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Ali Nasir, A., Younus, Younus, M., Rashid, A., Abdul Khaliq, S., Khan, E., Shah, S. H., Aslam, A., Ghumman, M. A., Joiya, M. H.. (1393). Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes. , 16(1), 94-99. doi: 10.22099/ijvr.2015.2931
A. Ali Nasir; Younus, M. Younus; A. Rashid; S. Abdul Khaliq; E. Khan; S. H. Shah; A. Aslam; M. A. Ghumman; M. H. Joiya. "Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes". , 16, 1, 1393, 94-99. doi: 10.22099/ijvr.2015.2931
Ali Nasir, A., Younus, Younus, M., Rashid, A., Abdul Khaliq, S., Khan, E., Shah, S. H., Aslam, A., Ghumman, M. A., Joiya, M. H.. (1393). 'Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes', , 16(1), pp. 94-99. doi: 10.22099/ijvr.2015.2931
Ali Nasir, A., Younus, Younus, M., Rashid, A., Abdul Khaliq, S., Khan, E., Shah, S. H., Aslam, A., Ghumman, M. A., Joiya, M. H.. Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes. , 1393; 16(1): 94-99. doi: 10.22099/ijvr.2015.2931

Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes

Iranian Journal of Veterinary Research
مقاله 16، دوره 16، شماره 1 - شماره پیاپی 50، 2015، صفحه 94-99    اصل مقاله (430.17 K)
نوع مقاله: Short paper
شناسه دیجیتال (DOI): 10.22099/ijvr.2015.2931
نویسندگان
A. Ali Nasir* 1؛ Younus, M. Younus2؛ A. Rashid1؛ S. Abdul Khaliq1؛ E. Khan2؛ S. H. Shah1؛ A. Aslam3؛ M. A. Ghumman1؛ M. H. Joiya1
1Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan
2Department of Pathology, College of Veterinary Science, Jhang Campus, Jhang, Pakistan
3Department of Media Sterilization, University of Veterinary and Animal Sciences, Lahore, Pakistan
چکیده
The present investigation was conducted to study the effects of experimental Clostridium perfringens type D enterotoxaemia in teddy goats. Clinical signs started to appear after 30 min of experimental infection like anorexia, diarrhea, dehydration, frothing and dyspnea. Gross lesions consisted of severe congestion in tissues of varying intensity with enlarged mesenteric lymph nodes while histological examination revealed edema of lungs, kidney, and lymph nodes and to some extent in brain along with hemorrhages in
lungs and intestines. Clostridium perfringens type D carrying alpha and epsilon toxin genes were amplified with amplicon size about 247 bp and 665 bp, respectively. Human erythrocytes showed the highest hemolysis, 68%, followed by mice, 57%, against culture supernatants. The percentage of hemolysis was significantly higher at 37°C as compared to 25°C except for rabbit and dog.
کلیدواژه‌ها
Clostridium perfringens type D؛ Enterotoxaemia ELISA؛ Alpha and Epsilon genes؛ Hemolysis
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