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Molecular phylogeny of Myxobolus orissae (Myxosporea: Myxobolidae) infecting the gill lamellae of mrigal carp Cirrhinus mrigala (Actinopterygii: Cyprinidae) | ||
| Molecular Biology Research Communications | ||
| مقاله 2، دوره 4، شماره 1، خرداد 2015، صفحه 15-24 اصل مقاله (989.67 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2015.2547 | ||
| نویسندگان | ||
| Thangapalam Abraham* 1؛ Sayani Banerjee2؛ Avijit Patra3؛ Agniswar Sarkar3؛ Harresh Adikesavalu3؛ Gadadhar Dash3 | ||
| 1Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India | ||
| 2Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata | ||
| 3Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, India | ||
| چکیده | ||
| Myxosporeans are best known for the diseases they cause in commercially important fish species. Identification of myxosporeans at the species-level is mainly based on conventional methods. The 18S rRNA gene sequence of morphologically identified Myxobolus orissae infecting the gill lamellae of mrigal carp Cirrhinus mrigala was characterized in the present study. The plasmodia of M. orissae were small, elongated and white to pale in colour. Phylogenetically, the 18S rDNA nucleotide sequence of M. orissae was clustered with other gill-infecting Myxobolus spp. of cyprinids. The species closely related to M. orissae was M. koi (FJ841887) infecting the gill lamellae of Cyprinus carpio with 96% similarity. The carp fin-infecting Thelohanellus caudatus (KC865607) from India exhibited only 78% DNA sequence similarity with M. orissae. Low level of M. orissae infection on gill caused thickening of epithelial cells surrounding the plasmodium. Under stressful conditions, it is likely that such infection can easily spread in confined fish and may cause serious disease outbreaks and economical losses. | ||
| کلیدواژهها | ||
| Cirrhinus mrigal؛ Myxobolus orissae؛ Molecular characterization؛ Phylogenetic relationship | ||
| مراجع | ||
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