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Molecular characterization of Myxobolus cuttacki (Myxozoa, Myxosporea, Bivalvulida) infecting gill lamellae of minor carp Labeo bata (Ham.) | ||
| Molecular Biology Research Communications | ||
| مقاله 3، دوره 3، شماره 4، اسفند 2014، صفحه 231-239 اصل مقاله (740.73 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2014.2493 | ||
| نویسندگان | ||
| Sandya Chinna Rajesh1؛ Sayani Banerjee2؛ Avijit Patra2؛ Gadadhar Dash2؛ Thangapalam Abraham* 3 | ||
| 1Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata | ||
| 2Department of Aquatic Animal Health, Faculty of Fishery Sciences, WBUAFS, Kolkata | ||
| 3Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata - 700 094, West Bengal, India | ||
| چکیده | ||
| As new pathogenic strains are emerging and threatening aquaculture development, myxosporeans (Myxozoa) are receiving much attention in recent years. Myxosporean taxonomy is traditionally based on morphology of the myxospore stage. Molecular data on Indian myxosporeans are rare. In this report, the 18S rRNA gene sequence of Myxobolus cuttacki infecting gill lamellae of minor carp Labeo bata (Ham.) and its phylogenetic relationship with other myxobolids are described for the first time. The plasmodia of M. cuttacki were 0.5-0.9 mm in size and whitish with a round to oval shape. The mean mature spore size was 16.10×7.05 μm. The 18S rRNA nucleotide sequence with 1703 bp of M. cuttacki (accession number KF465682) clustered phylogenetically with other Myxobolus spp. infecting cyprinid gills with 78-90% homogeneity. The gill lamellae infecting M. catmrigalae (KC933944) and M. orissae (KF448527) of Indian major carp Cirrhinus mrigala from India, exhibited 86% and 81% homogeneity with M. cuttacki, respectively. The infection rate was low to moderate on the gills which can have a negative impact on respiratory and physiological functions and subsequently on fish production. | ||
| کلیدواژهها | ||
| Labeo bata؛ Myxobolus cuttacki؛ 18S rRNA؛ Phylogenetic relationship | ||
| مراجع | ||
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1.Molnár K. Comments on the host, organ and tissue specificity of fish myxosporeans and the types of their intrapiscine development. Parasitol Hung 1994;27:5-20.
2.Molnár K. Differences between the European carp (Cyprinus carpio carpio) and the coloured carp (Cyprinus carpio haematopterus) in susceptibility to Thelohanellus nikolskii (Myxosporea) infection. Acta Vet Hung 2002;50:51-57.
3.Kent ML, Andree KB, Bartholomew JL, El-Matbouli M, Desser SS, Devlin RH, Feist SW, Hedrick RP, Hoffmann RW, Khattra J, Hallett SL, Lester RJG, Longshaw M, Palenzeula O, Siddall ME, Xiao C. Recent advances in our knowledge of the Myxozoa. J Eukary Microbiol 2001;48:395-413.
4.Eszterbauer E. Genetic relationship among gill-infecting Myxobolus species (Myxosporea) of cyprinids: molecular evidence of importance of tissue-specificity. Dis Aquat Org 2004;58:35-40.
5.Fiala I. The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 2006;36:1521-1534.
6.Molnár K, Marton S, Székely C, Eszterbauer E. Differentiation of Myxobolus spp. (Myxozoa: Myxobolidae) infecting roach (Rutilus rutilus) in Hungary. Parasitol Res 2010;107:1137-1150.
7.Yokoyama H, Grabner D, Shirakashi S. Transmission biology of the Myxozoa. In: Carvalho ED, David GS, Silva RJ (eds) Health and environment in aquaculture, China: InTech, Crotia 2012; PP 1-42. Available from: http://www.intechopen. com/ books/health-and-environment-in-aquaculture/transmission-biology-of-the myxozoa. (Accessed on 30 May, 2013)
8.Basu S, Haldar DP. Description of three new myxosporean species (Myxozoa: Myxosporea: Bivalvulida) of the genera Myxobilatus Davis, 1944 and Myxobolus Bütschli, 1882. Acta Protozool 2004;43:337-343.
9.Kaur H, Singh R. A synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Bivalvulida) parasitizing Indian fishes and a revised dichotomous key to myxosporean genera. Syst Parasitol 2012;81:17-37.
10.Singh R, Kaur H. Thelohanellus (Myxozoa: Myxosporea: Bivalvulida) infections in major carp fish from Punjab wetlands (India). Protistol 2012;7:178-188.
11.Singh R, Kaur H. Biodiversity of myxozoan parasites infecting freshwater fishes of three main wetlands of Punjab, India. Protistol 2012;7:79-89.
12.Mondal A, Banerjee S, Patra A, Adikesavalu H, Ramudu KR, Dash G, Joardar SN, Abraham TJ. Molecular and morphometric characterization of Thelohanellus caudatus (Myxosporea: Myxobolidae) infecting the caudal fin of Labeo rohita (Hamilton). Protistol 2014;8:41-52.
13.Lom J, Arthur JR. 1989. A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 1989;12:151-156.
14.Barta JR, Martin DS, Liberator PA, Dashkevicz M, Anderson JW, Feighner SD, Elbrecht A, Perkins-Barrow A, Jenkins MC, Danforth HD, Ruff MD, Profous-Juchelka H. Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. J Parasitol 1997;83: 262-271.
15.Thompson JD, Gibson JJ, Plewniak F, Jeanmougin F, Higgins DG. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;24:4876-4882.
16.Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-2739.
17.Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111-120.
18.Felsenstein J. Phylogenies and the method. Am Nat 1985;125:1-15.
19.Haldar DP, Samal KK, Mukhopadhyaya D. Studies on protozoan parasites of fishes in Orissa: Eight species of Myxobolus Bütschli (Myxozoa: Bivalvulida). J Bengal Nat His Soc 1996;16:3-24.
20.Moshu A, Molnar K. Thelohanellus (Myxozoa: Myxosporea) infection of the scales in the European wild carp Cyprinus carpio carpio. Dis Aquat Org 1997;28:115-123. | ||
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