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Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 4، دوره 7، شماره 1 - شماره پیاپی 14، 2006، صفحه 17-21 اصل مقاله (38.85 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2006.2675 | ||
| نویسندگان | ||
| M. R. Sadeghi1؛ S. A. Ghorashi* 1؛ R. Kargar Moakhar2؛ D. Morshedi1؛ R. Salehi Tabar1؛ S. S. Ghaemmaghami3 | ||
| 1National Institute for Genetic Engineering and Biotechnology, Tehran, Iran | ||
| 2Razi Vaccine and Serum Research Institute, Karadj, Iran | ||
| 3Veterinary Medicine Research Center, Agriculture Organization, Markazi Province, Arak, Iran | ||
| چکیده | ||
| Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocytic infiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) to optimize a rapid and reliable assay for detection of MDV genome. Detection of serotype 1 of MDV (MDV-1) was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 and herpesvirus of turkeys (HVT) was also conducted using specific primers from the glycoprotein A (gA) gene and a 388 bp DNA fragment was amplified from HVT genome. The specificity of the test was confirmed by sequencing of PCR products. Results indicate that MDV-1 can be diagnosed in clinical samples and inoculated cell cultures which is used for virus isolation. In addition, differentiation between MDV-1 and HVT viruses was confirmed based on the size of PCR products. The test proved to be rapid and reliable and can be performed as a robust diagnostic test in veterinary diagnostic laboratories. | ||
| کلیدواژهها | ||
| Marek’s disease virus؛ PCR؛ Detection | ||
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