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Farooq, Umer, Latif, A., Irshad, H., Ullah, A., Zahur, A. B., Naeem, K., Khan, S. U. H., Ahmed, Z., Rodriguez, L. L., Smoliga, G.. (1394). Loop-mediated isothermal amplification (RT-LAMP): a new approach for the detection of foot-and-mouth disease virus and its sero-types in Pakistan. , 16(4), 331-334. doi: 10.22099/ijvr.2015.3283
Umer Farooq; A. Latif; H. Irshad; A. Ullah; A. B. Zahur; K. Naeem; S. U. H. Khan; Z. Ahmed; L. L. Rodriguez; G. Smoliga. "Loop-mediated isothermal amplification (RT-LAMP): a new approach for the detection of foot-and-mouth disease virus and its sero-types in Pakistan". , 16, 4, 1394, 331-334. doi: 10.22099/ijvr.2015.3283
Farooq, Umer, Latif, A., Irshad, H., Ullah, A., Zahur, A. B., Naeem, K., Khan, S. U. H., Ahmed, Z., Rodriguez, L. L., Smoliga, G.. (1394). 'Loop-mediated isothermal amplification (RT-LAMP): a new approach for the detection of foot-and-mouth disease virus and its sero-types in Pakistan', , 16(4), pp. 331-334. doi: 10.22099/ijvr.2015.3283
Farooq, Umer, Latif, A., Irshad, H., Ullah, A., Zahur, A. B., Naeem, K., Khan, S. U. H., Ahmed, Z., Rodriguez, L. L., Smoliga, G.. Loop-mediated isothermal amplification (RT-LAMP): a new approach for the detection of foot-and-mouth disease virus and its sero-types in Pakistan. , 1394; 16(4): 331-334. doi: 10.22099/ijvr.2015.3283

Loop-mediated isothermal amplification (RT-LAMP): a new approach for the detection of foot-and-mouth disease virus and its sero-types in Pakistan

Iranian Journal of Veterinary Research
مقاله 1، دوره 16، شماره 4 - شماره پیاپی 53، 2015، صفحه 331-334    اصل مقاله (61.71 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2015.3283
نویسندگان
Umer Farooq* 1؛ A. Latif2؛ H. Irshad2؛ A. Ullah2؛ A. B. Zahur2؛ K. Naeem2؛ S. U. H. Khan2؛ Z. Ahmed3؛ L. L. Rodriguez3؛ G. Smoliga3
1National Agricultural Research Center
2Animal Health Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, 45500, Pakistan
3Plum Island Animal Disease Centre, Foreign Animal Disease Research Unit, New York 11944, USA
چکیده
Successful disease management requires a rapid and sensitive diagnosis method that can recognize early infection even before the manifestation of its clinical signs. The only available field diagnostic tests for foot-and-mouth disease (FMD) are lateral flow devices, commonly known as chromatographic strips. Low sensitivity and inability to detect FMD virus (FMDV) at the serotype level are limitations of lateral flow devices. Therefore, a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) was standardized using universal and sero-type specific genes in a single tube. This test does not require sophisticated equipment and can detect FMDV at serotype level in about 60 min. In addition, the sensitivity and specificity of this test is comparable to conventional reverse transcriptase PCR and real time PCR (rRT-PCR).
کلیدواژه‌ها
FMD؛ RT-LAMP؛ Sensitivity
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