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Measuring of free endotoxin in alum-precipitated vaccine of haemorrhagic septicaemia by limulus amebocyte lysate test | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 13، دوره 8، شماره 1 - شماره پیاپی 18، 2007، صفحه 83-85 اصل مقاله (20.76 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2007.2713 | ||
| نویسندگان | ||
| A. R. Jabbari* ؛ G. R. Moazeni Jula | ||
| Department of Aerobic Veterinary Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Karadj, Iran | ||
| چکیده | ||
| Haemorrhagic septicaemia (HS) vaccine which is prepared in Razi Institute is used in endemic areas of Iran. Aluminum-hydroxide gel was used as adjuvant for preparing this vaccine. Post-vaccinal shock reactions were the main complaint after use of this vaccine. In a previous study, we could improve the vaccine by alum-precipitation Pasteurella multocida cells and removing the liquid phase. In this study, the amount of free endotoxin in aluminum-hydroxide and alum-HS vaccines was determined. It was found that endotoxin level was considerably decreased from 0.22 EU/ml to 0.03 EU/ml after alum-precipitation. | ||
| کلیدواژهها | ||
| Haemorrhagic septicaemia vaccine؛ Endotoxin؛ Shock reaction؛ LAL test | ||
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