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Molecular phylogeny of Myxobolus orissae (Myxosporea: Myxobolidae) infecting the gill lamellae of mrigal carp Cirrhinus mrigala (Actinopterygii: Cyprinidae) | ||
| Molecular Biology Research Communications | ||
| مقاله 2، دوره 4، شماره 1، خرداد 2015، صفحه 15-24 اصل مقاله (989.67 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2015.2547 | ||
| نویسندگان | ||
| Thangapalam Abraham* 1؛ Sayani Banerjee2؛ Avijit Patra3؛ Agniswar Sarkar3؛ Harresh Adikesavalu3؛ Gadadhar Dash3 | ||
| 1Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India | ||
| 2Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata | ||
| 3Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, India | ||
| چکیده | ||
| Myxosporeans are best known for the diseases they cause in commercially important fish species. Identification of myxosporeans at the species-level is mainly based on conventional methods. The 18S rRNA gene sequence of morphologically identified Myxobolus orissae infecting the gill lamellae of mrigal carp Cirrhinus mrigala was characterized in the present study. The plasmodia of M. orissae were small, elongated and white to pale in colour. Phylogenetically, the 18S rDNA nucleotide sequence of M. orissae was clustered with other gill-infecting Myxobolus spp. of cyprinids. The species closely related to M. orissae was M. koi (FJ841887) infecting the gill lamellae of Cyprinus carpio with 96% similarity. The carp fin-infecting Thelohanellus caudatus (KC865607) from India exhibited only 78% DNA sequence similarity with M. orissae. Low level of M. orissae infection on gill caused thickening of epithelial cells surrounding the plasmodium. Under stressful conditions, it is likely that such infection can easily spread in confined fish and may cause serious disease outbreaks and economical losses. | ||
| کلیدواژهها | ||
| Cirrhinus mrigal؛ Myxobolus orissae؛ Molecular characterization؛ Phylogenetic relationship | ||
| مراجع | ||
|
1. Lom J, Dyková I. Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol 2006;53:1-36.
2. Lom J, Dykova I. Myxosporea (Phylum Myxozoa). In: Woo PTK (ed) Fish Diseases and Disorders, Vol. 1, Protozoan and Metazoan Infections. CAB International, Wallingford 1995; PP 97-148.
3. Kent ML, Andree KB, Bartholomew JL, El-Matbouli M, Desser SS, Devlin RH, Feist SW, Hedrick RP, Hoffmann RW, Khattra J, Hallett SL, Lester RJG, Longshaw M, Palenzeula O, Siddall ME, Xiao C. Recent advances in our knowledge of the Myxozoa. J Eukary Microbiol 2001;48:395-413.
4. Kaur H, Singh R. A synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Bivalvulida) parasitizing Indian fishes and a revised dichotomous key to myxosporean genera. Syst Parasitol 2012;81:17-37.
5. Yokoyama H, Grabner D, Shirakashi S. Transmission Biology of the Myxozoa. In: Health and Environment in Aquaculture. Carvalho ED, David GS, Silva RJ (eds), InTech, Croatia 2012; PP 1-42. ISBN: 978-953-51-0497-1, Available from http://www.intechopen.com/books/health-and-environment-in-aquaculture/ transmission-biology-of-the-myxozoa (Accessed on 30 May, 2013).
6. Smothers JF, Van Dohlen CD, Smith Jr LH, Spall RD. Molecular evidence that the myxozoan protists are metazoans. Science 1994;265:1719-1721.
7. Eszterbauer E. Genetic relationship among gill-infecting Myxobolus species (Myxosporea) of cyprinids: molecular evidence of importance of tissue-specificity. Dis Aquat Org 2004;58:35-40.
8. Fiala I. The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 2006;36:1521-1534.
9. Molnár K, Marton S, Székely C, Eszterbauer E. Differentiation of Myxobolus spp. (Myxozoa: Myxobolidae) infecting roach (Rutilus rutilus) in Hungary. Parasitol Res 2010;107:1137-1150.
10. Cech G, Molnár K, Székely C. Molecular genetic studies on morphologically indistinguishable Myxobolus spp. infecting cyprinid fishes, with the description of three new species, M. alvarezae sp. nov., M. sitjae sp. nov. and M. eirasianus sp. nov. Acta Parasitol 2012;57:354-366.
11. Xi BW, Xie J, Zhou QL, Pan LK, Ge XP. Mass mortality of pond-reared Carassius gibelio caused by Myxobolus ampullicapsulatus in China. Dis Aquat Org 2011;93: 257-260.
12. Mondal A, Banerjee S, Patra A, Adikesavalu H, Ramudu KR, Dash G, Joardar SN, Abraham TJ. Molecular and morphometric characterization of Thelohanellus caudatus (Myxosporea: Myxobolidae) infecting the caudal fin of Labeo rohita (Hamilton). Protistol 2014;8:41-52.
13. Rajesh SC, Banerjee S, Patra A, Dash G, Abraham TJ. Molecular characterization of Myxobolus cuttacki (Myxozoa, Myxosporea, Bivalvulida) infecting gill lamellae of minor carp Labeo bata (Ham.). Mol Biol Res Commun 2014;3:1-9.
14. Lom J, Arthur JR. A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 1989;12:151-156.
15. Lom J, Vavrá J. Mucous envelopes of spores of the subphylum Cnidospora (Deflein, 1901). Vestn Cesk Spol Zool 1963;27:4-6.
16. Barta JR, Martin DS, Liberator PA, Dashkevicz M, Anderson JW, Feighner SD, Elbrecht A, Perkins-Barrow A, Jenkins MC, Danforth HD, Ruff MD, Profous-Juchelka H. Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. J Parasitol 1997;83:262-271.
17. Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111-120.
18. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-2739.
19. Ronquist F, Huelsenbeck JP. Bayesian phylogenetic inference under mixed models. Biogeosciences 2003;19:1572-1574
20. Singh R, Kaur H. Thelohanellus (Myxozoa: Myxosporea: Bivalvulida) infections in major carp fish from Punjab wetlands (India). Protistol 2012;7:178-188.
21. Singh R, Kaur H. Biodiversity of myxozoan parasites infecting freshwater fishes of three main wetlands of Punjab, India. Protistol 2012;7:79-89.
22. Haldar DP, Samal KK, Mukhopadhyaya D. Studies on protozoan parasites of fishes in Orissa: Eight species of Myxobolus Bütschli (Myxozoa: Bivalvulida). J Bengal Nat History Soc 1996;16:3-24.
23. Molnár K. Comments on the host, organ and tissue specificity of fish myxosporeans and the types of their intrapiscine development. Parasitol Hung 1994;27:5-20. | ||
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