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TAZIKEH, E., SABOURY, A. A, REZAEI-BEHBEHANI, G., MOOSAVI-MOVAHEDI, A. A., MONAJJEMI1, M.. (1388). A CALORIMETRIC STUDY ON THE INTERACTION OF ZINC ION WITH HUMAN GROWTH HORMONE. , 33(4), 317-327. doi: 10.22099/ijsts.2009.2228
E. TAZIKEH; A. A SABOURY; G. REZAEI-BEHBEHANI; A. A. MOOSAVI-MOVAHEDI; M. MONAJJEMI1. "A CALORIMETRIC STUDY ON THE INTERACTION OF ZINC ION WITH HUMAN GROWTH HORMONE". , 33, 4, 1388, 317-327. doi: 10.22099/ijsts.2009.2228
TAZIKEH, E., SABOURY, A. A, REZAEI-BEHBEHANI, G., MOOSAVI-MOVAHEDI, A. A., MONAJJEMI1, M.. (1388). 'A CALORIMETRIC STUDY ON THE INTERACTION OF ZINC ION WITH HUMAN GROWTH HORMONE', , 33(4), pp. 317-327. doi: 10.22099/ijsts.2009.2228
TAZIKEH, E., SABOURY, A. A, REZAEI-BEHBEHANI, G., MOOSAVI-MOVAHEDI, A. A., MONAJJEMI1, M.. A CALORIMETRIC STUDY ON THE INTERACTION OF ZINC ION WITH HUMAN GROWTH HORMONE. , 1388; 33(4): 317-327. doi: 10.22099/ijsts.2009.2228

A CALORIMETRIC STUDY ON THE INTERACTION OF ZINC ION WITH HUMAN GROWTH HORMONE

Iranian Journal of Science
مقاله 4، دوره 33، شماره 4، اسفند 2009، صفحه 317-327    اصل مقاله (339.55 K)
نوع مقاله: Regular Paper
شناسه دیجیتال (DOI): 10.22099/ijsts.2009.2228
نویسندگان
E. TAZIKEH1؛ A. A SABOURY* 2؛ G. REZAEI-BEHBEHANI3؛ A. A. MOOSAVI-MOVAHEDI2؛ M. MONAJJEMI11
1Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, I. R. of Iran
2Institute of Biochemistry and Biophysics, University of Tehran, Tehran, I. R. of Iran
3Department of Chemistry, Imam Khomeini International University, Qazvin, I. R. of Iran
چکیده
A thermodynamic study on the interaction between zinc ion (Zn2+) and human growth hormone
(hGH) was studied at two temperatures of 27C and 37C in aqueous solution using an isothermal titration
calorimetry. It was found that there is a set of three identical and non-interacting binding sites for Zn2+ ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 1.54 mM and 17.6 kJ mol-1 at 27C and 1.93 mM and 7.1 kJ mol-1 at 37C, respectively. To reproduce the binding parameters of metal ionhormone interaction over the whole range of Zn2+ concentrations a solvation theory was applied. The binding parameters deduced from the solvation model were attributed to the structural change of hGH and its biological activity due to the metal ion interaction
کلیدواژه‌ها
Human growth hormone (hGH)؛ Isothermal titration calorimetry (ITC)؛ Solvation model؛ Zinc ion؛ metal binding؛ Interaction؛ Ligand binding؛ Biothermodynamics؛ Enthalpy of binding؛ Intrinsic equilibrium constant
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