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Isolation and characterization of Phi class glutathione transferase partial gene from Iranian barley | ||
| Molecular Biology Research Communications | ||
| مقاله 4، دوره 1، شماره 1، آذر 2012، صفحه 21-26 اصل مقاله (354.66 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2012.237 | ||
| نویسندگان | ||
| Sasan Mohsenzadeh* 1؛ Maryam Esmaeili1؛ Hassan Mohabatkar2 | ||
| 1Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran | ||
| 2Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran | ||
| چکیده | ||
| Glutathione transferases are multifunctional proteins involved in several diverse intracellular events such as primary and secondary metabolisms, signaling and stress metabolism. These enzymes have been subdivided into eight classes in plants. The Phi class, being plant specific, is the most represented. In the present study, based on the sequences available at GenBank, different primers were designed for amplifying the Phi class of glutathione transferase gene in the genome and transcriptome of Iranian barley, Karoun cultivar. After extraction of DNA and total RNA, Phi class was amplified and sequenced. Bioinformatics analysis predicted that the deduced protein sequence has two ß-sheets, eight α-helices and some intermediate loops in its secondary structure. Consequently, the sequences were submitted to NCBI GenBank with GS262333 and GW342614 accession numbers. Phylogenic relationships of the sequences were compared with existing sequences in GenBank. | ||
| کلیدواژهها | ||
| Hordeum vulgare؛ Glutathione S-transferase؛ stress؛ Xenobiotics | ||
| مراجع | ||
|
1. Edwards R, Dixon DP, Walbot V. Plant glutathione S-transferase: enzymes with multiple functions in sickness and in health. Trends Plant Sci 2000;5:193-198.
2. Dixon D, Lapthorn A, Edwards R. Plant glutathione transferases. Genome Biol 2002; 3:3004.
3. Wilce MCJ, Parker MW. Structure and function of glutathione S-transferases. Biochim Biophys Acta 1994;1205:1-18.
4. Wongsantichon J, Ketterman A. Alternative splicing of glutathione transferases. Method Enzymol 2005;401:100-116.
5. Hayes JD, Pulford DJ. The glutathione S-transferase super gene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol 1995;30:445-600.
6. Oztetik E (2008) A tale of plant glutathione Stransferases: since 1970. Bot Rev 74: 419-437.
7. Mohsenzadeh S, Saffari B, Mohabatkar H. A new member of Tau-class glutathione S-transferase from barley leaves. EXCLI Journal 2009;8:190-194.
8. McGonigle B, Keeler SJ, Lau SMC, Koppe MK, Okeefe DP. A genomics approach to the comprehensive analysis of the glutathione S-transferase gene family in soybean and maize. Plant Physiol 2000;124:1105-1120.
9. Basantani M, Srivastava A. Plant glutathione transferases - a decade falls short. Can J Bot 2007;85:443-456.
10. Noctor G, Arisi A, Jouanin L, Kunert K, Rennenberg H, Foyer C. Glutathione: biosynthesis metabolism and relationship to stress tolerance explored in transformed plants. J Exp Bot 1998;49:623-647.
11. Sumer A, Duran A, Yenilmez G. Isolation of DNA for RAPD analysis from dry leaf material of some Hesperis L. specimens. Plant Mol Biol Rep 2003;21:461a-461f.
12. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-425.
13. Thompson JD, Higgins DG, Gibson TG. Improving the sensitivity of progressive multiple sequence alignment through sequence weighting position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673-4680.
14. Hall TA. BioEdit: a user friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Series 1999;41;95-98.
15. Dixon DP, McEwen AG, Lapthorn AJ, Edward R. Forced evolution of a herbicide detoxifying glutathione trasferase. J Biol Chem 2003;278:23930-23935.
16. Cho HY, Kong KH. Molecular cloning, expression and characterization of a phi-type glutathione S-transferase from Oryza sativa. Pest Biochem Physiol 2005;83:29-36.
17. Mohsenzadeh S, Esmaeili M, Moosavi F, Shahrtash M, Saffari B, Mohabatkar H. Plant glutathione S-transferase classification, structure and evolution. Afr J Biotech 2011;10:8160-8165.
18. Frova C. The plant glutathione transferase gene family: genomic structure, functions, expression and evolution. Physiol Plantarum 2003;119:469-479.
19. Muller LA, Goodman CD, Silady RA, Walbot V. AN9, a petunia glutathione S-transferase required for anthocyanin sequestration is a flavonoid-binding protein. Plant Physiol 2000; 123:1561-1570.
20. Loyall L, Uchida K, Braun S, Furuya M, Frohnmeyer H. Glutathione and a UV light-induced glutathione S-transferase are involved in signaling to chalcone synthase in cell cultures. Plant Cell 2000;12:1939-1950.
21. Basantani M, Srivastava A. Plant glutathione transferases- a decade falls short. Can J Bot 2007;85:443-456.
22. Wagner U, Edwards R, Dixon DP, Mauch F. Probing the diversity of the Arabidopsis glutathione s- transferase gene family. Plant Mol Biol 2002;49:515-532.
23. Cummins I, O’Hagan D, Jablonkai I, Cole DJ, Hehn A, Werck-Reichhart D, Edwards R. Cloning, characterization and regulation of a family of phi class glutathione transferase from wheat. Plant Mol Biol 2003;52:591-603.
24. Cho HY, Lee HJ, Kong KH. A Phi class glutathione S-transferase from Oryza sativa (OsGSTF5): Molecular cloning, expression and biochemical characteristics. J Biochem Mol Biol 2007;40:511-516.
25. Dixon DP, Cummins I, Cole DJ, Edwards R. Glutathione mediated detoxification systems in plants. Curr Opini Plant Biol 1998;1:258-266.
26. Rossini L, Frova C, Pe` ME, Mizzi L, Sari Gorla M. Alachlor regulation of maize glutathione S-transferase genes. Pestic Biochem Physiol 1998;60:205-211.
27. DeRidder BP, Dixon DP, Beussman DJ, Edwards R, Goldsbrough PB. Induction of glutathione S-transferases in Arabidopsis by herbicide safeners. Plant Physiol 2002;130:1497-1505.
28. Scalla R, Roulet A. Cloning and characterization of a glutathione S-transferase induced by an herbicide safener in barley (Hordeum vulgare). Plant Physiol 2002;116:336-344. | ||
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