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Polymerase chain reaction typing of Pasteurella multocida capsules isolated in Iran | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 9، دوره 7، شماره 3 - شماره پیاپی 16، 2006، صفحه 50-55 اصل مقاله (42.46 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2006.2649 | ||
| نویسندگان | ||
| A. R. Jabbari* 1؛ M. Esmaelzadeh2؛ Gh. R. Moazeni Jula3 | ||
| 1Department of Veterinary Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karadj, Iran | ||
| 2Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karadj, Iran | ||
| 31Department of Veterinary Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karadj, Iran | ||
| چکیده | ||
| Capsules from a range of pathogenic bacteria are the key determinants of virulency. The capsule has been implicated in virulence of Pasteurella multocida. In this study a type-specific polymerase chain reaction (PCR) assay was used for capsular typing of 39 avian P. multocida isolates from Iran. The PCR amplified a fragment of 1044 bp from all of tested isolates. It was found that all avian P. multocida isolates belonged to capsular type A. The sequence alignment of the fragment showed a high similarity (>96%) with the published sequences of P. multocida hya gene in the Gene Bank. It was recognised that P. multocida capsular group A is the dominant cause of fowl cholera in Iran. | ||
| کلیدواژهها | ||
| P. multocida؛ Capsule؛ PCR؛ Typing | ||
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