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DETERMINATION OF PLOIDY LEVELS OF SOME POPULATIONS OF AGROPYRON CRISTATUM (POACEAE) IN IRAN BY FLOW CYTOMETRY | ||
| Iranian Journal of Science | ||
| مقاله 15، دوره 28، شماره 1، شهریور 2004، صفحه 137-144 اصل مقاله (330.02 K) | ||
| نوع مقاله: Regular Paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijsts.2004.2842 | ||
| نویسندگان | ||
| M. YOUSOFI* 1؛ A. ARYAVAND2 | ||
| 1Department of Biology, Universities of Isfahan and Payam Noor, Isfahan, I. R of Iran | ||
| 2Department of Biology, University of Isfahan, Isfahan, I. R of Iran | ||
| چکیده | ||
| Flow cytometry (FCM) was used to determine the ploidy levels of six different populations of Agropyron cristatum (L.) Gaertn. (Poaceae) in Iran, using a diploid cultivated barley (Hordeum vulgare L.) as an internal reference. According to flow cytometric data, tetraploid (2n = ± 28) and hexaploid (2n = ± 42) levels were detected among the examined populations and these results were supported by chromosome counting on the same materials included in this study. The mean nuclear DNA content (2C value) of the populations were then estimated, ranging from 26.41 to 27.56 pg for two varieties of Agropyron cristatum subsp. pectinatum, and 43.47 pg for Agropyron cristatum subsp. incanum. The relationships between morphological variations observed among the taxa and ploidy levels were also discussed. | ||
| کلیدواژهها | ||
| Flow cytometry؛ ploidy determination؛ nuclear DNA content؛ agropyron cristatum | ||
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