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Awad, A., Khalil, S. R., Abd-Elhakim, Y. M.. (1394). Molecular phylogeny of some avian species using Cytochrome b gene sequence analysis. , 16(2), 218-222. doi: 10.22099/ijvr.2015.3072
A. Awad; S. R. Khalil; Y. M. Abd-Elhakim. "Molecular phylogeny of some avian species using Cytochrome b gene sequence analysis". , 16, 2, 1394, 218-222. doi: 10.22099/ijvr.2015.3072
Awad, A., Khalil, S. R., Abd-Elhakim, Y. M.. (1394). 'Molecular phylogeny of some avian species using Cytochrome b gene sequence analysis', , 16(2), pp. 218-222. doi: 10.22099/ijvr.2015.3072
Awad, A., Khalil, S. R., Abd-Elhakim, Y. M.. Molecular phylogeny of some avian species using Cytochrome b gene sequence analysis. , 1394; 16(2): 218-222. doi: 10.22099/ijvr.2015.3072

Molecular phylogeny of some avian species using Cytochrome b gene sequence analysis

Iranian Journal of Veterinary Research
مقاله 16، دوره 16، شماره 2 - شماره پیاپی 51، 2015، صفحه 218-222    اصل مقاله (466.11 K)
نوع مقاله: Short paper
شناسه دیجیتال (DOI): 10.22099/ijvr.2015.3072
نویسندگان
A. Awad* 1؛ S. R. Khalil2؛ Y. M. Abd-Elhakim2
1Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, 44511 Zagazig, Egypt
2Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, 44511 Zagazig, Egypt
چکیده
Veritable identification and differentiation of avian species is a vital step in conservative, taxonomic, forensic, legal and other ornithological interventions. Therefore, this study involved the application of molecular approach to identify some avian species i.e. Chicken (Gallus gallus), Muskovy duck (Cairina moschata), Japanese quail (Coturnix japonica), Laughing dove (Streptopelia senegalensis), and Rock pigeon (Columba livia). Genomic DNA was extracted from blood samples and partial sequence of the
mitochondrial cytochrome b gene (358 bp) was amplified and sequenced using universal primers. Sequences alignment and phylogenetic analyses were performed by CLC main workbench program. The obtained five sequences were deposited in GenBank and compared with those previously registered in GenBank. The similarity percentage was 88.60% between Gallus gallus and Coturnix japonica and 80.46% between Gallus gallus and Columba livia. The percentage of identity between the studied species and GenBank species ranged from 77.20% (Columba oenas and Anas platyrhynchos) to 100% (Gallus gallus and Gallus sonneratii, Coturnix coturnix and Coturnix japonica, Meleagris gallopavo and Columba livia). Amplification of the partial sequence of mitochondrial cytochrome b gene proved to be practical for identification of an avian species unambiguously.
کلیدواژه‌ها
Avian species؛ Cytochrome b gene؛ Phylogenetic analysis
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