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BIBI, YAMIN, NAEEM, JAVERIA, ZAHARA, KULSOOM, ARSHAD, MUHAMMAD, QAYYUM, ABDUL. (1394). IN VITRO ANTIMICROBIAL ASSESSMENT OF SELECTED PLANT EXTRACTS FROM PAKISTAN. , (), -. doi: 10.22099/ijsts.2016.3532
YAMIN BIBI; JAVERIA NAEEM; KULSOOM ZAHARA; MUHAMMAD ARSHAD; ABDUL QAYYUM. "IN VITRO ANTIMICROBIAL ASSESSMENT OF SELECTED PLANT EXTRACTS FROM PAKISTAN". , , , 1394, -. doi: 10.22099/ijsts.2016.3532
BIBI, YAMIN, NAEEM, JAVERIA, ZAHARA, KULSOOM, ARSHAD, MUHAMMAD, QAYYUM, ABDUL. (1394). 'IN VITRO ANTIMICROBIAL ASSESSMENT OF SELECTED PLANT EXTRACTS FROM PAKISTAN', , (), pp. -. doi: 10.22099/ijsts.2016.3532
BIBI, YAMIN, NAEEM, JAVERIA, ZAHARA, KULSOOM, ARSHAD, MUHAMMAD, QAYYUM, ABDUL. IN VITRO ANTIMICROBIAL ASSESSMENT OF SELECTED PLANT EXTRACTS FROM PAKISTAN. , 1394; (): -. doi: 10.22099/ijsts.2016.3532

IN VITRO ANTIMICROBIAL ASSESSMENT OF SELECTED PLANT EXTRACTS FROM PAKISTAN

Iranian Journal of Science
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 26 دی 1394
نوع مقاله: Regular Paper
شناسه دیجیتال (DOI): 10.22099/ijsts.2016.3532
نویسندگان
YAMIN BIBI1؛ JAVERIA NAEEM1؛ KULSOOM ZAHARA1؛ MUHAMMAD ARSHAD1؛ ABDUL QAYYUM* 2
1Department of Botany, Pir Mehr Ali Shah, Arid Agriculture University Rawalpindi.
2Department of Agricultural Sciences, University of Haripur
چکیده
Screening of ethno medicinal plants is a pre-requisite for discovery of new drug components. Polar as well as nonpolar extracts from four important plants (Cedrela serrata, Hedera helix, Hedera nepalensis and Cuscuta reflexa) were tested against two gram positive (Staphylococcus epidermidis, Staphylococcus aureus) and three gram negative (Escherichia coli, Pseudomonas sp. and Klebsiella pneumoniae) bacterial strains as well as two pathogenic fungal strains (Fusarium oxysporum and Penicillium sp.). Bacterial and fungal pathogens were tested by using agar well diffusion and agar tube dilutions methods respectively. Methanol extracts from all selected plant species showed activity against all tested bacterial strains. Maximum inhibition was shown by Cuscuta reflexa methanol extract (19.2 mm) against Klebsiella pneumoniae. Inhibition ranges observed for other methanol extracts were 12-15 mm, 12-16 mm and 9-16 mm in Hedera nepalensis, Hedera helix and Cedrela serrata respectively. Chloroform extract of Cuscuta reflexa inhibited all bacterial strains with maximum inhibition zone of 21mm against Klebsiella pneumoniae. Chloroform extract from other plant species proved inactive against all bacterial strains except Escherichia coli and Pseudomonas sp. In the case of fungal strains Fusarium oxysporum was inhibited maximum (78%) by chloroform extract of Hedera helix, however, other extracts showed moderate or low activity. In conclusion active extracts of present study can be suitable candidates for new drug discovery in future.
کلیدواژه‌ها
Cedrela؛ Maceration؛ Escherichia coli؛ Extract؛ Tube Dilution؛ Antimicrobial
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