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Ghoddusi, A., Nayeri Fasaei, B., Karimi, V., Ashrafi Tamai, I., Moulana, Z., Zahraei Salehi, T.. (1394). Molecular identification of Salmonella Infantis isolated from backyard chickens and detection of their resistance genes by PCR. , 16(3), 293-297. doi: 10.22099/ijvr.2015.3198
A. Ghoddusi; B. Nayeri Fasaei; V. Karimi; I. Ashrafi Tamai; Z. Moulana; T. Zahraei Salehi. "Molecular identification of Salmonella Infantis isolated from backyard chickens and detection of their resistance genes by PCR". , 16, 3, 1394, 293-297. doi: 10.22099/ijvr.2015.3198
Ghoddusi, A., Nayeri Fasaei, B., Karimi, V., Ashrafi Tamai, I., Moulana, Z., Zahraei Salehi, T.. (1394). 'Molecular identification of Salmonella Infantis isolated from backyard chickens and detection of their resistance genes by PCR', , 16(3), pp. 293-297. doi: 10.22099/ijvr.2015.3198
Ghoddusi, A., Nayeri Fasaei, B., Karimi, V., Ashrafi Tamai, I., Moulana, Z., Zahraei Salehi, T.. Molecular identification of Salmonella Infantis isolated from backyard chickens and detection of their resistance genes by PCR. , 1394; 16(3): 293-297. doi: 10.22099/ijvr.2015.3198

Molecular identification of Salmonella Infantis isolated from backyard chickens and detection of their resistance genes by PCR

Iranian Journal of Veterinary Research
مقاله 10، دوره 16، شماره 3 - شماره پیاپی 52، 2015، صفحه 293-297    اصل مقاله (276.63 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2015.3198
نویسندگان
A. Ghoddusi1؛ B. Nayeri Fasaei* 2؛ V. Karimi3؛ I. Ashrafi Tamai4؛ Z. Moulana5؛ T. Zahraei Salehi2
1Ph.D. Student in Bacteriology, Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4Ph.D. Student in Bacteriology, Department of Microbiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
5Department of Laboratory and Environment Health, Faculty of Paramedical Science, Babol University of Medical Sciences, Babol, Iran
چکیده
This study aims at molecular identification of Salmonella Infantis isolated from backyard chickens and the detection of their antibiotic resistance genes. A total of 46 Salmonella-suspected samples isolated from backyard chickens of northern Iran were collected. Serotyping was done by the traditional method and then confirmed by PCR. Antimicrobial susceptibility of the isolates against 13 antimicrobial agents was determined by the standard disk diffusion method. There were 44 samples identified as Salmonella. Serotyping results showed that all 44 isolates belonged to serogroup C1 and serovar Infantis. The most resistance observed was to tetracycline and doxycycline (100%), chloramphenicol (79%) and florfenicol (72%). The floR, catI, tetA and tetG genes were used for the detection of florfenicol chloramphenicol and tetracycline resistance. In order to identify the phenotypic resistance in strains which showed resistance genes by PCR, colony PCR and culture on plates each containing antibiotic was performed simultaneously. All the Salmonella Infantis resistant to florfenicol and chloramphenicol harbored floR and catI. None of the Salmonella resistant to tetracycline carried tetA or tetG. The result of colony PCR and culture in antibiotic medium confirmed the
results of PCR and indicated phenotypic resistance in these samples.
کلیدواژه‌ها
Backyard chickens؛ Resistance genes؛ Salmonella Infantis
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