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Abedi, V., Razmi, Gh., Seifi, H., Naghibi, A.. (1394). Molecular detection of equine piroplasms in donkeys (Equus asinus) in North Khorasan province, Iran. , 16(2), 202-204. doi: 10.22099/ijvr.2015.3068
V. Abedi; Gh. Razmi; H. Seifi; A. Naghibi. "Molecular detection of equine piroplasms in donkeys (Equus asinus) in North Khorasan province, Iran". , 16, 2, 1394, 202-204. doi: 10.22099/ijvr.2015.3068
Abedi, V., Razmi, Gh., Seifi, H., Naghibi, A.. (1394). 'Molecular detection of equine piroplasms in donkeys (Equus asinus) in North Khorasan province, Iran', , 16(2), pp. 202-204. doi: 10.22099/ijvr.2015.3068
Abedi, V., Razmi, Gh., Seifi, H., Naghibi, A.. Molecular detection of equine piroplasms in donkeys (Equus asinus) in North Khorasan province, Iran. , 1394; 16(2): 202-204. doi: 10.22099/ijvr.2015.3068

Molecular detection of equine piroplasms in donkeys (Equus asinus) in North Khorasan province, Iran

Iranian Journal of Veterinary Research
مقاله 12، دوره 16، شماره 2 - شماره پیاپی 51، 2015، صفحه 202-204    اصل مقاله (186.51 K)
نوع مقاله: Short paper
شناسه دیجیتال (DOI): 10.22099/ijvr.2015.3068
نویسندگان
V. Abedi1؛ Gh. Razmi* 1؛ H. Seifi2؛ A. Naghibi1
1Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده
Equine piroplasmosis is a tickborne disease of equids with worldwide distribution, caused by Theileria equi and Babesia caballi. The aim of this study was molecular detection of T. equi and B. caballi in donkeys in northeastern Iran and investigate the association between positivity of piroplasm infection and host-related factors. In the present study, Blood samples were collected from 106 apparently healthy donkeys (Equus asinus) in North Khorasan province, Iran. Blood smears were prepared and stained by giemsa method. DNA was extracted from blood and then multiplex-PCR was done for detection of any piroplasms infection. According to the results, four donkeys showed T. equi in blood smears but B. caballi was not found. Also, fifty four donkeys (50.94%) showed T. equi infection using multiplex-PCR. No siginificant difference was observed between the frequency of T. equi infection with hostrelated factors in donkeys. This is the first report on the molecular detection of eqiune piroplamosis in donkeys in Iran. Also, no significant association was found between the rate of T. equi infected animals.
کلیدواژه‌ها
Babesia caballi؛ Donkey؛ PCR؛ Theileria equi
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