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Pathogenicity and immunogenicity of native and mutant strains of Pasteurella multocida, the causative agents of haemorrhagic septicaemia | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 6، دوره 8، شماره 1 - شماره پیاپی 18، 2007، صفحه 40-44 اصل مقاله (133.69 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2007.2706 | ||
| نویسندگان | ||
| M. Tabatabaei* 1؛ G. R. Moazzeni Jula2؛ A. R. Jabbari2؛ M. Esmailzadeh3 | ||
| 1Department of Microbiology, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran | ||
| 2Department of Aerobic Vaccine Production, Razi Vaccine and Serum Research Institute, Karadj, Iran | ||
| 3Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karadj, Iran | ||
| چکیده | ||
| Haemorrhagic septicaemia (HS) is a fatal systemic disease of cattle and buffaloes. Some control is achieved with administration of alum-precipitated or oil-adjuvanted killed whole-cell vaccines injected subcutaneously. These vaccines, however, provide only short-term immunity and for effective use, they should be administered annually. We constructed an aroA attenuated derivative of a Pasteurella multocida serotype B:2 strain by allelic exchange of the native aroA sequence with aroA sequences disrupted with a kanamycin resistance cassette. This strain was confirmed to be aroA mutant by PCR. The aroA derivative was highly attenuated for virulence in a mouse model of HS and rabbits. Mouse and rabbit challenge experiments showed that i.p. or i.m. vaccination of an aroA strain completely protected mice or rabbits against challenge with a high dose (>1000 LD50) of the parent strain. | ||
| کلیدواژهها | ||
| Pasteurella multocida؛ Live vaccine؛ aroA mutant؛ Haemorrhagic septicaemia | ||
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