| تعداد نشریات | 24 |
| تعداد شمارهها | 859 |
| تعداد مقالات | 7,646 |
| تعداد مشاهده مقاله | 13,578,496 |
| تعداد دریافت فایل اصل مقاله | 11,684,914 |
Differential expression of BnSRK2D gene in two Brassica napus cultivars under water deficit stress | ||
| Molecular Biology Research Communications | ||
| مقاله 4، دوره 3، شماره 4، اسفند 2014، صفحه 241-251 اصل مقاله (701.95 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2014.2494 | ||
| نویسندگان | ||
| Bahlanes Bakhtari1؛ Hooman Razi* 2 | ||
| 1Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Iran | ||
| 2Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University | ||
| چکیده | ||
| The sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family members are plant unique serine/threonine kinases which play a key role in cellular signaling in response to abiotic stresses. The three SnRK2 members including SRK2D, SRK2I and SRK2E are known to phosphorylate major abscisic acid (ABA) responsive transcription factors, ABF2 and ABF4, involved in an ABA-dependent stress signaling pathway in Arabidopsis. This study aimed to clone and sequence an ortholog of the Arabidopsis SRK2D gene from Brassica napus, designated as BnSRK2D. An 833bp cDNA fragment of BnSRK2D, which shared high amino acid sequence identity with its Arabidopsis counterpart, was obtained suggesting a possible conserved function for these genes. The expression pattern of BnSRK2D and its potential target gene B. napus ABF2 (BnABF2) were then analyzed in the two cultivars with contrasting reaction to water deficit stress. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) showed that BnSRK2D and BnABF2 were water-deficit stress responsive genes with similar expression profiles. The accumulation of the BnSRK2D and BnABF2 transcripts in the two cultivars was linked with their level of drought tolerance, as the drought tolerant cultivar had significantly higher expression levels of both genes under normal and water deficit stress conditions. These findings suggest that BnSRK2D and BnABF2 genes may be involved in conferring drought tolerance in B. napus. | ||
| کلیدواژهها | ||
| Rapeseed؛ SnRK2؛ BnABF2؛ Drought tolerance؛ Transcript accumulation | ||
| مراجع | ||
|
1.Yang Y, Shah J, Klessig DF. Signal perception and transduction in plant defense responses. Genes Dev 1997;11:1621-1639.
2.Kulik A, Wawer I, Krzywińska E, Bucholc M, Dobrowolska G. SnRK2 protein kinases- key regulators of plant response to abiotic stresses. OMICS 2011;15:859-972.
3.Wrzaczek M, Hirt H. Plant MAP kinase pathways: how many and what for? Biol Cell 2001;93:81-87.
4.Zhang T, Liu Y, Yang T, Zhang L, Xu L, An L. Diverse signals converge at MAPK cascades in plants. Plant Physiol Biochem 2006;44: 274-283.
5.Rodriguez MC, Petersen M, Mundy J. Mitogen-activated protein kinase signaling in plants. Annu Rev Plant Biol 2010; 61:621–649.
6.Ludwig A, Romeis T, Jones JD. CDPK mediated signaling pathways: specificity and cross-talk. J Exp Bot 2004;55:181-8.
7.Das R, Pandey GK. Expressional analysis and role of calcium sensing kinases in abiotic stress signaling. Curr Genomics 2010;11:2-13.
8.Hrabak EM, Chan CW, Gribskov M, Harper JF, Choi JH, Halford N, Kudla J, Luan S, Nimmo HG, Sussman MR, Thomas M, Walker-Simmons K, Zhu JK, Harmon AC. The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol 2003;132:666-680.
9.Halford NG, Hey SJ. SNF1-related protein kinases (SnRKs) act within an intricate network that links metabolic and stress signaling in plants. Biochem J 2009;419: 247-259.
10.Hey SJ, Byrne E, Halford NG. The interface between metabolic and stress signaling. Ann Bot 2010;105:197-203.
11.Hardie DG, Carling D, Carlson M. The AMP activated/SNF1 protein kinase subfamily: metabolic sensors of the eukaryotic cell? Annu Rev Biochem 1998;67: 821-855.
12.Halford NG, Hey S, Jhurreea D, Laurie S, Mckibbin RS, Paul M and Zhang Y. Metabolic signaling and carbon partitioning: Role of Snf1-related (SnRK1) protein kinase. J Exp Bot 2003;54:467-475.
13.Fujii H,VersluesPE, Zhu JK. Arabidopsis decuple mutant reveals the importance of SnRK2 kinases in osmotic stress responses in vivo. Proc Natl Acad Sci USA 2011; 108:1717-1722.
14.Anderberg RJ, Walker-Simmons MK. Isolation of a wheat cDNA clone for an abscisic acid-inducible transcript with homology to protein kinases. Proc Natl Acad Sci USA 1992;89:10183-10187.
15.Li J, Assmann SM. An abscisic acid-activated and calcium-independent protein kinase from guard cells of fava bean. Plant Cell 1996;8:2359-2368.
16.Li J, Wang XQ,WatsonMB, Assmann SM. Regulation of abscisic acid-induced stomatal closure and anion channels by guard cell AAPK kinase. Science 2000;287: 300-303.
17.Boudsocq M, Barbier-Brygoo H, Laurière C. Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana. J Biol Chem 2004;279:41758-41766.
18.Boudsocq M, Droillard M, Barbier-Brygoo H, Laurière C. Different phosphorylati-on mechanisms are involved in the activation of sucrose non-fermenting 1 related protein kinases 2 by osmotic stresses and abscisic acid. Plant Mol Biol 2007;63:491-503.
19.Mustilli AC, Merlot S, Vavasseur A, Fenzi F, Giraudat J. Arabidopsis OST1 protein kinase mediates the regulation of stomatal aperture by abscisic acid and acts upstream of reactive oxygen species production. Plant Cell 2002;14:3089-3099.
20.Umezawa T, Yoshida R, Maruyama K, Yamaguchi-Shinozaki K, Shinozaki K. SRK2C, a SNF1-related protein kinase 2, improves drought tolerance by controlling stress-responsive gene expression in Arabidopsis thaliana. Proc Natl Acad Sci USA 2004;101:17306-17311.
21.Mcloughlin F, Galvan-Ampudia CS, Julkowska MM, Caarls L, Van Der Does D, Lauriere C, Munnik T, Haring MA, Testerink C. The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress. Plant J 2012;72:436-449.
22.Fujita Y, Nakashima K, Yoshida T, Katagiri T, Kidokoro S, Kanamori N, Umezawa T, Fujita M, Maruyama K, Ishiyama K, Kobayashi M, Nakasone S, Yamada K, Ito T, Shinozaki K, Yamaguchi-Shinozaki K. Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis. Plant Cell Physiol 2009;50:2123-2132.
23.Fujii H, Zhu J. Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stress. Proc Natl Acad Sci USA 2009;106:8380-8385.
24.Nakashima K, Fujita Y, Kanamori N, Katagiri T, Umezawa T, Kidokoro S, Maruyama K, Yoshida T, Ishiyama K, Kobayashi M, Shinozaki K, Yamaguchi-Shinozaki K. Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved inABAsignaling are essential for the control of seed development and dormancy. Plant Cell Physiol 2009;50:1345-1363.
25.Furihata T, Maruyama K, Fujita Y, Umezawa T, Yoshida R, Shinozaki K. Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1. Proc Natl Acad Sci USA 2006;103:1988-1993.
26.Choi H, Hong J, Ha J, Kang J, Kim SY. ABFs, a family of ABA-responsive element binding factors. J Biol Chem 2000;275:1723-1730.
27.Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc Natl Acad Sci USA 2000;97:11632-11637.
28.Kobayashi Y, Yamamoto S, Minami H, Kagaya Y, Hattori T. Differential activation of the rice sucrose non fermenting 1 related protein kinase 2 family by hyperosmotic stress and abscisic acid. Plant Cell 2004;16:1163-1177.
29.Huai J. Wang M. He J. Zheng J. Dong Z. Lv H, Zhao J, Wang G. Cloning and characterization of the SnRK2 gene family from Zea mays. Plant Cell Rep 2008;12: 1861-1868.
30.Zhang H, Mao X, Jing R, Chang X, Xie H. Characterization of a common wheat (Triticum aestivum L.) TaSnRK2.7 gene involvement in abiotic stress responses. J Exp Bot 2011;62:975-988.
31.Tian DQ, Pan XY, Yu YM, Wang WY, Zhang F, Ge YY, Shen XL, Shen FQ, Liu XJ. De novo characterization of the Anthurium transcriptome and analysis of its digital gene expression under cold stress. BMC Genomics 2013;14:827.
32.Monks DE, Aghoram K, Courtney PD, DeWald DB, Dewey RE. Hyperosmotic stress induces the rapid phosphorylation of a soybean phosphatidylinositol transfer protein homolog through activation of the protein kinases SPK1 and SPK2. Plant Cell 2001;13:12005-12019.
33.Yoon HW, Kim MC, Shin PG, Kim JS, Kim CY, Lee SY, Hwang I, Bahk JD, Hong JC, Han C. Differential expression of two functional serine/threonine protein kinases from soyabean that have an unusual acidic domain at the carboxy terminus. Mol Gen Genet 1997;255:359-371.
34.Kelner A, Pękala I, Kaczanowski S, Muszyńska G, Hardie D.G, Dobrowolska G, Biochemical characterization of the Nicotiana tabacum 42-kD protein kinase activated by osmotic stress. Plant Physiol 2004;136:3255-3264.
35.Mao X, Zhang H, Tian S, Chang X, Jing R. TaSnRK2.4, an SNF1-type serine/ threonine protein kinase of wheat (Tricitum aestivum L.), confers enhanced multistress tolerance in Arabidopsis. J Exp Bot 2010;61:683-696.
36.Zhang H, Mao X, Wang C, Jing R. Overexpression of a common wheat gene TaSnRK2.8 enhances tolerance to drought, salt and low temperature in Arabidopsis. PLoS One 2010;5:e16041.
37.Diédhiou CJ, Popova OV, Dietz KJ, Golldack D. The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice. BMC Plant Biol 2008;8:49.
38.Zou H, Li C, Liu H, Zhao M, Tian X, Ma G, Li Z. ZmSPK1, a member of plant SnRK2 subfamily in maize enhances tolerance to salt in transgenic Arabidopsis. Aust J Crop Sci 2011;5:1179-1184.
39.Bakhtari B. Isolation of a member of SnRK2 gene family encoding a protein kinase putatively involved in drought tolerance in Brassica napus. M.Sc. Thesis 2012;ShirazUniversity.
40.Rana D, Boogaart TVD, O' Neill CM, Hynes L, Bent E, Macpherson L, Par JY, Lim YP, Bancroft L. Conservation of the microstructure of genome segments in Brassica napus and its diploid relatives. Plant J 2004;40:725-733.
41.Mun JH, Kwon SJ, Seol YJ, Kim JA, Jin M, Kim JS, Lim MH, Lee SI, Hong JK, Park TH, Lee SC, Kim BJ, Seo MS, Baek S, Lee MJ, Shin JY, Hahn JH, Hwang YJ, Lim KB, Park JY, Lee J, Yang TJ, Yu HJ, Choi IY, Choi BS, Choi SR, Ramchiary N, Lim YP, Fraser F, Drou N, Soumpourou E, Trick M, Bancroft I, Sharpe AG, Parkin IAP, Batley J, Edwards D, Park BS. Sequence and structure of Brassica rapa chromosome A3. Genome Biol 2010;11:R94. | ||
|
آمار تعداد مشاهده مقاله: 4,807 تعداد دریافت فایل اصل مقاله: 4,599 |
||