cluster. Appl. Environ. Microbiol., 78: 7060-7068.
Yan, WK (1989). Use of host modified bacteriophages in development of a phage typing scheme for Clostridium perfringens. Med. Lab. Sci., 46: 186-193. | ||
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