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Abdolahnejad, Zeinab, Pourkazemi, Mohammad, Khoshkholgh, Majid Reza, Yarmohammadi, Mahtab. (1394). Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii). , 4(4), 181-188. doi: 10.22099/mbrc.2015.3166
Zeinab Abdolahnejad; Mohammad Pourkazemi; Majid Reza Khoshkholgh; Mahtab Yarmohammadi. "Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)". , 4, 4, 1394, 181-188. doi: 10.22099/mbrc.2015.3166
Abdolahnejad, Zeinab, Pourkazemi, Mohammad, Khoshkholgh, Majid Reza, Yarmohammadi, Mahtab. (1394). 'Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)', , 4(4), pp. 181-188. doi: 10.22099/mbrc.2015.3166
Abdolahnejad, Zeinab, Pourkazemi, Mohammad, Khoshkholgh, Majid Reza, Yarmohammadi, Mahtab. Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii). , 1394; 4(4): 181-188. doi: 10.22099/mbrc.2015.3166

Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)

Molecular Biology Research Communications
مقاله 2، دوره 4، شماره 4، اسفند 2015، صفحه 181-188    اصل مقاله (182.9 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2015.3166
نویسندگان
Zeinab Abdolahnejad* 1؛ Mohammad Pourkazemi2؛ Majid Reza Khoshkholgh1؛ Mahtab Yarmohammadi3
1Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara-Iran.
2Iran Fishery Research Organization (IFRO), Tehran, Iran.
3International Sturgeon Research Institute, Rasht-Iran.
چکیده
The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRNA expression. The results showed that the GH mRNA could be observed in the eyed eggs and even at unfertilized eggs of Siberian sturgeon. The highest amounts of GH mRNA were found at 25 and 50 dph larvae, while the lowest levels were detected at 1 and 3 dph larvae stage. These findings suggest that, the GH mRNA play a key role during developmental stages of Siberian sturgeon. 
کلیدواژه‌ها
Siberian sturgeon؛ Growth hormone؛ mRNA expression؛ RT-PCR
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