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Hashemzadeh Farhang, H., Nabavi, L., Seyfiabad Shapouri, M. R., Rahbari, S., Azizi, F.. (1385). Development of an ELISA technique for the detection of Babesia ovis and serological survey of the parasite in Khouzestan province, southern Iran. , 7(2), 53-58. doi: 10.22099/ijvr.2006.2663
H. Hashemzadeh Farhang; L. Nabavi; M. R. Seyfiabad Shapouri; S. Rahbari; F. Azizi. "Development of an ELISA technique for the detection of Babesia ovis and serological survey of the parasite in Khouzestan province, southern Iran". , 7, 2, 1385, 53-58. doi: 10.22099/ijvr.2006.2663
Hashemzadeh Farhang, H., Nabavi, L., Seyfiabad Shapouri, M. R., Rahbari, S., Azizi, F.. (1385). 'Development of an ELISA technique for the detection of Babesia ovis and serological survey of the parasite in Khouzestan province, southern Iran', , 7(2), pp. 53-58. doi: 10.22099/ijvr.2006.2663
Hashemzadeh Farhang, H., Nabavi, L., Seyfiabad Shapouri, M. R., Rahbari, S., Azizi, F.. Development of an ELISA technique for the detection of Babesia ovis and serological survey of the parasite in Khouzestan province, southern Iran. , 1385; 7(2): 53-58. doi: 10.22099/ijvr.2006.2663

Development of an ELISA technique for the detection of Babesia ovis and serological survey of the parasite in Khouzestan province, southern Iran

Iranian Journal of Veterinary Research
مقاله 7، دوره 7، شماره 2 - شماره پیاپی 15، 2006، صفحه 53-58    اصل مقاله (48.42 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2006.2663
نویسندگان
H. Hashemzadeh Farhang1؛ L. Nabavi* 2؛ M. R. Seyfiabad Shapouri3؛ S. Rahbari4؛ F. Azizi5
1Department of Parasitology, Faculty of Veterinary Medicine, Islamic Azad University of Tabriz, Tabriz, Iran
2Department of Parasitology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3Department of Microbiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
5Department of Mathematic and Statistics, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده
To develop an ELISA technique for the detection of antibodies against Babesia ovis, the infected
erythrocytes were lysed and the supernatant soluble antigen, after sonication and ultracentrifugation of the
lysate was used as antigen. Optimal dilution of the antigen was determined by checkerboard titrations, using
positive and negative control sera. A correlation of 85% was observed between the results of the developed
ELISA and IFA techniques. To study the seroprevalence of Babesia ovis in Khouzestan province, south of
Iran, a total of 1000 sheep sera were collected from different areas of the province and tested against Babesia ovis using the ELISA technique developed. The results showed an average seroprevalence of 47.5% in the province. Our results indicated a significant increase of the seroprevalence by advancement of age of the animals. There was no significant difference between the seroprevalence of female and male sheep.
کلیدواژه‌ها
Babesia ovis؛ ELISA؛ Seroprevalence
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