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Evaluation of humoral immunity and protective efficacy of biofilm producing Staphylococcus aureus bacterin-toxoid prepared from a bovine mastitis isolate in rabbit | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 11، دوره 16، شماره 1 - شماره پیاپی 50، 2015، صفحه 69-74 اصل مقاله (260.96 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2015.2927 | ||
| نویسندگان | ||
| A. Raza* 1؛ G. Muhammad1؛ S. U. Rahman2؛ I. Rashid1؛ K. Hanif3؛ A. Atta4؛ S. Sharif4 | ||
| 1Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan | ||
| 2Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan | ||
| 3Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan | ||
| 4Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan | ||
| چکیده | ||
| Mastitis is a one of the major diseases of dairy animals. Staphylococcus aureus is the most common microorganism associated with this dairy scourge. Cure rates of mastitis associated with this pathogen are appallingly low. Biofilm is an important virulence factor and immunogenic structure of S. aureus that makes it resistant to phagocytosis and antibiotics. Reports on the efficacy of vaccine prepared from a biofilm producing S. aureus are infrequent. The present study was designed to evaluate the role of a bacterin-toxoid prepared from a strong biofilm producing S. aureus in effective immunization of rabbits. The strong biofilm producing S. aureus selected from 64 isolates of staphylococci was used to prepare bacterin-toxoid and aluminum hydroxide gel was added as an adjuvant. The vaccine was evaluated in rabbits by challenge protection assay and humoral immune response. The mortality rates in control and vaccinated groups were 80% and 10% at day 7 post challenge and 100% and 20% at day 15 post challenge, respectively. Serum antibody titer (GMT) was significantly higher (294.0) in vaccinated group as compared to control group of rabbits (2.63) at day 45. The results showed that the vaccine has significantly elicited humoral immune response in rabbit and developed protective efficacy against new infections. | ||
| کلیدواژهها | ||
| Mastitis؛ Staphylococcus aureus؛ Biofilm production؛ Immune response؛ Rabbit | ||
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