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Salehi, Abdolreza, Sobhani, Rohallah, Aminafshar, Mahdi, Sayyadnejhad, Mohammad, Nasiri, Khadijeh. (1393). Single nucleotide polymorphism of FGF2 gene in Iranian Holstein proven bulls. , 4(1), 57-62. doi: 10.22099/mbrc.2015.2916
Abdolreza Salehi; Rohallah Sobhani; Mahdi Aminafshar; Mohammad Sayyadnejhad; Khadijeh Nasiri. "Single nucleotide polymorphism of FGF2 gene in Iranian Holstein proven bulls". , 4, 1, 1393, 57-62. doi: 10.22099/mbrc.2015.2916
Salehi, Abdolreza, Sobhani, Rohallah, Aminafshar, Mahdi, Sayyadnejhad, Mohammad, Nasiri, Khadijeh. (1393). 'Single nucleotide polymorphism of FGF2 gene in Iranian Holstein proven bulls', , 4(1), pp. 57-62. doi: 10.22099/mbrc.2015.2916
Salehi, Abdolreza, Sobhani, Rohallah, Aminafshar, Mahdi, Sayyadnejhad, Mohammad, Nasiri, Khadijeh. Single nucleotide polymorphism of FGF2 gene in Iranian Holstein proven bulls. , 1393; 4(1): 57-62. doi: 10.22099/mbrc.2015.2916

Single nucleotide polymorphism of FGF2 gene in Iranian Holstein proven bulls

Molecular Biology Research Communications
مقاله 6، دوره 4، شماره 1، خرداد 2015، صفحه 57-62    اصل مقاله (277.67 K)
نوع مقاله: Original article
شناسه دیجیتال (DOI): 10.22099/mbrc.2015.2916
نویسندگان
Abdolreza Salehi* 1؛ Rohallah Sobhani2؛ Mahdi Aminafshar3؛ Mohammad Sayyadnejhad4؛ Khadijeh Nasiri2
1Department of Animal and Poultry Science, College of Abouraihan, University of Tehran, Iran
2Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Tehran, Pakdasht, Islamic Republic of Iran.
3Department of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran
4Animal Breeding Center of Iran, Karaj, Islamic Republic of Iran
چکیده
Fibroblast growth factor 2 (FGF2) serves in the uterine endometrium during estrous presenting in the bovine mammary gland which is responsible to express interferon-T (IFNT), and is an important agent to encourage the continuation of pregnancy in the ruminants. Significant associations have been found between genes affected by IFNT and genes that are responsible for milk production traits. Semen samples from 101 Iranian Holstein proven bulls were collected to extract the genomic DNA. Forward and reverse primers were designed and a 710-base-pair fragment in intron 1 was amplified using PCR technique. To detect single nucleotide polymorphism (SNP), all samples were sequenced. Three positions including 11474 (C/G), 11513 (C/G) and 11646 (A/G) were considered. The 11474C, 11513C and 11646A alleles are known as wild type alleles. In this study all animals were distinguished as the11474C, 11513C and 11646A alleles. Furthermore, amplified fragments were under consideration to detect new SNPs. Only one new SNP in one sample was observed at position 11863 resulting substitution of thymine to cytosine. This new mutation has been registered on the NCBI database with accession number HM597774.
کلیدواژه‌ها
FGF2؛ SNP؛ Iranian Holstein؛ Polymorphism؛ Candidate gene
مراجع
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