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Assessment of the vacuolar Na+/H+ antiporter (NHX1) transcriptional changes in Leptochloa fusca L. in response to salt and cadmium stresses | ||
| Molecular Biology Research Communications | ||
| مقاله 1، دوره 4، شماره 3، آذر 2015، صفحه 133-142 اصل مقاله (310.97 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2015.3115 | ||
| نویسندگان | ||
| Hamed Adabnejad1؛ Hamid Reza Kavousi* 2؛ Hadi Hamidi3؛ Iraj Tavassolian4 | ||
| 1Department of biotechnology, faculty of agriculture, shahid bahonar university of kerman, kerman, Iran | ||
| 2Department of biotechnology, college of agricult, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| 3Department of biotechnology, faculty of agriculture, shahid bahonar university of Kerman, Kerman, Iran | ||
| 4Department of horticulture, faculty of agriculture, shahid bahonar university of kerman, kerman, Iran | ||
| چکیده | ||
| Sodium/proton exchangers (NHX) are key players in plant responses to salinity and have a central role in establishing ion homeostasis. NHXs can be localized in tonoplast or plasma membranes, where they exchange sodium ions for protons, resulting in the removal of ions from the cytosol into vacuole or extracellular spaces. In the present study, the expression pattern of the gene encoding Na+/H+ antiporter in the vacuolar membrane (NHX1 gene) in Leptochloa fusca (Kallar grass) was measured by a semi-quantitative RT-PCR method under different treatments of NaCl and CdCl2. Results indicated that NaCl positively affected expression levels of LfNHX1, and that the amount of LfNHX1 mRNA increased in conjunction with the rise of salinity pressure, This finding suggests that vacuolar Na+/H+ antiporter might play an important role in the salt tolerance ability of kallar grass. The results also showed that cadmium exposure significantly modulated the mRNA expression of the LfNHX1 gene, suggesting that cadmium exposure disturbed Na+ homeostasis across the tonoplast and decreased the salt tolerance ability of kallar grass. | ||
| کلیدواژهها | ||
| Kallar grass؛ Salt stress؛ cadmium؛ NHX1؛ Semi-quantitative RT-PCR | ||
| مراجع | ||
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