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Sadeghi, M. R., Ghorashi, S. A., Kargar Moakhar, R., Morshedi, D., Salehi Tabar, R., Ghaemmaghami, S. S.. (1384). Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT. , 7(1), 17-21. doi: 10.22099/ijvr.2006.2675
M. R. Sadeghi; S. A. Ghorashi; R. Kargar Moakhar; D. Morshedi; R. Salehi Tabar; S. S. Ghaemmaghami. "Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT". , 7, 1, 1384, 17-21. doi: 10.22099/ijvr.2006.2675
Sadeghi, M. R., Ghorashi, S. A., Kargar Moakhar, R., Morshedi, D., Salehi Tabar, R., Ghaemmaghami, S. S.. (1384). 'Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT', , 7(1), pp. 17-21. doi: 10.22099/ijvr.2006.2675
Sadeghi, M. R., Ghorashi, S. A., Kargar Moakhar, R., Morshedi, D., Salehi Tabar, R., Ghaemmaghami, S. S.. Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT. , 1384; 7(1): 17-21. doi: 10.22099/ijvr.2006.2675

Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT

Iranian Journal of Veterinary Research
مقاله 4، دوره 7، شماره 1 - شماره پیاپی 14، 2006، صفحه 17-21    اصل مقاله (38.85 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2006.2675
نویسندگان
M. R. Sadeghi1؛ S. A. Ghorashi* 1؛ R. Kargar Moakhar2؛ D. Morshedi1؛ R. Salehi Tabar1؛ S. S. Ghaemmaghami3
1National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
2Razi Vaccine and Serum Research Institute, Karadj, Iran
3Veterinary Medicine Research Center, Agriculture Organization, Markazi Province, Arak, Iran
چکیده
Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocytic
infiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) to
optimize a rapid and reliable assay for detection of MDV genome. Detection of serotype 1 of MDV (MDV-1)
was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 and
herpesvirus of turkeys (HVT) was also conducted using specific primers from the glycoprotein A (gA) gene
and a 388 bp DNA fragment was amplified from HVT genome. The specificity of the test was confirmed by
sequencing of PCR products. Results indicate that MDV-1 can be diagnosed in clinical samples and inoculated cell cultures which is used for virus isolation. In addition, differentiation between MDV-1 and HVT viruses was confirmed based on the size of PCR products. The test proved to be rapid and reliable and can be performed as a robust diagnostic test in veterinary diagnostic laboratories.
کلیدواژه‌ها
Marek’s disease virus؛ PCR؛ Detection
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