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Mahmoud, K. Gh. M., El-Sokary, A. A. E., Abdel-Ghaffar, A. E., Abou El-Roos, M. E. A., Ahmed, Y. F.. (1394). Analysis of chromatin integrity and DNA damage of buffalo spermatozoa. , 16(2), 161-166. doi: 10.22099/ijvr.2015.3042
K. Gh. M. Mahmoud; A. A. E. El-Sokary; A. E. Abdel-Ghaffar; M. E. A. Abou El-Roos; Y. F. Ahmed. "Analysis of chromatin integrity and DNA damage of buffalo spermatozoa". , 16, 2, 1394, 161-166. doi: 10.22099/ijvr.2015.3042
Mahmoud, K. Gh. M., El-Sokary, A. A. E., Abdel-Ghaffar, A. E., Abou El-Roos, M. E. A., Ahmed, Y. F.. (1394). 'Analysis of chromatin integrity and DNA damage of buffalo spermatozoa', , 16(2), pp. 161-166. doi: 10.22099/ijvr.2015.3042
Mahmoud, K. Gh. M., El-Sokary, A. A. E., Abdel-Ghaffar, A. E., Abou El-Roos, M. E. A., Ahmed, Y. F.. Analysis of chromatin integrity and DNA damage of buffalo spermatozoa. , 1394; 16(2): 161-166. doi: 10.22099/ijvr.2015.3042

Analysis of chromatin integrity and DNA damage of buffalo spermatozoa

Iranian Journal of Veterinary Research
مقاله 5، دوره 16، شماره 2 - شماره پیاپی 51، 2015، صفحه 161-166    اصل مقاله (263.91 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2015.3042
نویسندگان
K. Gh. M. Mahmoud* 1؛ A. A. E. El-Sokary2؛ A. E. Abdel-Ghaffar3؛ M. E. A. Abou El-Roos3؛ Y. F. Ahmed1
1Department of Animal Reproduction and Artificial Insemination, National Research Center, Dokki, Giza, Egypt
2General Organization for Veterinary Services, Dokki, Giza, Egypt
3Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt
چکیده
This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly significant differences (P<0.001) in chromatin integrity were observed between fresh and frozen semen. For the fresh semen, there was no significant difference between the bulls for chromatin integrity; however, a significant variation (P<0.05) was detected in their frozen semen. No DNA fragmentation was observed by agarose gel electrophoresis. The percentage of sperm with damaged DNA detected by comet assay differed significantly between fresh and frozen semen. A significant negative correlation was recorded between motility and DNA damage (r=-0.68, P<0.05). Sperm abnormalities and DNA fragmentation were significantly positively correlated (r=0.59, P<0.05). In conclusion, DNA damage evaluation can provide reassurance about genomic normalcy and guide the development of improved methods of selecting spermatozoa with intact DNA to
be used in artificial insemination.
کلیدواژه‌ها
Buffalo bull؛ Chromatin integrity؛ DNA damage؛ Semen quality
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